Biomedical subjects
Publications and source records attributed to H M Brandt.
Use of the fluorescence-activated cell sorter proved to be an accurate and highly efficient means for cloning Babesia parasites. These qualities were examined by separating a mixed population of Babesia-infected bovine erythrocytes composed of two isolates with different karyotypes. Direct evidence of polymorphism was detected during comparison of the resultant clones.
Random amplified polymorphic DNA analysis provides characteristic fingerprints for Babesia bovis and B. bigemina. This genetic profile reflects similarities and minor differences between closely related regional isolates and the greater diversity representative of species from distant geographic locations.
A commerically available inexpensive calculator was modified and mounted next to one of the display oscilloscopes on a scintillation-camera console. This enabled the technologist to dial in each patient's identification number, which then appeared on every frame of the 35-mm film used. By using this device, labeling errors have been reduced to a minimum.
The properties of a new anterograde tracer, biotin-dextran (BD), are reported. Iontophoretic or pressure injections of BD in the spinal cord of rats and monkeys revealed terminal-like BD label in many regions of the brain. The BD label was visualized by an avidin-biotin reaction combined with diaminobenzidine, with and without nickel enhancement. This reaction resulted in permanent label and revealed the fine morphology of terminal boutons and en-passant endings. Biotin-dextran is an excellent anterograde tracer that can also be visualized at the electron microscopic level, used in combination with other retrograde and anterograde tracers and with immunohistochemical labeling of neurotransmitters.