Search PubMed⌕ Search

Biomedical subjects

H Lal

Publications and source records attributed to H Lal.

299 records · Page 17Linked to original sources
PubMedDate not supplied

Pathogenesis of Alzheimer's disease: current status on apolipoprotein E4 gene research.

Alzheimer's disease (AD) affects millions of people throughout the world. The financial and emotional costs that are inflicted by this devastating disease are enormous. Genes responsible for the early-onset familial AD have been cloned and found to be membrane associated transport proteins. Evidence suggests that apolipoprotein E4 may be the risk factor for the late-onset form of the disease. Beta-amyloid and potassium channel dysfunction have also been implicated in the development of AD. Hyperphosphorylation of "tau" has been indicated for the generation of neurofibrillary tangles in the brains of Alzheimer's patients. Since apoE4 does not bind to "tau", apoE4 may contribute to the hyperphosphorylation of "tau" which may cause the formation of neurofibrillary tangles in AD patients. Molecular biology research on AD should help in the development of drugs for the prevention and treatment of the disease.

Age of Onset↗
PubMedDate not supplied

DOTS centre at a tertiary care teaching hospital: lessons learned and future directions.

BACKGROUND: In 1993, Government of India started the Revised National Tuberculosis Control Programme (RNTCP). A model Directly Observed Therapy, Short-Course (DOTS) centre was established at the All India Institute of Medical Sciences (AIIMS) to (i) identify the challenges and opportunities in establishing DOTS centres at tertiary care facilities, (ii) to teach the strategies of RNTCP to medical and paramedical staff, and (iii) to undertake relevant operational research connected with tuberculosis (TB) treatment and control. In this communication, we describe the experience of establishing a DOTS centre at India's premier medical institute and discuss the lessons learned. METHODS: Since September 2001 through November 2002 AIIMS employees and their dependants diagnosed with tuberculosis were enrolled for treatment at AIIMS DOTS centre. One hundred sixty-eight patients were diagnosed as suffering from tuberculosis. Of these 49 patients were referred out and remaining 119 patients were treated at AIIMS DOTS centre. RESULTS: Treatment success was achieved in 80% (20/25) of new smear positive cases and the DOTS centre achieved other targets set up by the RNTCP. As the results of the pilot study at AIIMS DOTS centre were favourable, the facilities of AIIMS DOTS centre were extended to the general public from September 2002 onwards. CONCLUSION: Despite tremendous patient load at tertiary care facilities, it is possible to achieve targets established by the RNTCP. However, additional research needs to be conducted especially relating to drug resistance and surrogate markers of failure under RNTCP.

Adult↗
PubMedDate not supplied

Gamma-glutamyl transpeptidase in leprosy.

Activity of the enzyme-gamma-glutamyl transpeptidase (GGTP) was measured in sera of 20 patients each of paucibacillary and multibacillary leprosy and 20 healthy controls. None of the subjects had any systemic or hepatic disease and none had taken any hepatotoxic or antileprotic drugs in the past 3 months. Mean values in the paucibacillary group (38.62 +/- 1.99 U/L) and in the multibacillary group (59.04 +/- 3.13 U/L) were significantly higher compared to that in controls (32.04 +/- 0.66 U/L). Mean value in the multibacillary group was also significantly higher compared to that in the paucibacillary group.

Adolescent↗
PubMedDate not supplied

Effect of dietary restriction with and without leucine supplementation on hepatic protein status in rats.

In the present study effect of dietary restriction with and without leucine supplementation was observed on body and liver weights, and liver protein status, in adult rats. Animals were fed on two diets ad lib or were on 50 per cent and 25 per cent intakes. Dietary restriction resulted in loss of body and liver weights, hepatic protein, free-alpha-amino nitrogen and RNA contents and liver cell size (liver weight/DNA ratio). When compared with the control group, the decrease in these parameters was more in the dietary restricted leucine supplemented group. However, hepatic DNA content was not changed with the change in dietary regimen. The results suggest that leucine supplementation with dietary restriction may be more harmful for the animal than dietary restriction alone.

Animals↗
PubMedDate not supplied

Effect of acute environmental heat stress on urinary water and electrolyte excretion in the rat.

Plasma cortisol and urinary excretion of water, sodium, potassium, calcium and magnesium have been studied in the rat after application of heat stress. There was a significant increase in plasma cortisol level after exposure to heat. During heat stress complete cessation of urine formation was observed. In the next 30 min there was statistically significant increase in the urinary excretion of water, sodium and calcium but not of potassium and magnesium. Urinary calcium/magnesium ratio was also significantly elevated. The increase in urinary water and electrolyte excretion seemed to be mediated through prostaglandins since it could be abolished by administration of indomethacin prior to the application of heat stress. On the basis of these results, the possible role of heat stress in the genesis of urolithiasis has been discussed.

Animals↗
PubMedDate not supplied

Acute effects of neurogenic stress on urinary electrolyte excretion.

Plasma Cortisol and urinary excretion of water, sodium, potassium, calcium and magnesium have been studied in the rat after application of 2 types of neurogenic stress:--(a) tight rubber band tourniquet and (b) electric shock. Plasma cortisol levels increased significantly after application of either type of stress. During both type of stress, there was statistically significant increase in the urinary excretion of water, sodium and calcium but not of potassium and magnesium. Urinary calcium/magnesium ratio was also significantly elevated. The results suggest that stress may be one of the factors involved in the genesis of urolithiasis.

Animals↗
PubMedDate not supplied

Effect of prolonged starvation and refeeding on fuel metabolism in rats.

Body and liver weights, Liver lipids, glycogen, aspartate aminotransferase (EC 2.6.1.1), alanine aminotransferase (EC 2.6.1.2) and blood glucose levels were determined in starved and starved-refed rats. Decrease in body and liver weights was rapid during the initial stage of starvation and slowed down thereafter. Water was the major liver constituent lost in early fast. Following 10 days of starvation, body weight was reduced by nearly 20%, liver weight 43%, liver glycogen 93% and blood glucose 34%. Liver lipids and the activities of the two transaminases however, were increased by about 30-50%. On refeeding body weight and its water content increased and became nearly double of the initial fasting value on day 2. Blood glucose, liver glycogen, liver lipids and transaminases were significantly altered and got normalised within 5-8 days.

Alanine Transaminase↗
PubMedDate not supplied

Detection of antibodies to phenolic glycolipid by ELISA in leprosy patients.

Antibody (IgM) response to PGL-1, a surface glycolipid unique to Mycobacterium leprae has been studied in 25 cases each of lepromatous and tuberculoid leprosy and in 25 healthy controls. The absorbance value at 488 nm was expressed as antibody titre. Serum antibody titre was found to be significantly higher in patients than controls. Results confirm that antibody response in leprosy patients depend upon bacterial load.

Antibodies, Bacterial↗