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Biomedical subjects

H Lin

Publications and source records attributed to H Lin.

At least 469 records · Page 26Linked to original sources
PubMed1985

Comparison of several control standard endotoxins to the National Reference Standard Endotoxin--an HIMA collaborative study.

A collaborative study, initiated under the auspices of the Health Industry Manufacturers Association (HIMA), was designed to establish the relationship of Escherichia coli O55:B5 endotoxin (the control standard endotoxin of HIMA and the Food and Drug Administration's Office of Medical Devices) to the U.S. National Reference Standard Endotoxin and to two internationally used control standard endotoxins. By using two Limulus amoebocyte lysate test systems, it was established that the E. coli O55:B5 endotoxin lot originally used by HIMA and the Office of Medical Devices to establish Limulus amoebocyte lysate release test criteria for pyrogen testing of medical devices contains approximately 4.5 endotoxin units (EU) per ng. Thus, the 1.0-ng/kg endotoxin dose limit currently established for medical devices is approximately the same as the 5.0-EU/kg endotoxin limit (on an activity basis) established by several other Food and Drug Administration agencies for human and animal parenteral drugs and biological products.

Endotoxins↗
PubMed1984

Treatment of resistant malignant lymphoma with cyclophosphamide, total body irradiation, and transplantation of cryopreserved autologous marrow.

Twenty-seven patients with malignant lymphoma in whom primary chemotherapy had failed and the prognosis was poor were treated with cyclophosphamide, total body irradiation, and transplantation of cryopreserved autologous marrow. The median time to recovery of more than 500 neutrophils per microliter and more than 10,000 platelets per microliter was 18 and 24 days, respectively. Complete remission was achieved in 15 patients (56 per cent), five of whom were in continuous remission at this writing 19 to 71 months after transplantation without further therapy and one of whom was alive in a subsequent remission at 20 months. Fifteen patients died of lymphoma, three of interstitial pneumonitis, two of sepsis, and one of congestive heart failure. This experience shows that intensive therapy and autologous-marrow transplantation can produce prolonged remissions in patients with malignant lymphoma in whom conventional chemotherapy has failed.

Adolescent↗
PubMed1982

The skeleton, an important site of lipogenesis in the chick.

Studies in vitro and in vivo with chicks using 14C-1-acetate have shown that the bone could be an important site of lipogenesis. The in vitro relative activities of various bones in incorporating 14C into fatty acids varied from one tenth to one third of hepatic activity. The bone marrow activity attained about two thirds that of the liver. In vitro and in vivo activities of bones were highly correlated (r = 0.902). The activity of compact parietal bones was the lowest. Estimates of lipogenesis in vivo in various tissues (% of total incorporation) were as follows: liver, 45; skeleton, 23; skin and small intestine, 6. Incorporation by muscles, adipose tissue and kidneys were negligible.

Acetates↗
PubMed1982

In vitro effect of LH-releasing factor on the content of cyclic cytidine 3' 5' monophosphate (c-CMP) in the rat anterior pituitary.

The effect of LH-RF on pituitary content of cyclic cytidine 3' 5' monophosphate (c-CMP), one of the newly detected cyclic nucleotides, was examined by the RIA procedure. In results showed that the pituitary content of c-CMP was lower than in various other organs of the rat. When incubated in vitro in the presence of 0.5 ng LH-RF/ml incubation medium, c-CMP content of the anterior pituitaries was reduced slightly, but the difference was not statistically significant when compared with the controls. No difference in c-CMP content was observed between the controls and the 5 ng LH-RF/ml group. C-CMP content in the rat anterior pituitary tissue did not change significantly during an in vitro time-course study (5, 15, and 30 min.) in the presence of 5 ng LH-RF/ml. In contrast, c-AMP content of the pituitary was significantly (P less than 0.05) elevated by the stimulation of 5 ng of LH-RF/ml at a 15 minutes of incubation. These data suggest that c-CMP content, unlike c-AMP, might not be changed significantly by hypothalamic gonadotropin releasing hormone.

Animals↗
PubMed1981

Inhibitor of peritoneal mononuclear phagocyte colony forming cells.

We investigated an inhibitory factor present in mouse L-cell conditioned medium. This soluble factor, colony inhibitory factor (CIF), selectively inhibits the clonal growth of mouse peritoneal exudate (PE) macrophages in vitro. CIF appears to be a non-dialyzable macromolecule which was eluted by Sephadex G200 gel filtration at post-albumin fractions. It can be fractionated by ammonium sulfate precipitation within the range of 60%--70% saturated solution at room temperature. CIF is moderately heat labile and is destroyed by incubation at high pH. CIF is more effective on PE cells from C57 mice than on those from C3H or DBA mice. It also inhibits the clonal growth of committed stem cells for both granulocytes and macrophages present in bone marrow but to a much lesser degree. CIF is not interferon since no antiviral activity is demonstrable in the CIF preparation.

Animals↗
PubMed1981

[Study on anterior-pituitary function during pregnancy and puerperium by radioimmunoassay of human FSH-subunits (author's transl)].

In order to observe the anterior pituitary function of pregnant women in terms of FSH secretion, specific and sensitive radioimmunoassays for human FSH-alpha and -beta subunits were utilized for this study. Blood levels of hFSH-alpha during pregnancy increase acutely and the values of the subunits are as follows: 428.0 +/- 92.64 ng/ml (mean +/- SE., 5-10 weeks of gestation), 962.8 +/- 132.5 ng/ml (11-20 weeks), 1889.0 +/- 137.60 ng/ml (21-30 weeks), 2959 +/- 323.40 ng/ml (31-40 weeks). After child delivery, serum levels of hFSH-alpha decreased to 22.92 ng/ml. In contrast, blood levels of hFSH-beta were not altered significantly through gestation. hFSH-beta subunits: 1.97 +/- 0.74 ng/ml (5-10 weeks of gestation), 2.66 +/- 0.52 ng/ml (11-20 weeks), 2.27 +/- 0.52 ng/ml (21-30 weeks), 2.07 +/- 0.37 ng/ml (31-40 weeks), respectively.

Adult↗
PubMed1980

8-Hydroxyanthracyclinones from epsilon-rhodomycinone.

epsilon-Rhodomycinone was converted into 8,9-dehydro-zeta-rhodomycinone, which gave a cis diol with osmium tetroxide and a pair of epimeric epoxides with m-chloroperbenzoic acid. Acid-catalyzed opening of the epoxides gave the corresponding trans diols. In contrast, acid treatment of the trimethyl ethers of these epoxides gave predominantly a lactone and an eta-rhodomycinone derivative, with only small amounts of the diols. None of the new rhodomycinones were active against Bacillus subtilis, but 8,9-dehydro-zeta-rhodomycinone was active in the induction of lytic phage in Escherichia coli.

Anthracyclines↗