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H L Moore

Publications and source records attributed to H L Moore.

At least 19 recordsLinked to original sources
PubMed2001

Effect of cause and time of dropout on the residual GFR: a comparative analysis of the decline of GFR on dialysis.

BACKGROUND: The decline of residual renal function (RRF) on dialysis has been reported to be slower in peritoneal dialysis (PD) then hemodialysis (HD). However, some clinicians have questioned whether this reported difference might not be caused by selection bias. In particular, if continuous ambulatory PD (CAPD) delivers only marginally adequate therapy as some clinicians speculate, then perhaps those patients on CAPD with low glomerular filtration rate (GFR) are purposefully switched to HD. If true, transferring CAPD patients with low GFR to HD could create a selection bias that very well may account for the differences in GFR between PD and HD. This is particularly problematic if one then censors patients at the time of transfer from PD to HD from analysis (that is, patients are no longer followed in the study once they have switched treatment modalities). When this occurs, the data are said to be informatively censored, a term used by statisticians to describe any kind of systematic bias associated with censored or incomplete data. In particular, informative censoring occurs when patients who die or transfer to another modality very early have an associated lower starting GFR or higher rate of decline of GFR than patients who either complete the study or who die or transfer much later. If patient dropout is indeed related to the rate of decline in GFR and if this relationship differs between PD and HD but is ignored in the analysis, then the results of such analysis may be biased. METHODS: This article analyzes the decline in GFR among 141 incident dialysis patients (39 HD and 102 PD) undergoing either HD or PD at the University of Missouri-Columbia. The decline in GFR was modeled as a nonlinear function of time, taking into account the possibility that missing values of GFR may be associated with patient dropout (death, transfer to another modality, or transplantation). To safeguard against this possibility, we utilized a conditional nonlinear mixed-effects model. The model was used to fit and compare each patient's GFR data to time adjusting for the patient's treatment modality (HD vs. PD), cause of dropout (death, transfer, transplant, lost to follow-up/study ended), and time to dropout. The model allowed a comparison of the starting GFR and the rate of decline in GFR between PD and HD adjusting for these three factors. RESULTS AND CONCLUSIONS: The results of our analysis suggest that such informative censoring is independent of treatment modality and that even after correcting for dropout caused by death or transfer to another modality, patients starting on PD have a lower rate of decline in GFR (that is, better preservation of GFR) than patients starting on HD.

Aged↗
PubMed2001

Chronic peritoneal dialysis in iron-deficient rats with solutions containing iron dextran.

BACKGROUND: We evaluated the effects of different concentrations of iron dextran administered through the intraperitoneal route, in iron-deficient rats, on hematocrit (Hct in percentage), serum iron (mg/dL), total iron binding capacity (TIBC in mg/dL), and the function and histology of the peritoneal membrane. METHODS: Seventy-two male Sprague-Dawley rats weighing 85 to 110 g were divided into two groups and seven subgroups. Group I consisted of rats on iron-deficient chow, and group II consisted of rats on normal chow. Both groups contained dialysis control subgroups (N = 12: IA, IID), dialyzed with Dianeal solution, and tissue control subgroups (N = 6: IE, IIN), in which rats were not dialyzed and catheters were not implanted. Study group I contained the following study subgroups (N = 12): (B) rats dialyzed with Dianeal solution containing 2 mg/L of iron dextran and (C) rats dialyzed with Dianeal solution containing 1 mg/L of iron dextran. Group IID was dialyzed with Dianeal solution containing 2 mg/dL of iron dextran. Study duration was 12 weeks with peritoneal equilibration tests (PETs) performed at baseline, 6 weeks, and 12 weeks. Prior to baseline, rats were placed on iron-deficient chow or normal chow for three weeks. Dialysis was performed with three 25 mL volume exchanges per day. Hematocrit (Hct), serum iron (Fe), and total iron binding capacity (TIBC) were determined for each study interval. After the final PET, the animals were sacrificed, and the peritoneal membrane was evaluated by gross inspection and light microscopy. RESULTS: Rats on an iron-deficient diet developed severe iron-deficiency anemia after three weeks of the diet (Hct 27; Fe 21 to 23; TIBC 799 to 806). After 12 weeks, the rats remained anemic in groups A (Hct 34 +/- 0.9; Fe 16 +/- 2; TIBC 998 +/- 27) and IE (Hct 38 +/- 2.7), whereas the rats corrected anemia in group B (Hct 45.8 +/- 1.8; Fe 115 +/- 15; TIBC 546 +/- 77). The results were not significantly different from those of group IID (Hct 47.1 +/- 1.6; Fe 94 +/- 19; TIBC 516 +/- 46). In group C, Hct (44.8 +/- 2.1) and Fe (94 +/- 19) did not differ significantly from group IID, but TIBC (734 +/- 76) remained significantly higher than that in the group IID. Peritoneal iron deposits were not detected. The morphometric analysis of the submesothelial space did not reveal any difference in thickness between dialysis groups. PETs were not significantly different among groups. CONCLUSIONS: Intraperitoneal iron dextran supplementation in concentrations of 2 mg/L of dialysis solution is nontoxic to the peritoneum and effective in correcting iron deficiency in rats maintained on an iron-deficient diet. Iron dextran in concentration of 1 mg/L of dialysis solution may be sufficient for correcting a lesser degree of iron deficiency.

Animals↗
PubMed2001

Determination and importance of clinical and patient-based measures in outcome assessment of peripheral arterial occlusive disease.

Therapeutic effectiveness is the overall effect of an intervention on clinical and quality-of-life measures. Traditionally, in peripheral arterial disease, this has been evaluated in terms of clinical outcomes only. The lack of correlation between quality-of-life and clinical measures means that these cannot adequately describe overall patient benefit or adverse effects from an intervention. Therefore, patient-based measures such as changes in disease-specific questionnaire scores must be included in the evaluation of therapeutic effectiveness.

Arterial Occlusive Diseases↗
PubMed2000

Preservation of glomerular filtration rate on dialysis when adjusted for patient dropout.

BACKGROUND: Residual renal function (RRF) plays an important role in dialysis patients. Studies in patients on maintenance dialysis suggest that RRF is better preserved in patients receiving peritoneal dialysis (PD) vis-à-vis those receiving hemodialysis (HD). We speculated that regardless of the patient's type of therapy, the estimate obtained for the rate of decline in glomerular filtration rate (GFR) may be biased because of informative censoring associated with patient dropout. Informative censoring occurs when patients who die or transfer to another modality very early have associated with them a lower starting GFR or a higher rate of decline of GFR than patients who either complete the study or who die or transfer much later. If patient dropout is indeed related to the rate of decline in GFR and if this relationship is ignored in the analysis, then the estimate obtained of the rate of decline in GFR may be biased. METHODS: In an attempt to determine if there is a relationship between patient dropout and the decline in GFR, we reanalyzed the CANUSA data by modeling GFR as a nonlinear function of time with the rate of decline being exponential. RESULTS: This article highlights the significance of "informative censoring" when studying the decline of RRF on dialysis. The results show that for the CANUSA cohort, the mean initial GFR was significantly lower, and the rate of decline was significantly higher for patients who died or transferred to HD than for patients who were randomly censored or received a transplant. It is important to emphasize that the impact of informative censoring on previous analyses of the decline of RRF between PD versus HD is presently unclear. If bias caused by informative censoring is the same regardless of what therapy a patient is on, then conclusions from previous studies comparing the decline in GFR between PD and HD would still be valid. However, if the magnitude of the bias differs according to therapy, then additional adjustments would be needed to fairly compare the decline in GFR between PD and HD. Because this analysis is restricted to patients on PD, it would be scientifically incorrect to interpret previous studies solely on the basis of the results from this analysis. CONCLUSION: In any longitudinal study designed to estimate trends in an outcome measured over time, it is important that the analysis of the data takes into account any effect patient dropout may have on the estimated trend. This analysis demonstrates that among PD patients, both the starting GFR and the rate of decline in GFR are associated with patient dropout. Consequently, future studies aimed at estimating the rate of decline in GFR among PD patients should also account for any dependencies between dropout and GFR. Similarly, data analyzing for apparent differences in the rate of decline of GFR between PD and HD should also adjust for possible informative censoring.

Glomerular Filtration Rate↗
PubMed2000

Peritoneal accumulation of advanced glycosylation end-products in diabetic rats on dialysis with icodextrin.

OBJECTIVE: To evaluate and compare the effects of glucose-based solutions to those of icodextrin with respect to peritoneal transport characteristics and formation of advanced glycosylation end-products (AGEs) in the peritoneal membrane in the diabetic rat model of peritoneal dialysis (PD). STUDY DESIGN: Thirty-three male Sprague-Dawley rats weighing between 275 - 300 g were divided into 5 groups: group C (n = 6), control rats with catheter but not dialyzed; group D (n = 5), diabetic rats with catheter but not dialyzed; group G (n = 7), diabetic rats dialyzed with standard 2.5% glucose solution for daytime exchanges and 4.25% glucose solution for the overnight exchange; group H (n = 8), diabetic rats dialyzed with standard 2.5% glucose solution for daytime exchanges and 7.5% icodextrin solution for overnight exchanges; group I (n = 7), diabetic rats dialyzed with 7.5% icodextrin solution for all exchanges. Dialysis exchanges were performed three times daily with an instillation volume of 25 mL per exchange for a period of 12 weeks. Tissue sections were stained using a monoclonal anti-AGE antibody. One-hour peritoneal equilibration tests (PET) were performed every 4 weeks for comparison of transport characteristics. RESULTS: The level of immunostaining was lowest in group C and highest in group G. Significant differences were seen between group C and groups G, H, and I (p < 0.001, p = 0.001, and p< 0.05 respectively). Significant differences were also found between group G and groups D and I (p < 0.05 and p < 0.05 respectively). Over time, glucose concentration at the end of an exchange versus concentration at instillation (D/D0 glucose) decreased and dialysate-to-plasma ratio (D/P) of urea increased. Significant differences were found between groups C and H for D/D0 glucose (0.40+/-0.01 vs 0.35+/-0.01, p < 0.05); and between groups C and H for D/P urea (0.87+/-0.03 vs 0.97+/-0.02, p < 0.05). CONCLUSIONS: These results suggest that AGE formation is lower with the use of peritoneal dialysis solution containing icodextrin than with glucose-based solutions. We conclude that the use of icodextrin may be helpful in slowing the deterioration of the peritoneal membrane, prolonging its use for dialysis.

Animals↗
PubMed1999

Effects of chlorpromazine or diltiazem given intraperitoneally alone or in combination on peritoneal transport of solute and water.

Calcium channel blocker given intraperitoneally (i.p.) in rats was reported to increase urea D/P ratio without protein loss. Chlorpromazine (CP) given i.p. in humans was reported to increase ultrafiltration (UF) and urea clearance. We studied the effects of i.p. Diltiazem (DZ) (15 mg/kg) and i.p. chlorpromazine (0.25 mg/L dialysate)--given alone or in combination--on urea D/P ratio, dialysate protein (Dpro), glucose concentration (Dg), UF, and drainage volume (Vd). Six male Sprague-Dawley rats were studied. The rats underwent 21 consecutive 30-minute exchanges with 15 mL of 1.5% of Dianeal solution (Baxter Healthcare Inc., Deerfield, Illinois, U.S.A.). DZ or CP was added to the dialysis solution during exchanges 4-6 and 10-12. During exchange 16-18 both DZ and CP were added to the dialysis solution. Exchanges 1-3, 7-9, 13-15, and 19-21 were control exchanges performed with 1.5% Dianeal solution alone. The mean weight of the rats was 541.6 +/- 44 g. The animals' blood pressure remained stable during the study period. An increase in D/Purea ratio was observed with DZ, with CP, and with the two drugs in combination, without increase in dialysate protein loss. An increase in UF with a decrease in D/D0 was observed with DZ, with CP, and with the two drugs in combination, suggesting a mechanism other than osmotic gradient--such as increased blood flow or decreased surface tension.

Animals↗
PubMed1998

Seven spoonfuls of preventive medicine for sexual harassment in health care.

Sexual harassment claims have increased substantially since 1990 and continue to be prominent in the Equal Employment Opportunity Commission's discrimination caseload. The authors surveyed high-level training and human resource practitioners in small, medium, and large health care organizations for suggestions to counter this trend. Three fourths of these professionals suggested that behavior modeling of strong policies combined with effective training helped. The survey results suggest seven preventive medicine strategies for reducing work-related sexual harassment incidents in health care organizations.

Adult↗
PubMed1996

Effects of bicarbonate dialysis solution on peritoneal transport in rats.

We studied the effects of bicarbonate dialysis solution (TB 1.36) on the peritoneum in our rat model of dialysis. Twenty-four male Sprague-Dawley rats were divided into two groups (n = 12 each). One group was dialyzed with standard 1.36% Dianeal PD-2 (L-group); the other group was dialyzed with TB 1.36 (B-group). After break-in dialysis after catheter insertion, the animals were dialyzed twice daily with 30 mL of the designated dialysis solution for four weeks. White blood cell count with differentials and microbiological culture of the dialysate were examined once a week to detect peritonitis. A peritoneal equilibration test (PET) was performed on the eighth and thirty-sixth days. The dialysate was obtained at 0, 2, and 4 hours; a blood sample was taken at 0 hour. Peritoneal tissue specimens were obtained after the second PET. Histological score was calculated based on the degree of thickening of the peritoneum. Five rats in L-group and three rats in B-group suffered from peritonitis. Two other rats in B-group had complications and did not complete the experiment. Therefore, seven rats from each group finished the experiment, and the PET data was analyzed. The peritoneal transport property of B-group did not change over time, while, in L-group it became less permeable on the thirty-sixth day. Fibrotic thickening of the peritoneum was observed in both groups, however, the histological score was slightly lower in B-group. These results suggest that the bicarbonate dialysis solution may be less harmful to the peritoneum.

Animals↗
PubMed1996

The effect of peritonitis on the peritoneal membrane transport properties in patients on CAPD.

Peritonitis is known to acutely affect the transport characteristics of the peritoneal membrane, however, the long-term effects are not known. We studied the effect of peritoneal inflammation on mean dialysate-to-plasma creatinine concentration ratio (D/P), dialysate protein losses (DPL, g/week), and dialysate albumin losses (DAL, g/week), done at six weeks or more postepisode, in 152 patients [102 (67%) males, mean age 57 years (range 21-91)]. These patients were on continuous ambulatory peritoneal dialysis for a mean of twelve months (range 1-97). A total of 94 distinct peritonitis episodes were managed in 47 patients (31%). The number of patients with 0, 1, 2, 3, 4, and 5 episodes of peritonitis were 105, 29, 3, 6, 4, and 5. These episodes were treated with a standard protocol. There were no statistically significant differences between the D/P, DPL, or DAL between the groups. The parameters did not show any correlation to time on dialysis. Thus, in conclusion, peritonitis, if promptly treated, does not cause any permanent change in D/P, DAL, or DPL.

Adult↗
PubMed1996

Evaluation of healing and external tunnel histology of silver-coated peritoneal catheters in rats.

A previous study showed that silver-coating peritoneal catheters tended to decrease the incidence of early exit-site infections in rats. This study was designed to further evaluate the healing, biocompatability, and external tunnel morphology of standard and silver-coated catheters. Catheters were coated with silver by an ion beam-assisted process. Fourteen male Sprague-Dawley rats underwent implantation of either a standard or silver-coated double-cuff peritoneal catheter. Weekly observation and photographs documented exit-site characteristics. Erythema, exudate, loose fit, and poor hair growth were evidence of an inflamed exit. Overt infection was indicated by the presence of three or more of the following: erythema, purulent exudate, exuberant granulation tissue, loose fit, and poor hair growth. Animals were sacrificed at six weeks, and catheters were removed and processed for histology of the external tunnel. Multiple measurements were taken using a Filar eyepiece, and data were expressed as a mean of several readings. Inflammation, vascularity, and fibrosis were judged semiquantitatively. At the end of six weeks, six of the seven exits of the silver catheters showed excellent healing, while one exit site had signs of excessive inflammation. Four of the exit sites of the standard catheters healed well, two were inflamed, and one was overtly infected. The sinus tract of the standard and silver catheters had similar characteristics: keratinized and nonkeratinized epithelium lined the external part of the sinus tract and merged into granulation tissue. A fibrous sheath was noted in some sinus tracts between the granulation tissue and the cuff. The cuff evoked a foreign body reaction, with fibrosis, multiple giant cells, and vascularization. Poorly healing or infected sinus tracts had highly vascular granulation tissue with overlying exudate. The cuff of these catheters had marked inflammation and scanty giant cells, although collagen bundle thickness was similar to the well-healing catheters. In conclusion, silver-coating potentially enhances healing of the exit sites of peritoneal catheters. Additionally, the similarity of the tunnel histomorphology of standard and silver catheters confirms the favorable biocompatibility of silver.

Animals↗
PubMed1996

Protein catabolic rate in CAPD patients: comparison of different techniques.

Protein intakes of patients on continuous ambulatory peritoneal dialysis (CAPD) are estimated by protein catabolic rate (PCR) or dietary protein intake assessments (DPI). In this study we compared two approaches suggested by Randerson et al. for calculating PCR. One method incorporates urea generation rate (UG) and estimated dialysate protein losses (PCR), while the other includes UG and measured dialysate protein losses (PCR). We feel that calculating PCR2 is more convenient in practice. We studied 95 patients on CAPD, 49 men and 46 women, with a mean age of 56.5 years (range 26.6-84.5 years) and lean body mass of 41 kg (range 19-71 kg). Mean +/- SEM of PCR1, PCR2, and DPI were: 0.89 +/- 0.02, 0.86 +/- 0.02, and 0.90 +/- 0.04 g/kg standard weight (std wt)/day, respectively. PCR1 and PCR2 were highly and significantly correlated (r = 0.94, p = 0.0001). The difference of PCR1-PCR2 is plotted against dialysate protein loss, which reveals that PCR2 often underestimates PCR1 if dialysate protein loss is > 10 g/day, but this difference is minimum (< 0.1 g/kg standard weight/day) when the dialysate protein loss is < 15 g/day. We conclude that PCR2 is an easy and effective method to monitor nutritional status in the majority of CAPD patients as very few will have dialysate protein losses > 15 g/day.

Adult↗
PubMed1995

Expected white blood cell counts and differentials in a rat model of peritoneal dialysis.

OBJECTIVE: The purpose of this study was to establish baseline dialysate white blood cell (WBC) counts and differentials in noninfected rats on peritoneal dialysis (PD). DESIGN: Sixteen male Sprague-Dawley rats underwent PD in the first protocol, and eight from the 16 continued PD in the second through fourth protocols. At the beginning of the experiments, all animals had a PD catheter implanted and were initiated on PD with 1.5% dextrose dialysis solution twice daily. In the first protocol, WBC counts and differentials were assessed from day 4 to day 15 of dialysis in noninfected animals to establish "normal values" for such a rat model. In protocol 2, WBC counts and solute concentrations in small aliquots of dialysate obtained from the catheter were compared to values in well-mixed total drainage. Protocol 3 was designed to assess effects of dwell time on WBC counts. Protocol 4 examined the effect of glucose concentration of dialysis solution on WBC counts. RESULTS: In the first protocol, the mean dialysate WBC counts were significantly higher on the fourth day of dialysis, but stabilized below 2500 cells/mm3 by the eighth day. The percentage of neutrophils was stable around 20%-25%. In the second protocol, we found aliquots < 1 mL might underestimate the dialysate WBC count compared to the complete drainage. In the third protocol, WBC counts increased as cycle time became longer, but the percentage of neutrophils remained below 50%. In the fourth protocol, we did not find any effects of glucose concentration of instilled solutions on WBC counts and differentials. CONCLUSION: This study of WBC counts and differentials in noninfected rats on peritoneal dialysis establishes the range above which infection should be suspected. WBC counts increase with cycle time. Small dialysate aliquots may underestimate WBC counts. Glucose concentration does not effect WBC counts.

Animals↗
PubMed1995

Predicted and measured daily creatinine production in CAPD: identifying noncompliance.

OBJECTIVE: To evaluate the ratio of measured creatinine (Cr) production to predicted creatinine production as an index of noncompliance in patients on continuous ambulatory peritoneal dialysis (CAPD). DESIGN: A cross-sectional analysis. PATIENTS: One hundred and twenty-one patients on CAPD. MEASUREMENTS: We have calculated Cr production from measured Cr outputs in 24-hour collections of urine and dialysate. Predicted Cr productions were calculated from standard tables. Weekly KT/V urea and weekly Cr clearances were determined from the same 24-hour urine and dialysate collections. Lean body mass (LBM) was calculated from the Cr production. Serum albumin concentration was measured. RESULTS: The ratio of measured/predicted Cr production correlated positively and significantly with weekly KT/V urea, the protein equivalent of nitrogen appearance (PNA), weekly Cr clearance, and LBM. There was a decline in serum albumin concentration at ratios greater than 1.24, supporting the opinions of previous authors who have suggested that ratios greater than 1.24 are highly suggestive of noncompliance with the dialysis prescription. Defining noncompliance as a ratio greater than 1.24 implied that at least 5% of the female and 17% of the male patients were noncompliant. CONCLUSIONS: Declining serum albumin concentrations at higher ratios of measured/predicted Cr production support the opinion that this is an index of noncompliance. However, not all noncompliant patients necessarily have a ratio greater than 1.24. Weekly KT/V urea, weekly Ccr and LBM are all artifactually increased by "washout effects" if all exchanges are done only or mainly on the collection day.

Body Mass Index↗
PubMed1995

Modification of creatinine clearance by estimation of residual urinary creatinine and urea clearance in CAPD patients.

The use of creatinine clearance for adequacy of continuous ambulatory peritoneal dialysis (CAPD) requires consideration of the fact that a significant fraction of residual renal creatinine clearance is contributed by tubular secretion. We analyzed 123 peritoneal dialysis (PD) patients and corrected the residual renal creatinine clearance by averaging renal creatinine and urea clearance to estimate the glomerular filtration rate (GFR). Modified total creatinine clearance (peritoneal plus estimated renal GFR) was compared with total creatinine clearance (peritoneal plus total renal creatinine clearance). Modified and total creatinine clearances were not significantly different in patients with a total creatinine clearance less than 60 L/week/1.73 m2 body surface area (BSA), but a significantly lower modified total creatinine clearance was seen with patients having greater than 60 L/week/1.73 m2 BSA of total creatinine clearance. The correlation was better between KT/V and modified total creatinine clearance (r = 0.74) as compared to KT/V and total creatinine clearance (r = 0.67). We suggest that if creatinine clearance is used for peritoneal dialysis (PD) adequacy, the contribution of residual renal function should be calculated as the average of renal creatinine and urea clearance, thus estimating creatinine clearance only by the GFR. Further long-term studies are needed to confirm that modification of total creatinine clearance will better predict clinical outcome.

Creatinine↗
PubMed1995

Preliminary evaluation of silver-coated peritoneal catheters in rats.

Silver is known to have powerful antibacterial properties against a variety of micro-organisms and has a low toxicity and a favorable biocompatibility profile. This study was designed to evaluate the effectiveness of silver-coated catheters in preventing early exit-site infection and to assess tunnel morphology. Seven male Sprague-Dawley rats underwent simultaneous implantation of two double-cuffed, silver-coated silicone rubber and standard silicone rubber catheters. Weekly observations and photographs documented exit-site characteristics. The animals were sacrificed and catheters removed and processed for histopathology of the external tunnel at 5 weeks. Exit sites of silver-coated catheters tended to have less inflammation and infection and healed better than those of uncoated catheters; however, these data did not achieve significance using the Wilcoxon signed-rank test. Sections of the external tunnel of well-healing exit sites showed an epithelialized tract with granulation tissue near the cuff and significant invasion of the external cuff by collagen with a mild neutrophilic inflammatory response. In contrast, the histology of the external tunnel of infected exists revealed exudate overlying inflammatory granulation tissue and a variable degree of fibrosis of the cuff. When the exit sites appeared similar, no significant histopathological differences in sinus tract and cuff morphology were noted with either silver or standard catheters. In conclusion, these findings suggest that silver coating of catheters may decrease the incidence of early exit-site infections and allow better ingrowth of the catheter.

Animals↗
PubMed1995

Rat model of peritoneal fibrosis: preliminary observations.

The aim of this study was to establish a rat model of peritoneal fibrosis. After insertion of peritoneal catheters into 18 rats, the rats were divided into three groups. All animals were dialyzed twice a day with 4.25% Dianeal containing heparin. Group 1 rats (control) received antibiotics (vancomycin and gentamicin) in each exchange: group 2 rats were inoculated with Escherichia coli (5 x 10(6) in 5 mL of saline) at the beginning of the study; group 3 rats were treated with antibiotics after Escherichia coli inoculation; they also received a second inoculation of Escherichia coli after the second week of the study. By the end of the second week, group 2 rats were sacrificed because of catheter problems. Group 1 and 3 rats were sacrificed after 4 weeks of dialysis. A weekly peritoneal equilibration test (PET) was performed in each rat. The comparison of the PET results from the beginning and end of the study showed an increased permeability to glucose (p < 0.05) and total protein (p < 0.05) in group 3, which was not noted in group 1. In histology samples there was only delicate fibrosis with cellular infiltration in the peritoneum in group 1 rats. These changes were much more prominent in group 3 rats. This study suggests that E. coli peritonitis causes peritoneal fibrosis in rats, but to have a sclerosing encapsulating peritonitis (SEP) model this experiment must be carried out for a longer time.

Animals↗