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Biomedical subjects

H Liu

Publications and source records attributed to H Liu.

At least 1,171 records · Page 65Linked to original sources
PubMed1992

Ceftibuten and trimethoprim-sulfamethoxazole for treatment of Shigella and enteroinvasive Escherichia coli disease.

In a prospective randomized study at two clinical sites, ceftibuten was compared with trimethoprim-sulfamethoxazole (TMP-SMX), both given orally for a period of 5 days, for the treatment of dysentery. Twenty-two children were found to have bacillary dysentery caused by Shigella and/or enteroinvasive Escherichia coli. All organisms isolated were susceptible to ceftibuten; 6 of 20 Shigella strains and 4 of 5 enteroinvasive E. coli were resistant to TMP-SMX. The diarrhea persisted for a mean (+/- SD) period of 2.4 +/- 1.4 days in the ceftibuten-treated patients vs. 3.4 +/- 1.7 days in the TMP-SMX-treated patients. The duration of fever was similar for both treatment groups. Patients treated with ceftibuten or TMP-SMX had equivalent clinical responses unless the pathogen was found to be TMP-SMX-resistant. Those who were randomized to receive TMP-SMX but who were eventually found to have TMP-SMX-resistant organisms had significantly more stools at days 3, 4 and 5 (P less than 0.02 to less than 0.00006) with more watery consistency for these days (P less than 0.02 to less than 0.005) compared to patients treated with ceftibuten. No clinical relapses were reported and no drug-related side effects were observed. We conclude that ceftibuten is at least as effective as TMP-SMX in the treatment of diarrhea caused by Shigella and enteroinvasive E. coli in children.

Adolescent↗
PubMed1992

Effect of particle lipid content on the structure of insect lipophorins.

Four distinct subspecies of the major insect lipoprotein, lipophorin, that range in overall lipid content from 20 to 51% of the particle mass, were isolated from the hemolymph or oocytes of the tobacco hornworm, Manduca sexta. Examination of these subspecies by electron microscopy revealed distinctive morphologies. Adult high density lipophorin (HDLp-A) was found to be an approximately spherical particle with a diameter of 15 +/- 1 nm, while HDLp-Wanderer 1 (W1), was more rectangular in shape and had a distinct cleft extending into the particle at one end. In the case of HDLp-Wanderer 2 (W2) the cleft was deeper and wider than that in HDLp-W1. In egg very high density lipophorin (VHDLp-E) the cleft was increased in size to the extent that the particle had an overall crescent-like conformation. Circular dichroism spectroscopy of the three lipophorin subspecies that contain only apolipophorin I and II revealed that only minor differences in the global protein secondary structure occur as the particle lipid content is decreased. The VHDLp-E apolipoproteins are an exception in that, while having the same alpha-helix content as HDLp-W1 and HDLp-W2, they contain less beta-structure and correspondingly more random coil. Limited digestion of the apolipoprotein components of the lipophorin subspecies with trypsin revealed that as the lipid content of the particles decreases the susceptibility of the apolipoprotein to proteolytic degradation increases. Likewise, tryptophan fluorescence quenching experiments demonstrated that the relative exposure of lipophorin apolipoprotein tryptophan residues also increases as the particle lipid content decreases.(ABSTRACT TRUNCATED AT 250 WORDS)

Animals↗
PubMed1991

Conventional antigen and superantigen may be coupled to distinct and cooperative T-cell activation pathways.

CD4+ T cells are equipped to detect two major classes of ligands. Infectious microbial agents, including bacteria and retroviruses, carry a class of proteins termed superantigens that are recognized by the T-cell receptor in association with class II products of the major histocompatibility complex. Proteins expressed by other cells and organisms are processed by macrophages into peptides that are presented to CD4+ T cells by class II molecules. We have examined CD4+ T-cell clones that proliferate vigorously in response both to conventional peptide antigens and to bacterial or retroviral superantigens. The response to peptide antigen is characterized by a rapid and sustained increase in the levels of intracellular free Ca2+ and a vigorous cytokine response. In contrast, the proliferative response of these clones to bacterial or retroviral superantigen is not accompanied by detectable increases in intracellular Ca2+ or by significant cytokine production. Further analysis of T-cell activation indicates that interaction of a single T-cell receptor with the two types of ligand may be coupled to functionally distinct signaling pathways that interact in a synergistic fashion to achieve T-cell activation.

Animals↗
PubMed1991

Displacement of apolipophorin III from the surface of low density lipophorin by human apolipoprotein A-I.

A hybrid low density lipophorin particle (LDLp) was prepared by incubation with human apolipoprotein (apo) A-I in vitro. ApoA-I associated with LDLp in a concentration dependent, saturable manner which was accompanied by dissociation of apolipophorin III (apoLp-III). The apoA-I hybrid LDLp had the same lipid composition, density and morphology as native LDLp indicating that displacement of apoLp-III by apoA-I did not affect its structural properties. The molar ratio of apoLp-I:apoLp-II:apoLp-III was maximally reduced from 1:1:16 to 1:1:2 in native versus hybrid LDLp with the latter particle binding 7 molecules of apoA-I. The inability of apoA-I to displace the remaining 2 apoLp-III supports the concept that these apoLp-III molecules are not equivalent to the other fourteen. Native and hybrid LDLp particles were both metabolized to high density lipophorin in vivo. The displacement reaction represents a novel method for the production of apolipoprotein hybrids of LDLp and the results indicate that apoA-I has an inherently higher affinity for lipid surfaces than apoLp-III.

Animals↗
PubMed1991

Role of lipid transfer particle in transformation of lipophorin in insect oocytes.

Oocyte development in Manduca sexta involves the deposition of large amounts of lipoprotein-derived lipid. One source of this lipid is hemolymph high-density lipophorin-adult (HDLp-A) which, upon entry into the oocyte, is transformed into a lipid and apolipophorin III deficient product particle, egg very-high- density lipophorin (VHDLp-E; density = 1.24 g/ml; Kawooya et al. (1988) J. Biol. Chem. 263, 8740-8747). An in vitro model of this transformation has been established using human low-density lipoprotein (LDL) as acceptor of HDLp-A associated lipid in a reaction catalyzed by isolated M. sexta hemolymph lipid transfer particle (LTP). Facilitated vectorial net transfer of lipid from HDLp-A to LDL resulted in formation of a very-high-density lipophorin (VHDLp) product with a density and apolipoprotein content similar to that of VHDLp-E. Lipid was found to comprise 25% of the VHDLp particle mass, whereas over 50% of HDLp-A mass is lipid. Based on these observations it was hypothesized that a lipid transfer factor may be present in M. sexta oocytes and function in the transformation of HDLp-A to VHDLp-E in vivo. Transfer activity was present in the buffer soluble fraction of oocyte homogenates and purification of the active material revealed a catalyst with electrophoretic and immunological properties identical to hemolymph LTP. Incubation of 125I-HDLp-A with an M. sexta oocyte homogenate resulted in transformation of the radiolabeled lipoprotein to a density corresponding to that of VHDLp-E. When the incubation media was preincubated with anti-LTP IgG this conversion was inhibited to a large extent. Inhibition was relieved, however, by addition of exogenous LTP. The results provide the first demonstration of M. sexta LTP in a tissue other than hemolymph and support the concept that LTP-catalyzed lipid transfer plays an integral role in the conversion of HDLp-A to VHDLp-E in vivo.

Animals↗
PubMed1991

Microsurgical fertilization and teratozoospermia.

Semen parameters were correlated with the outcome of partial zona dissection (PZD) in 42 couples with male factor infertility. Although fertilization rates were reduced, 12% of the embryos implanted following replacement. Spermatozoa from teratozoospermic sperm populations were able to fuse with oocytes following zona penetration through the artificial gaps. PZD followed by insemination with less than 5% normal spermatozoa led to 20 embryos which, upon replacement, did not implant. Motility and sperm count were not clearly correlated with the outcome of PZD and are therefore less useful indicators for patient selection. Teratozoospermic patients who previously failed to fertilize were compared to a group of similar patients who had not attempted IVF before. Although fertilization was significantly improved in first-time patients, 41% of the patients whose spermatozoa were initially unable to fertilize had at least one embryo when PZD was performed. Several pregnancies were established in this group. Subzonal sperm insertion (SZI) and PZD were compared in 19 patients using sibling oocytes. A significant fraction of spermatozoa from infertile men were able to fuse with the oolemma when directly inserted into the perivitelline area. Using a sucrose solution to shrink the ooplasm, only 1% of the oocytes were damaged during SZI. Monospermic fertilization rates following PZD and SZI were 15 and 16%, respectively. Both micromanipulation methods were successful in most patients. However, in two small groups of patients, only one technique resulted in fertilization.

Female↗
PubMed1991

Role of L3T4+ and 38+ T-cell subsets in resistance against infection with Treponema pallidum subsp. pertenue in hamsters.

The protective immunity conferred by T-cell subsets against infection with Treponema pallidum subsp. pertenue was studied. We demonstrated that hamster T cells can be separated into two subsets by monoclonal antibody (MAb) GK 1.5 (anti-L3T4) and MAb 38. Eighty-five percent of hamster thymocytes were L3T4+ and 87% were 38+ cells; 84% were dual positive for MAbs anti-L3T4 and 38. In the peripheral lymph nodes, however, the L3T4+ and 38+ T cells were mutually exclusive according to two-color immunofluorescence analysis. The two T-cell subsets were found to be functionally distinct according to their secretion of interleukin 2 (IL-2) when stimulated with concanavalin A. The L3T4+ cells secreted IL-2 and had characteristics of T helper cells, while the 38+ cells did not secrete IL-2 and appeared to be T cytotoxic-suppressor cells. Transfer of 4 x 10(6) helper or cytotoxic-suppressor T lymphocytes from T. pallidum subsp. pertenue-immune hamsters protected irradiated naive hamsters against challenge with this subspecies. IL-2 production could still be detected in the irradiated recipients 12 days after irradiation of naive recipients, although at a low level. This suggests that the remaining lymph node cells could support the survival and expansion of the infused cytotoxic-suppressor T cells. No accumulation of macrophages was observed in regional lymph nodes of immune T-cell recipients within 10 days of infection. Instead, there was an influx of polymorphonuclear neutrophils in all animals injected with T. pallidum subsp. pertenue. This report demonstrates that hamster T cells can be separated into two phenotypically and functionally distinct subsets and that both T-cell subsets confer protection against challenge with T. pallidum subsp. pertenue.

Animals↗
PubMed1991

Increased nasal resistance induced by the pressure-flow technique and its effect on pressure and airflow during speech.

Although the validity of the pressure-flow technique has been verified in a number of laboratories, some questions still remain. The purpose of this study was to determine whether the procedures involved in estimating orifice size affect the pressure and airflow variables being measured. Twenty subjects with demonstrated velopharyngeal inadequacy on pressure-flow testing (VPO greater than or equal to 0.10 cm2) were assessed under two contrasting conditions. Subjects were asked to produce (p) in the word "hamper" with a) one nostril occluded by a cork as in pressure-flow testing and b) both nostrils patent. The results indicate that the increased nasal resistance resulting from occlusion of one nostril does not appreciably affect pressure and airflow associated with plosive consonant production in patients with velopharyngeal inadequacy.

Adolescent↗
PubMed1991

Treatment of small cell lung cancer.

During a 28-year period, 110 patients with small cell lung cancer were treated at our hospital. Fifty-nine patients received surgery, and 58 patients (including 7 cases after exploratory thoracotomy) received chemotherapy and/or radiotherapy. The 5-year survival rate of the surgically treated group was 17%, while none in the non-surgical group survived for more than 5 years.

Adult↗
PubMed1991

[Comparison of Spatholobus suberectus Dum, Euonymus alatus (Thunb.) Sieb. and Eupolyphaga sinensis Walker on regulation of plasma lipid].

The Spatholobus suberectus (SS) of hexue type, the Euonymus alatus (EA) of huoxue type and the Eupolyphaga sinensis (ES) of poxue type were selected and their influence on plasma lipid in the experimental hyperlipidemia quails was observed. The ES could raise plasma HDL-C/TC ratio and increase LCAT activity. The SS could raise plasma HDL2-C/HDL3-C ratio. The effect of EA on plasma HDL-C/TC, HDL2-C/HDL3-C and LCAT levels was between SS and ES. All the three huoxue huayu Chinese drugs could lower plasma HDL3-C level and slow down the progress of atherosclerosis to a certain degree. The above-mentioned results show that certain orders exist between the action range of huoxue huayu drugs and their effect on regulating plasma lipid.

Animals↗
PubMed1991

Prevention of tumour production in rats fed aminopyrine plus nitrite by sea buckthorn juice.

Three groups of Wistar rats were fed a diet containing aminopyrine (0.2%) and NaNO2 (0.2%) and either tap-water (controls), sea buckthorn juice or ascorbic acid solution ad libitum for 38 weeks. All 17 rats given tap-water developed tumours in the liver, and six developed tumours in the lungs and four in the kidneys; in rats given sea buckthorn juice, 15 had tumours of the liver, 11 of the lungs and two of the kidneys; the incidences in rats given ascorbic acid were 18/18, 6/18 and 4/18, respectively. The average life span of the group given sea buckthorn juice was 270 days, which was significantly longer (p less than 0.01) than those of rats given tap-water (195 days) or ascorbic acid (220 days). Microscopic examination of the livers of rats receiving sea buckthorn juice showed fewer foci of carcinogenesis than those of the control and the ascorbic acid groups. The results suggest that sea buckthorn juice can block the endogenous formation of N-nitroso compounds more effectively than ascorbic acid and thereby prevent tumour production.

Aminopyrine↗