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Biomedical subjects

H Luppa

Publications and source records attributed to H Luppa.

At least 55 records · Page 3Linked to original sources
PubMed1976

The histochemical demonstration of choline acetyltransferase by semipermeable membrane technique.

The application of the semipermeable membrane technique in light microscopical demonstration of choline acetyltransferase is described. The method founds upon earlier developed lead salt techniques. Use of semipermeable membranes fully prevents any loss of enzyme by dissolvement or inactivation during fixation. Addition of NaCl to the incubation medium markedly increases the activity of choline acetyltransferase.

Acetyltransferases↗
PubMed1976

[Localization and behaviour of hydrolases and transmitter enzymes in the dorsal Corpus geniculatum laterale of the rat after unilateral enucleation (author's transl)].

In the Corpus geniculatum laterale [pars dorsalis] (Cgl d) of the rat the distribution of the following enzymes is described on the light microscopical level: acid phosphatase, nonspecific esterases, Thiaminepyrophosphatase (TPPase), cholinesterases (AchE and ChE), GABA-alpha-ketoglutarat-transaminase (GABA-T), monoaminooxidase (MAO) and leucin-aminopeptidase. The acid phosphatase and TPPase are localized in the pericarya of different cell types of the Cgl d. The nonspecific esterases are present intracellularly and in the neuropil. With the GABA-T-reaction single cells of Cgl d are stained. The leucine aminopeptidase could not be demonstrated in the Cgl. The nonspecific cholinesterase is localized in the vessel walls. AChE and MAO are present in different fiber structures in the Cgl. Electron microscopical investigations of AChE showed a localization of the enzym in the extracellular room, intraaxonal and in the synaptic cleft. Separation of ChE in polyacrylamid gel resulted in 2 bands for the AChE and 1 band for the nonspecific ChE. After left side enucleation the reaction of the enzymes acid phosphatase, nonspecific esterases cholinesterases and TPPase was proofed topochemically at the section and biochemically at the homogenate. A decrease of enzyme activity in the enzymes acid phosphatase, nonspecific esterases and AchE is shown. The alterations are discussed in connection with the transneuronal degeneration.

4-Aminobutyrate Transaminase↗
PubMed1976

[Morpho-histochemical investigations about the connection of the mesencephalic reticular formation (Area cuneiformis) to the subcortical structures of the visual system (author's transl)].

There was investigated the connection of the mesencephalic reticular formation (Area cuneiformis) with subcortical structures of the visual system. After coagulation of the reticular formation we studied the connections by degeneration and enzymhistochemistric methods. Terminal degeneration in different intensity was observed in the ventral part of the lateral geniculate in the nucleus lateralis posterior, the pretectal region and in the lower laminae (IV ... VII) of the superior colliculus. The findings are mainly ipsilateral. A direct projection to the dorsal part of the lateral geniculate could not be observed. By enzymhistochemic methods we localized the AchE intracellular in the rostral part of the area cuneiformis. After destruction of this area there was observed a clear diminishing of the AchE activity in the ventral lateral geniculate and in the area pretectalis. These results were confirmed by biochemical methods.

Acetylcholinesterase↗