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Biomedical subjects

H Lang

Publications and source records attributed to H Lang.

At least 19 recordsLinked to original sources
PubMed1992

Coping and adaptation in pancreatectomized patients: a somatopsychic perspective.

The present study describes various strategies of coping with illness as observed in pancreatectomy patients, a group of patients which has not been investigated before in this field of research. Questionnaire data obtained from a sample of n = 134 were analyzed with regard to sociodemographic (age, sex) and medical characteristics (indication for surgery: chronic pancreatitis vs. pancreatic carcinoma; outcome after surgery: diabetes, hypoglycemia, pain). The relationships between ways of coping and several adaptation criteria were investigated. The question is put up to discussion, if it is possible to clearly separate emotion as a way of coping and emotion as an outcome of coping.

Adaptation, Psychological↗
PubMed1992

[The synthesis of collagenous and noncollagenous proteins by cells from the periodontium].

Synthesis of collagenous and noncollagenous proteins by cells originating from the alveolar bone (AB-cells) and from the periodontal ligament (PDL-cells) of 8 minipigs was analyzed in primary cell cultures. Incorporation of 3H-proline into proteins recovered from cell extracts showed, that 13.7% of protein synthesized by AB-cells and 8.0% of protein synthesized by PDL-cells were collagens. In both cell lines relative amounts of collagens synthesized decreased significantly on subculturing (AB-cells: 9.3%, PDL-cells: 6.0%). Preincubation of 2nd subculture AB-cells with beta-glycerophosphate had no significant effect on collagen synthesis. Considering the fact that relative amounts of collagen recovered from cell extracts represent only 10-20% of collagen recovered from whole culture (i.e. cells and culture medium), we suggest that collagen synthesis is an important component of in vitro-protein synthesis of cells originating from the alveolar bone and the periodontal ligament.

Alveolar Process↗
PubMed1991

A simple ELISA procedure for HIV-1 based on the enzyme beta-lactamase.

The enzyme beta-lactamase (penicillin-amido-beta-lactam-hydrolase, EC 3.5.2.6.) has been shown to be suitable for use in ELISA procedures. The assay proposed requires a conjugate containing a beta-lactam-hydrolase as the enzymatic component, penicillin or one of its derivatives as substrate and an iodic complex (an iodine-starch or an iodine-polyvinylic alcohol complex) as the chromogenic component. Binding of conjugate molecules results in the transition of the iodic complex from dark blue to colourless. The transition is readily visualized without the aid of an EIA reader. The decolorization occurs specifically giving a clear-cut yes/no decision without a cut-off value. The rate of the colour transition strongly depends on the amount of bound conjugate. beta-lactamase-based ELISA techniques are of potential use in the immunological diagnosis of some virus diseases. The sensitivity and specificity of the assay were assessed on a panel of 670 negative and 141 positive HIV-1 sera, giving values of 100% and 99.85%, respectively.

Acquired Immunodeficiency Syndrome↗
PubMed1991

Rat liver dimethylglycine dehydrogenase. Flavinylation of the enzyme in hepatocytes in primary culture and characterization of a cDNA clone.

Dimethylglycine dehydrogenase (Me2GlyDH), an enzyme of choline catabolism specifically expressed in the mammalian liver, was analyzed in rat hepatocytes in culture. This mitochondrial enzyme carries the FAD cofactor covalently attached to the polypeptide chain by its riboflavin 8 alpha position to N pi of histidine [Cook, R., Misono, K.S. & Wagner, C. (1980) J. Biol. Chem. 259, 12475-12480]. Subcellular fractionation of [14C]riboflavin-labelled hepatocytes and immunoprecipitation with Me2GlyDH-specific antiserum identified a [14C]riboflavin-labelled polypeptide of the size of mature Me2GlyDH only in the mitochondrial fraction. Immunoprecipitation of extracts from [35S]Met-labelled hepatocytes revealed a putative precursor protein to the mature Me2GlyDH in the cytoplasmic fraction. These Me2GlyDH polypeptides were not expressed in cells of the rat hepatoma cell line FAO. A Me2GlyDH cDNA clone of apparent full length was isolated from a rat liver cDNA bank constructed in the plasmid vector pcD-X [Okayama, H., Kawaichi, M., Brownstein, M., Lee, F., Yokota, T. & Arai, K. (1987) Methods Enzymol. 154, 3-28]. The nucleotide sequence of the cDNA contains an open reading frame encoding a protein of 96059 Da. This molecular mass agrees well with the migration on SDS/PAGE of the assumed Me2GlyDH precursor immunoprecipitated from the cytoplasm of [35S]Met-labelled cells. Proteolytic cleavage at the putative mitochondrial processing protease-recognition site Arg(-2)-Ala(-1)-Glu(+1) would lead to the formation of a protein of 91391 Da, which is in good agreement with the estimated 90 kDa of mature Me2GlyDH [Wittwer, A.J. & Wagner, C. (1981) J. Biol. Chem. 256, 4102-4108], and a 43-amino-acid leader peptide. The N-terminus of Me2GlyDH contains a conserved amino acid sequence which forms the dinucleotide-binding site in many enzymes with noncovalently bound FAD. Close to the modified histidine there is an amino acid sequence resembling a sequence conserved in thymidylate synthases and shown in these enzymes to be involved in the binding of the pteroyl polyglutamate cofactor.

Amino Acid Sequence↗
PubMed1991

A comparison between morphine, meperidine and ketobemidone in continuous intravenous infusion for postoperative relief.

Morphine, meperidine and ketobemidone used in continuous i.v. infusion for postoperative pain relief were compared in a double-blind, controlled, prospective study in 81 consecutive consenting adult patients after open-heart surgery, with permission from the hospital ethics committee. During the first postoperative period, the infusion rates were fixed. Later on, when the infusion rate could be regulated according to individual patient needs, the variation in infusion rate was large, in accordance with earlier studies. No significant differences were demonstrated between the three analgesics with respect to efficacy of analgesia or side effects like shivering, nausea or vomiting. Respiratory depression following extubation was not observed. During shivering, there was a significant increase in the arterio-mixed venous difference of oxygen in all groups. The amounts of opioids used were relatively small compared to amounts used in patients following abdominal surgery. When interviewed some days after surgery, 18/74 patients remembered moderate pain and 11 severe pain during the stay in the ICU.

Double-Blind Method↗
PubMed1991

The results of a large multicentre study comparing low-dose lisinopril-hydrochlorothiazide with the monocomponents.

The efficacy, tolerability and metabolic effects of a fixed combination of lisinopril 10 mg and hydrochlorothiazide 12.5 mg (L/HCTZ) and those of the monocomponents were compared in 286 mild to moderate hypertensive patients in a 17-centre study using a double-blind, randomized, parallel-group design. After 2 weeks of placebo run-in, patients were randomized to one of the three treatments for 8 weeks. Supine and standing BPs were measured at peak blood levels (4-8 hours post dose) after 4 weeks, and at trough blood levels (24-28 hours post dose) after 8 weeks. The BP reduction from baseline was greater with L/HCTZ than with the monocomponents at weeks 4 and 8; at week 8 the reduction was statistically significant vs lisinopril for standing DBP (P less than 0.027) but not for standing SBP; the reduction was significant with L/HCTZ vs hydrochlorothiazide at week 4 [P = 0.0002/0.0001 (SBP/DBP) supine; P = 0.0002/0.0002 standing] and at week 8 (P = 0.0001/0.0001 supine and standing). Response at week 8 (supine DBP less than or equal to 90 mm Hg) with L/HCTZ (87.0%) was greater than with hydrochlorothiazide (29.0%; P less than 0.0001) and marginally greater than with lisinopril (74.2%; P = 0.069). Heart rate fell statistically significantly more with L/HCTZ (by 3.3 beats/minute) than with HCTZ (by 0.86 beats/minute; P = 0.018). L/HCTZ treatment significantly increased plasma uric acid levels compared with lisinopril. L/HCTZ decreased potassium, while lisinopril increased potassium. L/HCTZ decreased cholesterol; HCTZ increased it. Adverse effects were more common on L/HCTZ vs lisinopril (P = 0.033) but not vs hydrochlorothiazide.(ABSTRACT TRUNCATED AT 250 WORDS)

Adult↗
PubMed1991

[Neuropathy of the gingival autonomous nerve system in inflammatory diseases of the periodontium. Ultrastructural analysis].

In a comparative study the influence of periodontal disease on the autonomous nerve-system of human attached gingiva was analysed ultrastructurally in 20 gingiva specimens. The test group was formed by 10 patients with adult periodontitis (AP) and compared with 10 patients with clinically healthy gingiva. The specimens were prepared for ultrastructural evaluation. The autonomous nerve fibres near the epithelium, in the connective tissue, and at the blood vessels were qualified and quantified by means of electron microscopy. In the test group clear signs of neuropathy of the autonomous nerve system like lysis of axoplasm, evagination of axons from their Schwann's sheaths, de- and regeneration pistons and reduction of axons were found. The possible pathogenic significance of this neuropathy of the autonomous nerve system in periodontal disease is discussed.

Adult↗
PubMed1991

[Measurement of O2 concentration in the fresh gas supply of the Kuhn system].

To accomplish the requirement of inspiratory O2-concentration measurement on the Ayre-T-piece modified by Kuhn we developed a special device. It is mounted in the fresh gas hose and then connected to the sensor of the O2-concentration measuring and warning instrument of the circle system.

Anesthesia, Inhalation↗
PubMed1990

The use of cultures of human osteoblastlike cells as an in vitro test system for dental materials.

An in vitro culture of human osteoblastlike cells was established in order to perform toxicity tests with materials interacting with bone in vivo. Different dental materials were tested with osteoblastlike cells and with human gingival fibroblasts. Direct and indirect tests were performed according to the different properties of the materials. It was found that the osteoblastlike cells in test cultures were affected by materials that caused no visible cell damage in the fibroblast cultures. The results indicated that human osteoblastlike cell cultures represent a highly sensitive test system for cell-specific toxicity.

Cells, Cultured↗
PubMed1990

Alpha rhythm in classical migraine (migraine with aura): abnormalities in the headache-free interval.

The alpha rhythm of 18 patients with classical migraine (migraine with aura) was studied by EEG spectrum analysis for evidence of neural abnormalities during the asymptomatic period. The temporal relationship of the findings to attacks was studied by serial records in 11 cases. Increased frequency dispersion and frequency asymmetries of the alpha rhythm were found. The records were, however, mostly normal when separated from attacks by at least 10 asymptomatic days. The abnormalities increased significantly before the onset of prodromal symptoms and clearly outlasted the headache phase. The results give evidence of a fluctuating asymmetric neural disorder in classical migraine.

Adult↗
PubMed1990

[Is preservation of erythrocyte concentrates with added solutions an alternative to deep freezing in heart surgery autologous blood donation?].

In patients undergoing cardiovascular operations the deep freezing of autologous red blood cells (RBC) was compared with the liquid storage in PAGGS-Sorbitol. On an average 3.9 units of blood were predeposited in the group with deep freezing and 3.4 in the group with liquid storage. The number of complications was the same in both groups despite the shorter intervals between the donations in the group with liquid storage. 58 out of 74 patients (= 78%) with deep frozen autologous RBC and 42 out of 65 patients (= 65%) with liquid stored RBC need no homologous blood. 12% of the liquid stored RBC were discared due to unforseen delaying of the operation.

Adenine↗
PubMed1989

Re-implantation of homologous, cultivated osteoblast-like cells for improvement of bone regeneration. An animal study.

The regeneration of osseous tissues after implantation of rat osteoblast-like cells cultivated in vitro was investigated. A cell line of rat osteoblast-like cells originating from explants of four inbred rats (Lewis) was established. The osteoblast-like cells were cultivated in vitro and re-implanted after 3 passages in cell culture into artificial femur defects of the same inbred Lewis rats. The cultivated osteoblast-like cells were embedded in bovine bone gelatine and implanted together with hydroxylapatite granulate (12 animals), pyrolized calf bone (12 animals) or without any additional material (12 animals). These bone replacement materials were used as a carrier for the osteoblast-like cells. After 10, 20, 40 and 80 days animals were killed and the treated femora were taken for histological examination and determination of bone growth rate. It was demonstrated that the cultivated osteoblast-like cells in this experiment had no measurable effect on quantitative bone growth, but caused a significant difference in the histological bone regeneration pattern.

Animals↗