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Biomedical subjects

H Lauterbach

Publications and source records attributed to H Lauterbach.

At least 19 recordsLinked to original sources
PubMed1997

Radioimmunodetection of neuroblastoma cells with I-131-radiolabelled antibodies.

The murine anti-neuroblastoma monoclonal antibodies 15/7 and 19/1/4 should be tested for specific radiolocalization of neuroblastoma by immunoscintigraphic imaging of this tumour growing in mice. Radioiodination of both antibodies was done by chloramine-T method resulted in an immunoreactivity of 75%. The calculated specific activity varied from 51.1 to 126.2 kBq/microgram IgG. In each case, about 500 kBq of labeled antibodies were intraperitoneally injected into human neuroblastoma (SK-N-MC, SK-PN-DW and IMR 5) xenografted severe complete immunodeficient (SCID) mice. Whole-body scintigraphy was performed daily by a scintiscanner to localize the tumour site. After last scanning principal organs were removed and their I-131-uptake was determined by measuring the impulse rate. The best scintigrams were done with I-131-19/1/4 at the second day after antibody injection. Radioconjugates were accumulated at highest in the tumour at the third day after application of 15/7 and 19/1/4 with a tumour uptake of 0.4 and 2.2 per cent of injected dose per gram (%ID/g), respectively. The 15/7-moAbs was accumulated approximately 9-fold higher in the SK-N-MC and SK-PN-DW grafts than in principal organs, whereas the tumour/non-tumour-ratio of the 19/1/4 moAb was 3:1. The results indicate the efficacy of these two neuroblastoma antibodies in radiolabelling and their usefulness for tumour imaging of neuroblastoma engrafted SCID-mice.

Animals↗
PubMed1990

Diacetyl-splenopentin (BCH 069) did not affect the concentrations of various hormones in men.

We investigated the effect of BCH 069 (similar structure as thymopentin) on concentrations of hormones of the hypophysis, adrenals, gonads and thyroid glands in men after a therapy period of 4 or 6 weeks. No significant effects of BCH 069 three times weekly 50 mg s.c. for 4 weeks or i.v. for 6 weeks on serum concentrations of prolactin, LH, TSH, cortisol, testosterone, progesterone, estradiol and ETR were observed.

Adult↗
PubMed1990

[Severe oligozoospermia as a sequela to partial obstruction of the seminiferous tubules].

Arguments for the presence of a partial obstruction as cause of severe oligozoospermia may result from following findings. 1. The frequency of normal FSH values in oligozoospermia (44.9%) do not differ significantly from cases with azoospermia (50%). 2. Including a quantitative analysis of testicular morphology 5 out of 63 patients (7.9%) suffering from severe oligozoospermia show a normal spermatogenesis. 3. The frequency of this constellation in our material including severe oligozoospermia, normal testicular volume, normal FSH value, and normal spermatogenesis agree with few findings in the literature. The number of etiological factors of partial obstruction require also invasive diagnostical procedures like vas deferens radiography and surgical exploration.

Adult↗
PubMed1988

[Prolactin stimulation using the metoclopramide test in females taking oral contraceptives].

In 31 patients taking oral contraceptives (o.c.) for a period between 1 year to more than 3 years, basal serum prolactin levels and metoclopramide induced prolactin values were determined 30 and 60 minutes following an i.v. injection of 10 mg of metoclopramide. The basal prolactin levels were elevated in 7 women to more than 1,000 mU l. The 3 groups of patients taking o.c. with different estrogen doses showed higher drug induced increase of their prolactin levels than the controls. These differences were statistically significant between groups I and III and the control group. No differences could be found between the challenged values of the users groups. The prolactin increase challenged with 200 micrograms TRH in 5 women under o.c. was considerably smaller than that observed in the metoclopramide groups, but exceeded the TRH induced levels found in the controls. The significance of these findings is discussed with special reference to the promotion of prolactinomas.

Adult↗
PubMed1987

[Testicular function in obstructive azoospermia].

The normal histological findings of the testicles with simultaneous azoospermia according to the former opinion renders greatly probable an obstruction of the seminal ducts. The reversed case of an ascertained obstruction, however, must not necessarily contain also normal histological structures or normal hormone tests. In the obstruction syndrome in 6 out of 23 cases we find changes in the diameter of the tubule, reductions of the spermatogenesis and changes at the interstice. Imprint-cytologically an increase of pathological spermatides at the cost of normal cell developments is shown. According to this the FSH-values are slightly but statistically significantly increased. LH, PRL and testosterone are found within the normal. For the diagnostic delimitation of the obstruction syndrome from a production azoospermia according to these findings the performance of a biopsy of the testicles with histological evaluation and the determination of the FSH-value. The relatively discrete tubular changes alone cannot be used for the explanation of the discrepancy between anatomical and functional results of the operative reconstruction of the seminal ducts.

Adult↗
PubMed1984

[Testing of the steroid-related suppressive effect on the hypothalamo-hypophyseal system using twofold stimulation].

Intravenous administration of gonadotropin-releasing hormone (GnRH) induces not only a rapid release of pituitary LH and FSH stored, but also stimulates biosynthesis of these hormones by a long acting process. Repeated injection of GnRH after an interval of 90 to 120 minutes evokes an increased response compared with the first period of stimulation. This pattern of reaction is principally maintained on therapy with steroidal contraceptives. The twofold stimulation provides additional information on hypothalamic-pituitary reactivity exceeding the results yielded by the simple GnRH stimulation test. On the contrary examination of prolactin response to twofold TRH-administration gives no increased diagnostic aid.

Contraceptives, Oral, Combined↗
PubMed1983

[Prolactin and male fertility].

On 68 selected patients with disturbances of the potentia generandi et/sive coeundi (25 males with healthy metabolism and 43 males with diabetes) as well as 14 control persons PRL, LH, FSH, testosterone and oestradiol were determined radioimmunologically and the results were ascribed to sexological, clinical, spermatological and testo-histological findings. A statistically secure correlation was found between PRL values and disturbance of the libido as well as the presence of a gynaecomasty. PRL did not correlate with the spermatological variables volume of ejaculate, relative and absolute number of spermatozoa and motility. PRL did also not correlate with the testohistological findings. A relation between PRL and the peptide hormones LH and FSH as well as the steroid hormones testosterone and oestradiol could not be ascertained. Therefore the diagnostic values of a PRL determination is much limited; the pertinency of a hyperprolactinaemia may be increased by the proof of the symptoms reduction of libido and gynaecomasty with simultaneous disturbance of fertility. In our opinion the definition of hyperprolactinaemia needs revision, since at only determination of the basal value of more than 800 mU/l no more frequently pathologically andrological findings are to be observed than below 200 mU/l.

Adult↗
PubMed1982

[The effect of hemoperfusion with XAD-4 resin on biological constituents of the blood].

13 patients who had undergone a haemoperfusion therapy with Amberlite XAD-4 resin (Haemoresin, Braun, F.R.G.) because of acute intoxication were examined for adsorption of biological material from the blood. Treatment lasting on average 4 hours at a blood-circulation rate of 200 ml/min resulted in a 43% reduction of thrombocytes and in changes in the concentrations of proteins, complement factors, triglycerides, aldosterone and testosterone. Low-molecular substances and electrolytes were however, except for sodium, only slightly influenced. Thus this haemoperfusion system is only suitable for shortterm application and cannot be used without substitution for chronic treatment of terminal renal insufficiency.

Adolescent↗
PubMed1982

Influence of hemoperfusion with XAD-4 resin on the concentration of biologic constituents in the blood.

13 patients, who had been subjected to hemoperfusion with Amberlite XAD-4 resin (Haemoresin, Fa. Braun), because of acute intoxications, were examined for the adsorption of bioactive material from the blood. A mean treatment period of four hours at a blood flow rate of 200 ml/min. resulted in a marked thrombocyte decrease by 43% and changes in the concentrations of proteins, complement components C3 and C4 (16-26%) triglycerides (33%), aldosterone (33%) and testosterone (20%). Low molecular weight substances and electrolytes, however, were only slightly or not affected. Thus hemoperfusion system with its poor biocompatibility and inability to remove BUN, electrolytes and water is only suitable for short-term treatment and cannot be used without detailed control of possible side effects and perhaps substitution for treatment of chronic renal or hepatic failure. Hemoperfusion has proved an efficient method of treating various exogenous intoxications. The adsorption of biologically active material from the blood constitutes several side effects which contraindicate long-term treatment, above all of endogenous intoxications such as chronic renal failure and hepatic failure. With the type of adsorber used - various charcoal preparations, resins etc. such as uncoated material or coated with different substances - these side effects vary and have not yet been investigated in a systematic, comparative and comprehensive way. The present investigations are meant to check the adsorption behaviour of Amberlite XAD-4 Resin in vivo towards some selected blood constituents and the influence of hemoperfusion on the concentrations.

Adolescent↗
PubMed1981

[Androgen regulation in patients with diabetic retinopathy].

In 21 male diabetics (juvenile onset diabetes) with diabetic retinopathy and 13 patients without this condition, the HCG and the LH-RH stimulation tests were performed and the results compared to those of 20 persons with normal metabolism and full vision. The findings can be interpreted as hypothalamic hypophysogonadal dysregulation in case of lowered basal testosterone, significantly inverse correlation to the relative responsiveness of Leydig's cells, lacking correlation between LH and testosterone as well as normal LH-RH test. Differences between patients with and without retinopathy were not detectable.

Adult↗
PubMed1980

Induction of pituitary tumours and hyperprolactinemia in female rats by estrogens. The effect of apomorphine, reserpine and L-dopa.

Prolonged estrogen treatment induced an enlargement of the anterior pituitary gland and finally resulted in irreversible pituitary tumours. Using continuous estrogen delivery [subcutaneous (s.c.) cholesterol-estrogen implants], the effects of ethinyl estradiol and the likewise synthetic estrogens STS 153 and STS 456 have been studied with or without concomitant administration of a progestogen without anti-estrogenic activities (STS 557). There was a dose-dependent prolactin cell-stimulating effect of all these estrogens. Ethinyl estradiol (EE2) had the most marked effect followed by STS 456 which coincides with the relative uterotrophic activity found in rats and mice. The elevated serum prolactin levels in rats with an EE2 implant for 6 months could be reduced slightly but not significantly by apomorphine and reserpine at a daily dose of 100 micrograms/animal and 5 micrograms/animal, respectively. Daily administration of 300 micrograms L-dopa led to a slight but insignificant increase of serum prolactin. The high pituitary weight and serum prolactin levels induced by long-term EE2 treatment were partially reversible after implant withdrawal.

Animals↗
PubMed1980

Effects of an intranasally administered low dose progestogen on the pituitary and gonadal functions.

This study was undertaken to investigate possible mechanisms of action of a daily intranasally administered low dose of norethisterone acetate (40 microgram) in regularly menstruating young women. Besides measurement of basal body temperature, serum concentrations of FSH, LH, estradiol and progesterone were determined over one treatment cycle. An endometrial biopsy was taken on day 27 or 28 of the cycle. In spite of a considerable individual variation in the respective values the following effects could be observed: In 8 of 9 subjects the midcycle LH peak was abolished, while basal FSH and LH levels remained within the control range of the follicular and luteal phases. A variable reduction of progesterone levels below the range indicative of a normal luteal phase function suggested a tendency to atypical corpus luteum formation. In 8 of 9 endometrial biopsies, however, secretory transformation could be found.

Administration, Intranasal↗