Search PubMed⌕ Search

Biomedical subjects

H Liebermann

Publications and source records attributed to H Liebermann.

At least 91 records · Page 5Linked to original sources
PubMed1978

The central control of shivering and non-shivering thermogenesis in the rat.

1. To test whether the preoptic area controls only non-shivering and the spinal cord only shivering thermogenesis, ten rats were chronically implanted with a preoptic and a spinal cord thermode each. The following were then studied: (a) the effect of propranolol (8 mg/kg.hr) on the metabolic response to cooling the preoptic area, and the spinal cord, (b) the effect of exogenous noradrenaline (0.5 mg/kg) on the metabolic response to cooling the preoptic area, and the spinal cord, and (c) the effect of warming the preoptic area on the metabolic response to cooling the spinal cord, and vice versa. 2. Administration of propranolol inhibited the metabolic response to cooling each of the thermosensitive areas, but the response to cooling the preoptic area was more strongly inhibited than that to cooling the spinal cord. 3. Administration of exogenous noradrenaline did not prevent the metabolic response to cooling either the preoptic area or the spinal cord. 4. Warming the spinal cord completely inhibited the metabolic response to cooling the preoptic area, and warming the preoptic area fully inhibited the metabolic response to cooling the spinal cord. 5. It is concluded that exogenous noradrenaline underestimates the capacity for non-shivering thermogenesis, and that both thermosensitive areas can control both forms of thermogenesis, but that the preoptic area threshold of non-shivering thermogenesis is probably lower than that of shivering, while the spinal cord threshold of shivering is probably lower than that of non-shivering thermogenesis.

Animals↗
PubMed1978

[Some physical properties of the virus of larynxpapillomatosis in human beings (author's transl)].

Cell culture propagated virus of larynxpapillomatosis of men was purified and concentrated. Ultracentrifugation revealed an approximately 130 S- and a circa 214 S-component. 37 nm and 49 nm particles were found by electron microscope technique, the latter showing a bouyant density in CsCl of 1,34 g/ml. These first experiments demonstrate a close resemblance between this virus and polyomaviruses.

Animals↗
PubMed1978

[Ultracentrifugation in virus diagnosis].

Ultracentrifugation has assumed growing importance in virus diagnosis as a technique by which to concentrate and purify viruses for immediacy diagnosis on the basis of electron microscopy as well as for purely virological and serological tests. Ultracentrifugation has proved to be helpful for sizeable improvement of sensitivity for detection, which, in turn, has been conducive to time saving. The preparational ultracentrifuge enables also direct diagnosis by determination of isodensities and sedimentation coefficients of viruses and their components. Examples are mentioned, in that context, such as FMD virus, virus of vesicular swine disease, virus of larynx papillomatosis of man, and goose influenza virus. An account is given of isodensities and sedimentation coefficients of animal viruses.

Animals↗
PubMed1977

[Experimental infection of man using viral strains of bovine papular stomatitis, orf, pseudocowpox and milker's nodule].

An account is given of the close correlations that exist between virus strains of bovine papular stomatitis, orf, pseudocowpox, and milker's nodule. Reference is made to literature data on natural infection of man with the above virus strains. A report then is presented on experimental infection of human volunteers, using paravaccine birus. While fairly tough and elevated nodules, 4 mm to 5 mm in diameter, were produced on the probends' skin, no re-isolation of virus was achieved.

Animals↗
PubMed1976

[Physico-chemical properties of swine vesicular disease virus].

Titration of SVDV on primary pig kidney cell cultures revealed a plating efficiency of less than or equal to 0,9 X 10(-3). Concentration and purification of the SVD-Virus propagated on pig kidney cell cultures were done by chloroform treatment, adsorption, differential- and density gradient centrifugation. The following physical parameters were found: SVDV is an isometrical RNA-virus having a diameter of 25,1 +/- 1,0 nm. It is resistent to the action of chloroform, ether and pH. The virus has a sedimentation coefficient of 156 +/- 3S and a bouyant density in CsCl of 1,33 +/- 0,01 g/ml. Within the family of picornaviruses the SVDV belongs to the subgroup of enteroviruses and can be distinguished from the foot-and-mouth disease virus by the difference in pH-sensitivity and bouyant density in CsCl.

Animals↗
PubMed1975

[Electron microscopy demonstration of fibrillar structures in the foot-and-mouth disease virus].

Purified FMD virus was heat treated and then examined by electron microscopy with the negative contrast technique. Fibrils of varying length and thickness were present; they were not present after ribonuclease treatment. They were similar to the fibrils described for other picornaviruses. Viral RNA was the essential component of these artificially arranged fibrils, which were composed of threads about 10 A wide, separated from one another by a gap of about 10 A.

Aphthovirus↗
PubMed1975

[Structure of the foot-and-mouth disease virus. 2. Various physical and chemical properties of the 12S components].

The 12S units were purified by heat treatment and acidification of purified, highly-concentrated virus, with separation of viral RNA by gel filtration. The following physical parameters were obtained: - partial specific volume 0.737 +/- 0.020 g/cm3; diffusion soefficient D020,W = 3.96 +/- 0.24 F; sedimentation coefficient S020,W = 12.1 +/- 0.5S; molecular weight 283,00 +/- 30,000 Dalton; refraction increment (determined by ultracentrifugation) was dn/dc = 0.189 +/- 0.010 cm3/g for white light of the "HBO 200". Examination of the 12S component by analytical ultracentrifugation and in sucrose and CsCl gradients showed that the product was uniform; density determined in CsCl was 1.302 +/- 0,00 s g/cm3. The ultraviolet absorption spectrum was characteristic of proteins; extinction values at 278 nm in a layer 1 cm thick and 10 mg/ml at pH was 13.9 +/- 1.9, while the ratio E278 nm/E250 = 2.2. Electron microscopy showed that the diameter was 11.0 +/- 1.6 nm. Morphology of 12S unit was discussed.

Aphthovirus↗