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Biomedical subjects

H Müller

Publications and source records attributed to H Müller.

At least 703 records · Page 39Linked to original sources
PubMed1983

[Radiodiagnosis of pathologic processes of the stomach using EDP as a diagnostic aid].

The use of EDP makes it possible to increase the accuracy of the radiodiagnostics of the stomach. The retrospective study is based on 540 X-ray examinations of benign and malignant processes of the stomach which had been diagnosed histologically. It can be proved by means of examination findings that the use of the multivariate discriminatory analysis allows a reliable analysis of the manifold interrelations between the various classes of diagnosis on the one hand and diagnostically usable sign patterns on the other hand so that, in the majority of the classes of diagnosis, the degree to which the diagnostic information issued will increase in comparison to the empirically established radiodiagnoses. The reclassification resulted in the following correct assignments to the various classes: ulcus ventriculi, benign 87 per cent; ulcus ventriculi, malignant - 78 per cent; exulcerated carcinoma - 82 per cent; polyp, benign - 85 per cent; polyp, malignant - 75 per cent; polypoid carcinoma - 91 per cent; stenosis, benign - 85 per cent; stenosis, malignant - 79 per cent; infiltrating carcinoma - 63 per cent. Preconditions for the practical application of EDP in the radiodiagnostics of the stomach are an examination technique and method which exhaust all the available technical possibilities, and an exact interpretation of the radiographic signs.

Diagnosis, Differential↗
PubMed1982

In vivo biosynthesis of the vacuolar proteinases A and B in the yeast Saccharomyces cerevisiae.

Proteinase A and proteinase B, two vacuolar enzymes in Saccharomyces cerevisiae, are synthesized as larger precursors with apparent molecular weights of approximately 52,000 and 42,000, respectively. These precursor molecules are processed to their mature forms of 42,000 molecular weight for proteinase A and 33,000 molecular weight for proteinase B. In the presence of tunicamycin, an inhibitor of the synthesis of protein-asparagine linked carbohydrate moieties, two smaller molecular forms each of precursor and mature proteinase A were synthesized, indicating that proteinase A contains N-linked carbohydrate which is apparently not required for processing. Tunicamycin interferes also with the glycosylation of the proteinase B precursor, whereas no unglycosylated mature proteinase B could be detected.

Aspartic Acid Endopeptidases↗
PubMed1982

Allogeneic marrow transplantation for chronic granulocytic leukemia.

Six patients with Philadelphia-chromosome (Ph' +)-positive chronic granulocytic leukemia were transplanted from their HLA-identical siblings after conditioning with cyclophosphamide and 1'000 rad total body irradiation. All received cyclosporin-A for prophylaxis of Graft-versus-Host disease. All patients showed prompt engraftment and all are cytogenetically and clinically in complete remission. Two patients had transient mild signs of Graft-versus-Host-disease and one patient had unilateral facial nerve paresis of unknown origin. All are ambulatory and well 6-18 months (median 10 months) after transplantation.

Adolescent↗
PubMed1982

Variability of serum indomethacin concentrations after oral and intravenous administration to preterm infants.

Fifteen preterm infants with patent ductus arteriosus and respiratory distress syndrome were given indomethacin (0.2 mg/kg) at 12 h intervals up to three times, either orally or intravenously, in an uncontrolled, non-randomized study. Serum indomethacin concentrations were determined in blood samples taken 12 h after dosing. There was considerable variability in the serum indomethacin concentrations, especially after oral administration, although the mean concentrations after each of the three doses were similar after both oral and intravenous administration. The frequency of closures and transient closures of the ductus arteriosus was also similar for both routes of administration. There was, however, no relation between concentration and effect in individual patients. The sustained exposure to indomethacin which appears to be necessary for ductal closure can sometimes be attained by oral administration.

Administration, Oral↗
PubMed1982

Vascular malformations as a cause of spontaneous spinal epidural haematoma.

Seven cases of spinal epidural haematoma occurred in a series of 2,500 neurosurgical procedures. Five of these seven cases were non-traumatic in origin. In four cases the bleeding could be ascribed to various types of vascular malformations. The clinical and histological features are described, and the relevance of these findings for surgical management is discussed.

Adolescent↗
PubMed1982

Purification and properties of the coupling-factor ATPases F1 from Rhodopseudomonas palustris and Rhodopseudomonas sphaeroides.

The coupling-factor ATPases from photosynthetically grown Rhodopseudomonas palustris and Rhodopseudomonas sphaeroides were purified by the same procedure to homogeneity. Gel chromatography on Sephacryl S-300 Superfine shortened the process of purification and improved its yield. Solubilization of the ATPase from both bacteria was found to be dependent on a specific sonication treatment of the cell suspensions, indicating a very weakly bound F1-ATPase in R. palustris. Depleted chromatophores could be restored in photophosphorylation and membrane-bound ATPase activities by adding the solubilized ATPase protein. The purified enzymes did not show a markedly trypsin-stimulated or dithiothreitol-stimulated activity. Isoelectric focusing and chromatofocusing revealed isoelectric points of 5.0 for both F1-ATPases. The molecular weights were determined by gel chromatography plus high-performance liquid chromatography. Hence, we calculated a molecular weight of 350000 for both F1-ATPases. Sodium dodecylsulfate/polyacrylamide gel electrophoresis revealed five subunits for both enzymes. Kinetic parameters, regarding substrate specificity, the effect of divalent cations, Km and Ki values for the membrane-bound and solubilized ATPases were determined.

Bacterial Proteins↗
PubMed1982

Biosynthesis of virus-specific proteins in cells infected with infectious bursal disease virus and their significance as structural elements for infectious virus and incomplete particles.

It has previously been shown that infectious bursal disease virus is a naked icosahedral particle with a diameter of about 60 nm and a genome consisting of two segments of double-stranded RNA (Müller et al., J. Virol. 31:584-589, 1979). One of the two major structural polypeptides (molecular weight, 40,000) of this virus could not be found in lysates of infected cells; it is derived from a precursor polypeptide demonstrable inside the cells in relatively large quantities and seems to be processed during virus assembly or later. The precursor molecule is regularly present in the infectious virus particle (buoyant density, 1.33 g/ml) in minor proportions, but it represents an outstanding structural element of incomplete noninfectious particles ("top components"; buoyant density, 1.29 g/ml) which contain viral RNA. This type of incomplete particles is mainly produced by chicken embryo fibroblasts in contrast to lymphoid cells from the bursa of Fabricius. Precursor-product relationships also seem to exist in the biosynthesis of the other viral polypeptides. In contrast to some other viruses with a segmented double-stranded RNA genome, none of the structural proteins of infectious bursal disease virus is appreciably glycosylated.

Animals↗
PubMed1982

Recovery of prostaglandin production associated with reopening of the ductus arteriosus after indomethacin treatment in preterm infants with respiratory distress syndrome.

Increased PGE production has been demonstrated in 9 of 17 preterm infants with patent ductus arteriosus (PDA) associated with respiratory distress syndrome (RDS). Inhibition of PGE production in eight preterm infants with PDA and RDS was associated with marked improvement in the respiratory and circulatory function of all of them. However, in six of them this effect was only transient. In the posttreatment period of five and a half days reopening of the ductus arteriosus was frequently associated with increased PGE production and a drop of indomethacin serum levels. Three of these six infants were transferred for surgical ligation whereas the other three were successfully treated with a second course of indomethacin. However, the margin between closure of the ductus arteriosus and the deterioration of kidney function in preterm infants treated with a presently recommended indomethacin dosage was narrow. In conclusion, until an acceptable therapeutic serum level of indomethacin for ductal closure in preterm infants has been established and the duration of effective prostaglandin synthesis inhibition is known, it is too early for a general recommendation of a dosage regime of indomethacin for the pharmacological closure of PDA in infants with RDS.

Child↗
PubMed1982

[Influence of different preinjections on unfavourable side-effects of succinylcholine-induced block (author's transl)].

Pretreatment with a subparalytic dose of a nondepolarizing relaxant is used to prevent side-effects of the succinylcholine (Sch) dose needed for intubation. On the other hand small doses of Sch (self-taming) or lignocaine are recommended to avoid in part reversal of the Sch-induced block caused by nondepolarizing relaxant. 50 patients received different preinjections before Sch-induced relaxation: 2 mg alcuronium, n = 10; 1 mg pancuronium, n = 10; 0.1 mg/kg bw Sch, n = 10; 1 mg/kg bw lignocaine, n = 10; no pretreatment, n = 10. Injection of small doses of Sch or lignocaine prior to the Sch dose needed for intubation, offered no advantage in comparison to the conventional method of pretreatment with nondepolarizing relaxant, concerning intensity and duration of the relaxation, conditions for intubation and muscle fasciculations. After preinjection of Sch or lignocaine a similar rise of serum potassium was observed as without preinjection (0.4-0.5 mEq/l).

Humans↗
PubMed1982

[Sarcomatous degeneration in Recklinghausen's neurofibromatosis].

During a 6-year period only a single case of sarcomatous transformation in neurofibromatosis (v. Recklinghausen's disease) was found among 1561 autopsies. The clinical course and histopathological changes of this rare case are described. The incidence of sarcomatous transformation seems to be less than 3%, in contrast to some data reported in the literature. These fibrosarcomas of the nerve sheaths usually originate in subfascial nodes and in other clinically poorly accessible locations. Some cases in the literature indicate genetic factors to be responsible for the sarcomatous transformation. Therefore, patients with such a family history should be followed closely.

Humans↗