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Biomedical subjects

H Kupper

Publications and source records attributed to H Kupper.

At least 37 records · Page 2Linked to original sources
PubMed1986

Double staining technique using a combination of indirect and direct immunofluorescence with monoclonal antibodies.

A simple method for double staining by immunofluorescence is described. If for double staining using monoclonal antibodies of the same species only one antibody is conjugated with FITC or TRITC, a combination of indirect and direct immunofluorescence is possible. For cell staining the following incubation steps are carried out: Monoclonal antibody I (unlabelled, mouse), anti-mouse immunoglobulin serum FITC- or TRITC-conjugated, normal mouse serum for blocking of free binding sites of the anti-mouse immunoglobulin, and monoclonal antibody II (mouse) which is conjugated with an alternative fluorochrome. The use of this method is demonstrated for investigation of single cell suspensions (performed as a slide test) and of cryostat sections.

Animals↗
PubMed1985

[Characterization of monoclonal antibodies to human monocytes].

A set of monoclonal antibodies reacting with human peripheral blood monocytes is characterized. Two of these antibodies also bind to a fraction of granulocytes (BL-M/G-1) respectively to all of them (BL-M/G-2). On the other hand the BL-M-3 appears to be specific for monocytes only. All three monoclonals do not react with enriched B and T lymphocytes, platelets, erythrocytes and several lymphoid or erythroid permanent cell lines. The antigens recognized by all three monoclonals are expressed by monoblastic cell line U-937, whereas the myeloid line HL-60 and the cell line KG-1 express only antigen recognized by monoclonal antibody BL-M/G-2.

Animals↗
PubMed1985

[Characterization of monoclonal antibodies to human Ia antigens].

A set of monoclonal antibodies reacting with human Ia antigens is characterized. All of these antibodies (BL-Ia/1-3) have the same binding pattern to different types of leukocytes and a set of lymphoid and myeloid cell lines. Monoclonal antibody BL-Ia/1 can be used for complement dependent cytolysis of B cells and Ia+-T cells.

Animals↗
PubMed1984

[Characterization of a series of monoclonal antibodies against human T cells].

A set of 6 monoclonal antibodies is characterized reacting only with human T-cells but not with B lymphocytes. The antibody BL-T1 binds to the E receptor, being there on all mature T cells and on the majority of thymocytes. All respectively the majority of peripheral T cells but only subpopulations of thymocytes are recognized by the monoclonals BL-T2, BL-T3, BL-T4 and BL-T5. For identification and quantification of mature human T cells especially the antibody BL-T2 is suitable. The antigen recognized by BL-E1 is missing on peripheral B lymphocytes but expressed not only on T cells but also on erythrocytes, monocytes and a fraction of granulocytes. The monoclonals discussed are compared with the monoclonals for identification of leucocyte differentiation antigens (cluster of differentiation) characterized on the 1st Int. Workshop on Human Leucocyte Differentiation Antigens (Paris, 1982).

Animals↗
PubMed1984

[Nonparametric estimation of 1-dimensional continuous distribution density functions using the continuous LOLINREG approximation].

A nonparametric method for estimation of one-dimensional continuous probability distribution functions is presented. Procedures for calculation of estimation of the unknown distribution function and the distribution density will be discussed in their application. 2 items are what type of weight function may be chosen for the proposed local-linear continuous approximation of the empirical distribution function by the least squares method (LOLINREG), and upon what value of bandwidth- or smoothing parameter one optimally should settle. The latter problem is practically very important with respect to the quality of the estimation results. Examples of simulated measurements which come from a standardized normal distribution as random numbers serve to demonstrate the mode of working, the advantages as well as the limits of the presented continuous LOLINREG-approximation.

Hip Dislocation, Congenital↗
PubMed1983

[Slide test for immunofluorescent microscopy and immunoenzyme detection of cell-membrane antigens].

Poly-dimethyl-diallyl-ammoniumchloride - coated glass slides cause a strong adherence of living cells in consequence of electrostatic interactions. Immunological reactions of adherent cells - we used immunofluorescence and immunoperoxidase techniques to demonstrate membrane antigens - are not impaired. The slide test is suitable for routine quantitation of B and T cells in the peripheral blood and for the selection of hybridoma antibodies against cellular antigens. The slide test is time saving and needs minimal volumes of cells and antisera.

Antigens, Surface↗
PubMed1983

[Immunohistological detection of T lymphocytes with monoclonal antibody BL-T2].

Using the monoclonal Pan-T cell antibody BL-T2 T lymphocytes are identified on tissue sections with an indirect immunofluorescence technique. The T cell areas between the lymphoid follicles (B cells) are stained on cryostat sections of human palatine tonsils. A conventional heterologous anti-Ig antiserum was used for the detection of B lymphocytes. The identification of T and B lymphocytes by immunofluorescence allows statements about the distribution of both populations in tissue sections.

Antibodies, Monoclonal↗