Search PubMed⌕ Search

Biomedical subjects

H Kumada

Publications and source records attributed to H Kumada.

At least 145 records · Page 8Linked to original sources
PubMed1991

Effect of repeated transcatheter arterial embolization on the survival time in patients with hepatocellular carcinoma. An analysis by the Cox proportional hazard model.

One hundred fifty-eight patients with hepatocellular carcinoma (HCC) were treated by transcatheter arterial embolization (TAE) as repeatedly as possible. Survival rates at the end of the first, second, and third year were 76.5%, 54.5%, and 41.1%, respectively. In 142 patients with repeated TAE, a significantly increased number of patients with complete necrosis of tumor was observed after repetition of the therapy. Adjusting the imbalance in prognostic factors among patients by using Cox proportional hazard model, it proved that the best response during the repeated therapy, rather than the first response, was significantly associated with survival period of the patients. Aside from the factor of response to the treatment, tumor size was the worst prognostic factor at the time when diagnosis was made. Other significant factors were portal vein invasion by HCC and bilirubin. The survival period of patients with HCC treated by repeated TAE was, therefore, affected by cancer factors, liver cirrhosis factors, and therapy-responsiveness factors. It is concluded that even if complete necrosis of tumor is not obtained after the first trial, repetition of TAE is an effective measure for prolonging of survival time in patients with HCC.

Adult↗
PubMed1991

Effect of interferon administration on serum hepatitis C virus RNA in patients with chronic hepatitis C.

Hepatitis C virus RNA as detected by reverse transcription and nested polymerase chain reaction was monitored in 16 patients with chronic hepatitis C treated with interferon. Hepatitis C virus RNA became undetectable after 4 to 8 wk of interferon administration in 13 of the 16 patients. During 6 mo of follow-up, 5 of the 13 patients who became negative for hepatitis C virus RNA after interferon administration remained negative, and all five continued to have normal ALT levels. Repeat liver biopsy in these five patients revealed histological improvement. Antibody to hepatitis C virus, which was initially positive in all treated patients, fell to undetectable levels in three of the five patients. In contrast, aminotransferase levels rose again in all eight patients who had become hepatitis C virus RNA negative but had again exhibited hepatitis C virus RNA after completion of therapy. In 16 untreated patients, hepatitis C virus RNA remained detectable. These results indicate that detection of hepatitis C virus RNA may be useful as a marker of viral replication in chronic hepatitis C; they also suggest that interferon should again be administered to patients who become hepatitis C virus RNA negative on treatment but again exhibit this marker of viral replication when treatment is stopped.

Adult↗
PubMed1991

[Clinical features and viral markers in patients with chronic hepatitis positive for both HBs antigen and anti-C-100 antibody].

Among 612 patients with chronic hepatitis type B, 24 patients tested positive for anti-C-100 antibody. Clinical features and viral markers of these 24 patients were studied. Anti-C-100 antibody was detected more often in patients negative for HBe antigen than those positive for this antigen (22/385 vs. 2/227 [P less than 0.01]). HCV RNA, as detected by reverse transcription and nested polymerase chain reaction, was positive in 20 of 22 patients negative for HBe antigen whereas it was detected in only one of two patients positive for HBe antigen. In a patient who seroconverted from HBe antigen to anti-HBe HCV RNA was undetectable in the HBe antigen positive phase and had become detectable after seroconversion. These data indicate that both hepatitis virus (type B and type C) can co-infect in a patient and that type C becomes active after the seroconversion from HBe antigen to anti-HBe.

Adult↗
PubMed1990

Hepatoblastoma in an 82-year-old man. An autopsy case report.

An autopsy case of adult hepatoblastoma is presented. The patient was an 82-year-old male with chronic hepatitis of 7 years' duration. The liver tumor was detected 6 months before death. Autopsy revealed a large hepatic tumor occupying about 80% of the entire liver. Histologically, the tumor showed typical features of mixed epithelial- and mesenchymal-type hepatoblastoma. The epithelial component consisted of fetal and embryonal cell types. The mesenchymal component showed primitive spindle-shaped cells with various degrees of cellularity. Chondroid areas and a few foci of osteoid formation were also present.

Aged↗
PubMed1989

Purification and characterization of extracellular glucosyltransferase synthesizing water-insoluble glucan from Streptococcus rattus.

An extracellular glucosyltransferase synthesizing water-insoluble glucan (GTF-I) was purified from the culture supernatant of Streptococcus rattus strain BHT (mutans serotype b) by hydroxylapatite chromatography, DEAE-Toyopearl chromatography and preparative isoelectric focusing. The Mr of GTF-I was 155,000 by SDS-PAGE and the isoelectric point was pH 4.9. The specific activity, the optimum pH and the Km value for sucrose were 10.0 i.u. (mg protein)-1, 6.5 and 2.4 mM, respectively. The enzyme synthesized a water-insoluble glucan consisting of 69.4 mol% 1,3-alpha-linked glucose, 23.6 mol% 1,6-alpha-linked glucose, 2.6 mol% 1,3,6-alpha-branched glucose and 4.4 mol% non-reducing terminal glucose, and also a small amount (3% of the total glucan) of soluble glucan with 82.4 mol% 1,6-alpha-linked glucose. The Mr and pI values of purified GTF-I were identical with those of the enzyme in the culture supernatant.

Chromatography, Gel↗
PubMed1989

Chemical and biological properties of lipopolysaccharide, lipid A and degraded polysaccharide from Wolinella recta ATCC 33238.

Lipopolysaccharide (LPS) was isolated and purified from Wolinella recta ATCC 33238 by the phenol-water procedure and RNAase treatment. The sugar components of the LPS were rhamnose, mannose, glucose, heptose, 2-keto-3-deoxyoctonate (KDO) (3-deoxy-D-manno-octulosonate) and glucosamine. The degraded polysaccharide prepared from LPS by mild acid hydrolysis was fractionated by Sephadex G-50 gel chromatography into three fractions: (1) a high-molecular-mass fraction, eluting just behind the void volume, consisting of a long chain of rhamnose (22 mols per 3 mols of heptose residue) with attached core oligosaccharide; (2) a core oligosaccharide containing heptose, glucose and KDO, substituted with a short side chain of rhamnose; (3) a low-molecular-mass fraction containing KDO and phosphate. The main fatty acids of the lipid A were C12:0, C14:0, 3-OH-C14:0 and 3-OH-C16:0. The biological activities of the LPS were similar to those of Salmonella typhimurium LPS in activation of the clotting enzyme of Limulus amoebocytes, the Schwartzman reaction and mitogenicity for murine lymphocytes, although all the biological activities of lipid A were lower than those of intact LPS.

Bacteroidaceae↗
PubMed1989

[Serological classification of Bacteroides gingivalis and purification of group specific antigens].

Bacteroides gingivalis were serologically investigated by the immunodiffusion technique using autoclave-extracted antigens and antisera against whole cells. Fifteen strains of B. gingivalis used in this study, were classified in two distinct serological groups. Eleven strains include strain 381, ATCC 33277 were classified Group A and four strains include strain Su63 were classified in Group B. The group specific antigens from B. gingivalis strain 381 (Group A) and Su63 (Group B) were partially purified from autoclave extracts by 75% ethanol precipitation and anion exchange chromatography, and were identified as polysaccharides composed of glucose (82.3%, 23.7%), rhamnose (7.1%, 12.7%), galactose (4.9%, 18.2%), mannose (2.3%, 1.7%), glucosamine (0.4%, 28.0%) and galactosamine (3.0%, 15.3%).

Antigens, Bacterial↗
PubMed1989

[Remission state and five-year survival after transcatheter arterial embolization therapy in patients with hepatocellular carcinoma].

Repeated transcatheter arterial embolization (TAE) therapy was done for 138 cases with hepatocellular carcinoma. Relationship was investigated between long term survival more than 3 years and tumor necrosis rate estimated on radiological images (superselective angiogram and/or computerized tomography) after therapy. Excellent tumor necrosis (radiological disappearance of viable tumor) was attained in 35 out of 138 cases through repeated TAE. When "remission" is defined as excellent tumor necrosis and normal alphafetoprotein value lasting for more than one year, remission state was attained in 17 cases (12.3%). Cumulative three-year survival rate was 37.0%, and 5-year survival was 26.5%. Out of 37 three-year-survivors, only 12 cases (32.4%) experienced remission state in their clinical course. On the other hand, 7 cases (77.7%) of 9 five-year-survivors experienced remission at least one time. Though good tumor necrosis and 3-year survival were achieved by only performing repeated TAE therapy, "remission" state was important and necessary to get 5-year survival.

Aged↗
PubMed1988

Hepatic squamous cell carcinoma with hypercalcemia in liver cirrhosis.

Primary squamous cell carcinoma of the liver is exceedingly rare and has previously been reported in association with hepatic teratoma, hepatic cyst or hepatolithiasis. This paper describes an autopsy case of squamous cell carcinoma which developed with hypercalcemia in a cirrhotic liver. This cancer was characterized histologically, immunohistologically and ultrastructurally, and was found to exhibit immunofluorescence positivity for anti-epidermal keratin monoclonal antibody, together with the presence of tonofilaments scattered sparsely in the cytoplasm of the cancer cells.

Antibodies, Monoclonal↗
PubMed1987

Purification and characterization of extracellular glucosyltransferase from Streptococcus mutans serotype b (subspecies rattus).

An extracellular glucosyltransferase (GT-S) synthesizing water-soluble glucan was purified from the culture supernatant of Streptococcus mutans BHT (serotype b, subsp. rattus) by DEAE-Sepharose chromatography and preparative isoelectric focusing. The Mr of the enzyme was 155,000 and the pI was 4.5. The GT-S had a specific activity of 10.2 i.u. (mg protein)-1, an optimum pH of 6.0 and a Km value of 0.8 mM for sucrose, and was activated twofold by dextran T10. The GT-S was immunologically partially identical with the corresponding enzymes in crude preparations from serotypes c, e and f. The glucan synthesized de novo from sucrose by the GT-S was water-soluble and consisted of 29 mol% of non-reducing terminal, 49 mol% of 1,6-alpha-linked, 11 mol% of 1,3-alpha-linked and 11 mol% of 1,3,6-alpha-branched glucose residues.

Animals↗