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Biomedical subjects

H L Vis

Publications and source records attributed to H L Vis.

At least 37 records · Page 2Linked to original sources
PubMed1984

Abnormal fatty acid turnover in the phospholipids of the red blood cell membranes of cystic fibrosis patients (in vitro study).

A study was carried out in order to investigate whether the abnormal in vitro turnover of fatty acids in the phospholipids of the red blood cell membranes of cystic fibrosis patients is intrinsic to the membrane, or whether it is induced by extrinsic serum factors. Red blood cells of cystic fibrosis patients and healthy subjects were labeled in vitro with [14C]linoleic acid, bound to albumin. The labeled cells were reincubated in autologous and homologous serum. The radioactivity present in the serum lipids and in the major phospholipid fractions of the red cell membranes was measured. Conclusions of this study are: 1) not all of the cystic fibrosis patients examined individually show an abnormal in vitro turnover of the red cell fatty acids, although they all presented abnormal fatty acid patterns for the red blood cell phospholipids, the platelet phospholipids, and the plasma lipids. 2) The in vitro abnormal fatty acid turnover occurs only in the incubations where red cells of cystic fibrosis patients are involved (in homologous and autologous serum) and not where red cells of healthy subjects are incubated in serum of cystic fibrosis patients. Consequently, the abnormal turnover is intrinsic to the red cells and is not induced by extrinsic serum factors.

Adolescent↗
PubMed1983

Enteropathogenic agents in children with diarrhoea in rural Zaire.

A systematic study of enteropathogenic agents in the stools of children was carried out in a rural area of Kivu Province in Zaire in June, 1979. 84 inpatients and 271 outpatients with diarrhoea were investigated together with 117 inpatient and 203 outpatient controls without diarrhoea. Campylobacter jejuni was the most frequently recovered pathogen in both inpatients (24%) and outpatients (13.7%). Enterotoxigenic Escherichia coli was the next most common among children presenting as outpatients with diarrhoea (10.3%). Yersinia enterocolitica was isolated for the first time in this region. Rotaviruses were found only in outpatients with diarrhoea, and usually in children under two years of age. C. jejuni and Vibrio cholerae could also be isolated from the faeces of domestic animals living in close contact with these village families. Analysis of the clinical features did not lead to the recognition of a pattern typical of any particular pathogen. Salmonella, Shigella, and enteropathogenic E. coli did not play a significant role in the cases studied.

Bacterial Infections↗
PubMed1982

Plasma amino acid patterns, one and two hours after continuous naso-gastric alimentation of low birth weight infants fed two types of milk during the first month of life.

Plasma amino acid concentrations were determined in the morning, 1 and 2 h after discontinuing any naso-gastric feeding on the 3rd, 5th, 15th and 30th days of life, in order to establish their course according to the moment of sampling and to the type of diet given. Two types of conventional naso-gastric feeding were given in 12 infants with a birth weight of 1,500 g or less. The diets supplied either 1.2 g proteins/100 ml (pooled human milk) or 1.8 g proteins/100 ml ("humanized formula' with an albumin/casein ratio of 60/40) from the 3rd h to the 30th day of life. No statistically significant difference appeared between the amino acid concentrations 1 and 2h after discontinuation of naso-gastric alimentation on the 3rd, 5th and 15th days whichever milk was used, but a significant difference appeared for two amino acids on day 30 (phenylalanine and lysine) when fed the humanized formula. It is concluded that caution might be necessary when interpreting amino acid results: the moment of sampling induced a statistically significant difference on day 30 of life only for phenylalanine and lysine in children fed on a "humanized formula'.

Amino Acids↗
PubMed1982

In vitro study of the incorporation and transport of nonesterified fatty acids into the phospholipids of the red blood cell membranes of cystic fibrosis patients.

The in vitro incorporation and transport of plasma nonesterified fatty acids into phospholipids of red cell membranes have been studied in cystic fibrosis and healthy children. Red blood cells were labeled in vitro by an active "acyltransferase"-dependent incorporation of radioactively labeled nonesterified fatty acids. [3H]-Palmitic and [14C]-linoleic acid, bound to albumin, have been studied simultaneously because it has been shown before that the concentration of palmitic acid increases and the concentration of linoleic acid decreases both in the plasma nonesterified fatty acid fraction and in the various phospholipids of the erythrocyte membranes of cystic fibrosis patients. The labeled cells were reincubated in autologous serum and the radioactivity present in the serum lipids and in the major phospholipid fractions of the erythrocyte membranes was measured. A general conclusion is that the in vitro turnover of labeled palmitic and linoleic acids in the phospholipids of the erythrocyte membranes is higher for cystic fibrosis patients than for healthy children. No difference is detectable between the in vitro behaviour of [14C]-linoleic versus [3H]-palmitic acid in cystic fibrosis patients compared with healthy children.

Biological Transport↗
PubMed1980

Altered phospholipid composition and changed fatty acid pattern of the various phospholipid fractions of red cell membranes of cystic fibrosis children with pancreatic insufficiency.

After two-dimensional thin-layer chromatography, it appears that the phospholipid composition of the erythrocyte membranes of cystic fibrosis children, compared with healthy ones, is changed by an increase of the phosphatidyl inositol content. The plasmalogen concentration of the membrane phospholipids is the same for both groups. The fatty acid pattern of the various red cell membrane phospholipid fractions was determined by gas chromatography (Tables II, III and IV), and significant changes were observed in the cystic fibrosis patients (Table V). Phosphatidyl choline and ethanolamine (non-plasmalogen forms) especially show strikingly abnormal fatty acid patterns similar to those in the various plasma lipid fractions.

Child↗
PubMed1980

Long chain non-esterified fatty acid pattern in plasma of cystic fibrosis patients and their parents.

The absolute and the percentage plasma NEFA pattern of healthy children, Cystic Fibrosis (C.F.) patients and their parents have been determined (table I, III, V) and compared with those of age and sex matched control subjects (table II, Iv, V). There is a striking difference in the percentage plasma NEFA pattern of C.F. patients with pancreatic insufficiency: palmitic, palmitoleic and oleic acid are significantly increased while linoleic, linolenic and stearic acid are decreased (table IV). Four C.F. children without pancreatic involvement have the same abnormal NEFA pattern (fig. 3). The overlapping areas of the distributions of both palmitoleic and linoleic acid (as shown in fig. 2a and B) are small for healthy and C.F. subjects. About 90% of the C.F. subjects examined show an abnormal palmitoleic and/or linoleic acid concentration. Tested individually, recognition of this typical C.F. pattern involves a possible error of 15% (fig. 3). A correlation exists between the abnormality of the plasma NEFA pattern in C.F. patients and the severity of pulmonary disease. The percentage plasma NEFA pattern of C.F. parents is modified by an increase of linoleic acid and a decrease of saturated fatty acids (table V, fig. 1a, B). This is possibly due to an higher dietary intake of polyunsaturated fatty acids.

Adolescent↗