Search PubMed⌕ Search

Biomedical subjects

H Maeda

Publications and source records attributed to H Maeda.

At least 1,135 records · Page 63Linked to original sources
PubMed1985

A novel leukocyte differentiation antigen: two monoclonal antibodies TM2 and TM3 define a 120-kd molecule present on neutrophils, monocytes, platelets, and activated lymphoblasts.

We produced two hybridomas by fusion of mouse myeloma cells with splenocytes from a mouse immunized with the THP-1 human monocytoid leukemia cell line. Two cloned hybridoma cell lines, designated as TM2 and TM3, were obtained. They secreted antibodies against a unique cell surface antigen expressed on all normal peripheral blood monocytes, neutrophilic granulocytes, platelets, and mitogen-induced lymphoblasts, some cells from patients with immature-type lymphoid leukemias. However, the antibodies reacted neither with large numbers of peripheral blood lymphocytes nor with red cells. Cross-blocking studies showed that these monoclonal antibodies recognized the same or a nearly positioned antigen epitope. Immunoprecipitation of THP-1 cell extract with TM2 or TM3 under reducing and nonreducing conditions yielded a specific band of mol wt equal to 120,000 daltons. This determinant appeared to be involved in granulocyte chemotaxis, since neutrophilic granulocytes exposed to TM2 or TM3 showed a significant decrease in chemotaxis toward endotoxin-activated serum. These two monoclonal antibodies did not affect O2- release or luminol-dependent chemiluminescence of neutrophils. Moreover, they did not alter platelet aggregation induced by thrombin. TM2 and TM3 will provide a new reagent in defining the linkage between lymphoid and myeloid differentiation and intermyeloid development.

Acute Disease↗
PubMed1985

[Tumor-targeted chemotherapy with lipid contrast medium and macro molecular anticancer agents theoretical considerations and clinical outcome].

Theoretical considerations for tumor-selective chemotherapy are described which based on the unique character of the tumor neovasculature. Namely, most solid tumors possess four different unique features: hypervasculature, enhanced permeability even to macromolecules, architectural differences, and lack of the lymphatic recovery system. Lipid or lipid contrast medium and macromolecular anticancer agents using prototype drug smancs can be utilized for cancer-selective targeting based on the above four features. Selective targeting with lipid contrast medium with smancs has offered two clinical benefits; definite and pronounced antitumor effect and diagnostic value. These effects can be primarily attributed to the tumor-selective accumulation of the agent, i.e., more than 1,000 times greater in the tumor than in the plasma. As a consequence very few side effects are observed clinically. Primary or secondary hepatoma and lung cancer showed size reduction in more than 90 % of treated patients. Very few side effects such as hematosuppression or inhibited liver function were observed in these cases. Prolongation of life-span was marked in the patients. The above results indicate a new future direction for the development of the tumor-selective chemotherapy.

Animals↗
PubMed1985

[Quantitative analysis of aortic regurgitation using radionuclide phase analysis].

In this study, the clinical utility of phase analysis using gated blood pool scintigraphy to detect aortic regurgitation (AR) was assessed. The study population included 33 patients with AR documented by cineaortography, and 53 patients without AR diagnosed by clinical features and pulsed Doppler echocardiography or cineaortography. Gated blood pool images were stored in modified left anterior oblique views by the multiple gated method (28 frames per beat) after the in vivo labeling of erythrocytes using 25 mCi 99m-Tc. Amplitude was depressed at the subaortic region toward the apex in patients with AR, and occasionally the phase angle occurred earlier in the same region. This is because diastolic filling began earlier and stroke count was decreased more in the subaortic region than in other regions of the left ventricle in examining the regional time-activity curve. Based on this characteristic finding in the amplitude image, the specificity was 81% and the sensitivity was 79% in the diagnosis of AR. The degree of AR was estimated using the amplitude image as follows: grade 1, depression of amplitude localized to the subaortic region; grade 2, depression of amplitude detected from the subaortic region to the midportion of the left ventricle; grade 3, depression of amplitude detected beyond the midportion of the left ventricle. We compared results with those of the radionuclide (RI's classification) and angiographic methods (Sellers' classification) as to the severity of AR. AR could not be detected using the radionuclide method in all four patients with Sellers' grade 1, and in three of nine patients with Sellers' grade 2.(ABSTRACT TRUNCATED AT 250 WORDS)

Aortic Valve Insufficiency↗
PubMed1985

[Distribution of cefotiam dihydrochloride to the bloodless lung].

The concentration of cefotiam (CTM) in the serum and the bloodless lung with time is discussed. Five patients who were undergone pneumonectomy or lobectomy, were given 1 g of CTM intravenously during the operation. Blood samples and lung samples were collected 15, 30, 60, 90, 120, and 300 minutes after the administration. The CTM levels in the blood and the lung samples were measured by the agar well bioassay. The CTM levels in the lung samples were modified as the CTM levels in the bloodless lung by calculating the contained blood volume, as lung samples were proven to have a mean value of 34.2% of blood in weight. The serum CTM level was 73.3 micrograms/ml at 15 minutes after injection and then decreased gradually. The CTM level in bloodless lung was elevated during the first 60 minutes, became equal to that of the blood during next 30 minutes and then exceeded the serum levels thereafter. These results indicate that the distribution of CTM to the bloodless lung was excellent and a satisfactory preventative effect against postsurgical infection could be expected.

Aged↗