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Biomedical subjects

H Link

Publications and source records attributed to H Link.

At least 523 records · Page 29Linked to original sources
PubMed1978

Comparison between agarose gel electrophoresis and isoelectric focusing of CSF for demonstration of oligoclonal immunoglobulin bands in neurological disorders.

Isoelectric focusing and agarose gel electrophoresis of CSF and serum revealed similar frequencies of oligoclonal bands in multiple sclerosis (100% with both methods), infectious CNS disorders (38 and 23%) and other neurological diseases (8 and 10%). In selected cases with unsure CSF oligoclonal bands on agarose gel electrophoresis, isoelectric focusing displayed definite oligoclonal bands. In contrast to agarose gel electrophoresis, isoelectric focusing revealed evidence for oligoclonal bands in serum as well as in CSF in 41% of the multiple sclerosis patients, indicating diffusion from CSF to serum. In 4 cases with gammaglobulin bands appearing in both CSF and serum on agarose gel electrophoresis, isoelectric focusing revealed normal CSF and serum protein patterns at pH above 6.4 where most IgG is migrating.

Albumins↗
PubMed1978

Influence of measles virus antigen on leukocyte migration in multiple sclerosis and controls.

The leukocyte migration inhibiton test was used in multiple sclerosis patients and in healthy controls for investigation of sensitization of blood lymphocytes against preparations of measles virus, mumps, NDV virus, and PPD. Regardless of antigen used, no significant differences were found between the groups. There was a strong correlation between the leukocyte migration inhibition obtained with measles virus antigen and with PPD. The leukocytes from MS patients as well as from healthy controls showed a significantly more pronounced inhibiton when a Tween 80 and ether-treated preparation of measles virus was used, compared with the whole measles virus antigen. No correlations were found between the degree of leukocyte migration inhibition and the antibody titers against different components of measles virus in individual samples.

Adult↗
PubMed1978

Mitogen stimulation of cerebrospinal fluid lymphocytes in aseptic meningitis.

The cell responses to different polyclonal lymphocyte activating substances were studied in 15 patients with aseptic meningitis by analysis of lymphocyte proliferation. The in vitro response to phytohaemagglutinin (PHA), concanavalin A (Con A) and poke weed mitogen (PWM) was investigated in blood and CSF lymphocytes simultaneously. The blood lymphocytes responded stronger than the corresponding number of CSF lymphocytes. These cells were, however, always capable of a response, suggesting that they were immunologically competent. No predominance of either PHA or Con A populations of T lymphocytes could be demonstrated in CSF compared to blood by this technique. When culture conditions were changed from the optimal cell or mitogen concentrations, the decrease in proliferation of the CSF lymphocytes was more pronounced than that of blood lymphocytes.

Adult↗
PubMed1978

Cerebrospinal fluid and serum immunoglobulins and antibody titers in mumps meningitis and aseptic meningitis of other etiology.

Cerebrospinal fluid (CSF) and serum from 19 patients with mumps meningitis and 19 patients with meningitis of other etiology were investigated on two or more occasions for at least 1 month after onset. Intrathecal synthesis of immunoglobulin (Ig) G was found in 55%, of IgA in 26%, and of IgM in 24% of the patients. Oligoclonal Ig was demonstrable by agarose gel electrophoresis in 37% of the patients, mostly already during the first week after onset, and could persist for years. Mumps virus antibody synthesis within the central nervous system occurred in 37% of the mumps meningitis patients. The inflammatory reaction within the central nervous system as reflected by mononuclear pleocytosis, Ig synthesis, and oligoclonal Ig was not correlated to the clinical course. The blood-brain barrier was evaluated by determination of the CSF total protein, CSF/serum albumin ratio, and CSF/serum alpha2-macroglobulin ratio. A significant correlation was found among these three parameters. Persistence of the elevated CSF/serum albumin ratio seems to influence prognosis, and this parameter is recommended for evaluation of the blood-brain barrier function.

Antibodies, Viral↗
PubMed1978

[Angiographic features of trophoblastic tumors (author's transl)].

Trophoblastic tumors are characterized by typical angiographic features with marked pathological vascularization, arteriovenous shunts and early filling of veines. If the HCG-titer increases after removing of a hydatidiform mole pelvic angiography is indicated in cases of negative histological finding also. In 3 cases the typical angiographic feature was found. Two times remaining changes of gravidity are histologicaly found on an abrasio. In 1 case with increased HCG-titer and negative histological finding a trophoblastic tumor was ascertained angiographicaly. Diagnostic value of pelvic angiography is demonstrated by above mentioned cases.

Abortion, Therapeutic↗
PubMed1978

[Indication for surgical therapy of tubo-ovarian abscess].

It is reported about the indication to operative therapy, therapy-shape and the going of cure of 30 patients suffering from tuboovarian abscesses. At the beginning there is the conservative therapy with general measures immediately dose of antibiotica without waiting for the germ and resistence determinations. However the well timed operative therapy under conditions as well as possible is deciding. As a rule the abdominal exstirpation of the uterus with both adnexe is practiced in order to come to a complete removal of the infection hearth. --The postoperative cours war only febril in one third of the treated women. In 86,7% the laparotomy scare healed per primam intentionem. We didn't observe an extension of the infection post operationem and there were not any complications with letal exit.

Abscess↗
PubMed1978

[Significance of after care in asymptomatic pregnancy bacteriuria and pyelonephritis gravidarum].

In the period from 1974 to 1976 3465 deliveries were performed in the "Landesfrauenklinik". During pregnancies there were started 10,7% urological and nephrological complications with the asymptomatic bacteriuria were predominating at a rate of 6,1%. --Considering the high rate of chronic complications in all types of urinary tract infections, long-term dispensary care should be postulated.

Anti-Bacterial Agents↗
PubMed1977

Principles of albumin and IgG analyses in neurological disorders. III. Evaluation of IgG synthesis within the central nervous system in multiple sclerosis.

Fifty-nine multiple sclerosis patients were investigated with regard to blood-brain barrier function by determining the CSF-protein and the CSF/S albumin ratio. Abnormal values were found in 19% and 32% respectively. The occurrence of CSF-IgG elevation due to synthesis within the CNS was analysed by determining the CSF IgG/protein and CSF IgG/albumin ratios and the IgG-index = (CSF/S IgG ratio)/(CSF/S albumin ratio). Increased values were found in 75%, 80% and 86%, respectively. Oligoclonal IgG was found in CSF by agarose gel electrophoresis in 88% of the patients. The IgG-index is the parameter recommended for the demonstration of an IgG elevation in CSF due to synthesis within the CNS.

Adult↗
PubMed1976

Immunoglobulin abnormalities and measles antibody response in chronic myelopathy.

Patients with chronic myelopathy of unknown origin were separated into two groups on the basis of presence or absence of oligoclonal immunoglobulin G (lgG) in cerebrospinal fluid (CSF). Thirty-nine of 48 patients (81%) with oligoclonal lgG in CSF had measles virus antibodies in the CSF and 31 (65%) showed reduced serum/CSF measles virus antibody ratios, in comparison with the corresponding ratios of adenovirus, group-specific, penton hemagglutination-enhancement and poliovirus neutralization-enhancement antibodies. Of 25 patients with myelopathy, but without oligoclonal lgG in their CSF, three had detectable titers of measles CSF antibodies and one of these had a greatly reduced serum/CSF ratio. The conditions of patients with chronic myelopathy of unknown origin and oligoclonal lgG in CSF may be diagnosed as probable multiple sclerosis (MS), in contrast to patients with this disease who lack oligoclonal lgG IN CSF. However, the clinical features of the disease in these two groups do not differ substantially.

Adult↗
PubMed1976

HLA haplotypes in families with high frequency of multiple sclerosis.

Eight families from southern Sweden having two or more members with multiple sclerosis (MS) were typed for various alleles of the HLA system. The MS patients within each family shared one major histocompatibility system (MHS) haplotype, which was identical to the hitherto-described MS-associated haplotype A3B7Dw2 only in two of the families. Healthy relatives of MS patients were often found to carry the same haplotype as the affected members, which makes an estimate of the degree of penetrance of disease in individuals carrying the MS-predisposing MHS-linked gene possible.

Adult↗
PubMed1976

Immunoglobulin abnormalities in multiple sclerosis. Relation to clinical parameters: disability, duration and age of onset.

Cerebrospinal fluid (CSF) and serum from 35 pairs of multiple sclerosis (MS) patients were analysed as regards mononuclear pleocytosis, concentrations of total protein, immunoglobulin G and A and beta-trace protein, and kappa:lambda ratios, as well as the serum/CSF ratios of IgG and albumin. The disability of the patients differed, whereas the age and the duration of the disease were similar in each pair. Similar analyses were also performed on CSF and serum from 72 patients, who were subdivided according to age at onset and severity of the disease. The highest mean values of the CSF-IgG and the lowest mean values of the serum/CSF IgG ratios were found in the more disabled patients. CSF immunoglobulin abnormalities were encountered more often and were more pronounced in the patients with the most malignant course of the disease, i.e., in those with severe disability after a short duration of the disease (less than 10 yr) and in severely disabled patients with an early age at onset of the disease(less than 25 yr). Contrarily, normal mean values of CSF-IgG concentrations and serum/CFS/IgG ratios were found in the groups of patients without disability after a duration of the disease of 10 years or more, and patients without disability and an early age at onset of the disease (less than 25 yr). The observations indicate that the immune response is most vigorous in disabled patients with a short duration or with an early age at onset of the disease. MS patients with a late age at onset (greater than 35 yr) showed a less pronouced immune response within the CNS, irrespective of the occurrence of disability. The most disabled patients also showed the most severe blood-brain barrier damage as manifested by high mean values of total protein in CSF and low serum/CSF albumin ratios. The patients with severe disability and a long duration of the disease (greater than 10 yr) had the highest content of beta-trace protein in the CSF, probably as a sign of destruction of brain matter.

Adult↗
PubMed1976

Antibodies to Epstein-Barr virus and cytomegalovirus in Guillain-Barre syndrome.

Twenty-three patients with Guillain-Barré syndrome (GBS) were investigated for serum antibody titres against Epstein-Barr virus capsid antigen (EBV-VCA), cytomegalovirus (CMV) and herpes simplex virus type 1 (HSV1). The geometric mean EBV-VCA titre was 45 in GBS, compared with 15-22 in healthy adults and 60 in sarcoidosis. Nine of the 15 GBS patients (60%) from whom multiple serum specimens were available, displayed a four-fold variation in antibody titre to EBV-VCA during the course of their disease. The geometric mean titres against CMV and HSV were also slightly higher in GBS compared with controls, but no titre variations were observed. Serial samples of both serum and cerebrospinal fluid (CSF) were available from 2 patients. Serum antibody titres against EBV were significantly higher in both during the initial phase of the disease. Serum antibody titres against CMV were also significantly elevated in 1 patient, but not until the second month after onset. The CSF antibody titres against EBV were higher in the beginning of the disease compared with the values during convalescence, but the titres observed were low. No measurable CSF antibody titres against CMV were found. The observation of varying serum antibody titres against EBV during the course of GBS can be interpreted as primary infections or as manifestations of reactivated latent virus infections. Further studies of the pathogenetic significance of this for the aetiology of the disease are warranted.

Adult↗
PubMed1976

Relation between Genetic markers and oligoclonal IgG in CSF in optic neuritis.

Thirty patients with acute, unilateral optic neuritis (ON), where re-examination after a mean observation period of 5 years did not reveal any aetiology, were investigated with regard to laboratory abnormalities frequently observed in multiple sclerosis. Eleven patients had oligoclonal IgG in CSF. In 5 of these a measles virus antibody response within the CNS was demonstrable. The remaining 19 patients did not display oligoclonal CSF IgG, nor an antibody response. The major histocompatibility antigens HL-A3 and HL-A7 occurred at similar frequencies in ON and in controls, irrespective of the presence of oligoclonal CSF IgG. The HL-A7 associated MLC determinant LD-7a occurred in ON at a frequency between that observed in controls and in MS. However, an association of the same magnitude as observed in MS was found between ON with oligoclonal CSF IgG and the presence of LD-7a. This association was absent in those ON patients who lacked oligoclonal CSF IgG. The present data indicate that the finding of oligoclonal CSF IgG may increase the risk of developing MS.

Adolescent↗