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Biomedical subjects

H Kubo

Publications and source records attributed to H Kubo.

At least 361 records · Page 20Linked to original sources
PubMed1985

Elimination of silica gel from gangliosides by using a reversed-phase column after preparative thin-layer chromatography.

A simple and effective procedure has been developed for eliminating silica gel from gangliosides after preparative thin-layer chromatography. Gangliosides were extracted from the scraped silica gel with chloroform-methanol-water 10:10:3 (by volume) and dried. The residue was suspended in 0.1 M NaCl and centrifuged. After filtration through a sintered glass funnel, the supernatant was applied to a reversed-phase column. The column was washed with 0.1 M NaCl, water, and methanol-water 1:1 (v/v). Gangliosides were eluted from the cartridge with methanol with yields of 92% and 90% in terms of dry weight and sialic acid content, respectively. Almost all of the silica gel contamination was eliminated from gangliosides by this procedure.

Animals↗
PubMed1985

[Late diastolic mitral regurgitation: pulsed Doppler echocardiographic study].

Since the description of late diastolic mitral regurgitation (MR) detected by left ventriculography, the risk of overestimating systolic MR has been pointed out. However, it has not been well studied by pulsed Doppler echocardiography, though this method is thought to be extremely useful to analyze systolic MR. The purpose of this study was to evaluate the usefulness of pulsed Doppler echocardiography in assessing late diastolic MR and to clarify the pathophysiology of this MR. With the probe in the apical position, the blood flow pattern at the left atrial outflow tract was investigated in 226 consecutive cases who underwent left ventriculography. Late diastolic MR, which could not be attributed to ventricular premature contraction, was observed in 10 cases (4.4%) by left ventriculography. Meanwhile, by pulsed Doppler echocardiography, late diastolic disturbed flow directing towards the left atrial cavity in late diastole was detected in 15 cases (6.6%) including 10 cases with positive left ventriculographic MR. Among these 15 cases, 14 (93%) had atrial fibrillation and exhibited this MR only in the cardiac cycle with prolonged R-R intervals. The limitation in number of cardiac cycles which could be analyzed and the rapid heart rate eliminating the appearance of the beat with prolonged R-R intervals may be the reasons for the paucity of late diastolic MR by left ventriculography. Ten cases (66%) of late diastolic MR, including one with sinus rhythm, had aortic regurgitation, three cases showed high-grade systolic MR, and two had atrial septal defect. The simultaneous recording of pulmonary arterial wedge pressure and left ventricular pressure in three patients show a reversal of pressure gradient across the mitral valve in late diastole when the R-R interval was prolonged. In conclusion, pulsed Doppler echocardiography is useful in detecting late diastolic MR, and in reducing overestimates of systolic MR by ventriculography which was induced by erroneous addition of late diastolic MR, because Doppler method has better time resolution in phase analysis. We believe that incomplete premature mitral valve closure caused by a delay in left ventricular contraction following prolonged R-R intervals, and the reversal of left atrial-left ventricular pressure gradient induced by left ventricular over-filling played important roles in the occurrence of late diastolic MR.

Adult↗
PubMed1985

[Clinical study of recombinant interferon alpha-2 (Sch 30500) in advanced gynecological cancers].

Recombinant interferon alpha-2 (Sch 30500) was administered to 29 patients with advanced gynecological cancers (14 patients with cancer of the cervix, 8 with ovarian cancer, 4 with uterine sarcoma, 2 with endometrial cancer and 1 with unclassified cancer). No antitumor effects (CR and PR) were noted in 23 evaluable patients. Side effects observed were fever, tachycardia, diarrhea, chills, general fatigue, anorexia, nausea and vomiting. In some patients, leukopenia, decrease of hemoglobin and elevation of SGOT and SGPT were observed. No production of antibody for Sch 30500 was noted.

Adult↗
PubMed1985

[Pelvic lymph node metastasis of cervical adenocarcinoma and adenosquamous carcinoma].

Thirty-one cervical cancers, comprised of 16 adenocarcinomas and 15 adenosquamous carcinomas, were clinicopathologically examined to demonstrate the characteristic features of pelvic lymph node metastasis. The average number of metastatic nodes in each case was 2.6. The most common loci of metastases were the nodi lymphatici obturatorii, iliaci externi and ligamenti cardinales. The histologic distributions in the metastatic nodes suggested that the adenocarcinoma element was more likely to metastasize to the nodes than its squamous counterpart.

Adenocarcinoma↗
PubMed1985

[Methodological study for human in-vitro fertilization related to maturity of gametes].

The correlations with maturity of gametes and fertilizability was studied. Specimens of semen were treated by several methods for induction of capacitation and acrosome reaction (AR). Follicular oocytes were recovered with endoscopic aspiration procedures, and divided to two groups: 1, maturing in vivo (preovulatory) and 2, immature. Immature oocytes were divided further into two subgroups: 1, germinal vesicle breakdown (GVBD) and 2, first polar body formation (FPB), after maturation culture. The sperms treated with the centrifuge-trypsinization-layering method showed a higher incidence of in-vitro fertilization (69.2%) and cleavage (51.3%) of human preovulatory eggs compared with other capacitation inducing methods. It was found that human spermatozoa that have undergone the acrosome reaction prior to zona attachment are capable of fertilizing eggs under the in vitro conditions. On the estimation of fertilizability with reference to egg maturity, 52.5% (21/40) of IMIV-GVBD and 64.7% (22/34) of IMIV-FPB eggs were fertilized. However, only 12.5% (5/40) of IMIV-GVBD and 11.8% (4/34) of IMIV-FPB eggs cleaved. These results showed that IMIV eggs had fair fertilizability, but no developmental capacity after the first cleavage. This may due to prematurity of the cytoplasm in IMIV eggs.

Animals↗
PubMed1984

Tissue distribution of hydrazine and its metabolites in rats.

The tissue distribution and the urinary excretion of hydrazines, hydrazine, acetylhydrazine and 1,2-diacetylhydrazine, were determined by mass fragmentography using a gas chromatography-mass spectrometer equipped with a multiple ion detector-peak matcher. Using the compounds labeled with a stable isotope as an internal standard, namely the isotope dilution method, made it possible to estimate trace amounts of hydrazine and its metabolites in the tissues. Significantly high levels of all hydrazines were detected in the kidney. Especially, acetylhydrazine, a metabolite of hydrazine, accumulated to a great extent in the kidney. Free hydrazine which was liberated from acetylhydrazine was detected both in the tissues and in the urine after the administration of acetylhydrazine. This demonstrates clearly that the metabolic pathway between hydrazine and acetylhydrazine is reversible.

Acetylation↗
PubMed1984

Calcium pyrophosphate dihydrate deposits in the cervical ligamenta flava causing myeloradiculopathy.

Three cases of cervical myeloradiculopathy associated with multiple calcified nodules containing identified calcium pyrophosphate dihydrate (CPPD) crystals in the ligamenta flava are described, with a comprehensive review of the 12 cases of this entity reported to date. The disease is characterized by: 1) oval or triangular areas of radiodensity in the posterior aspect of the cervical canal as seen in the lateral x-ray films and laminograms; 2) hemispherical areas of high density located almost symmetrically in the paramedial portion of the posterior spinal canal on computerized tomography scans; and 3) CPPD crystals in the nodules. It occurs independently or in association with cervical spondylosis or ossification of the posterior longitudinal ligament.

Aged↗
PubMed1984

[Indication of aorto-coronary bypass surgery for the infarcted myocardium: assessment using exercise T1-201 myocardial imagings].

Using exercise thallium-201 myocardial imagings, 23 patients with previous myocardial infarction were evaluated before and after aorto-coronary bypass graft (ACBG) surgery to assess the indication of the operation for the infarcted myocardium. The patients were categorized as group I with regional hypoperfusion and redistribution in the infarcted site (11 patients), and group II with regional hypoperfusion without redistribution (12 patients). Changes in the exercise tolerance before and after ACBG: By the criteria of Canadian Cardiovascular Society, clinical symptoms improved in nine of 11 patients (82%) in group I, and eight of 12 patients (67%) in group II. Pressure rate product improved significantly in group I (201 +/- 28% to 219 +/- 21% p less than 0.05), whereas it showed no significant change in group II (200 +/- 44% to 203 +/- 35%). Changes in the global left ventricular ejection fraction before and after ACBG: There was a significant increase in group I (63 +/- 11% to 68 +/- 9% p less than 0.02), whereas no significant change in group II (53 +/- 16% to 54 +/- 14%). Changes in myocardial perfusion in the infarcted segment before and after ACBG: Group I showed an increase in myocardial perfusion in all of the 11 (82%) except two patients with the occluded graft, and group II showed an increase in only two of the 12 patients (17%). Changes in wall motion abnormality in the infarcted segment before and after ACBG: Improvement of the wall motion abnormality was observed in eight of the 11 patients (73%) in group I, whereas only one of the 12 patients (8%) in group II. It is suggested that preoperative redistribution of the infarcted site in the delayed image suggests myocardial viability, and allows us to expect the postoperative improvement of myocardial hypoperfusion and wall motion abnormality.

Adult↗
PubMed1984

Acute effects of mercuric compounds on preimplantation mouse embryos in vitro.

The effects of mercuric compounds on the proliferation and protein synthesis of mouse blastocysts were examined in vitro by treating the embryos with methylmercury (MMC) or mercuric chloride (MC). Late blastocysts were exposed to various concentrations of mercuric compounds for 24 hr. and incubated for another 24 hr. in a mercury-free medium. The protein synthesis of the mercury-treated blastocysts was measured by counting the incorporation of L-[35S]methionine into the acid-insoluble protein fraction of a cell. 0.1 microM of MMC was equivalent to 20 microM of MC with regard to the inhibitory effect on proliferation, and equivalent to 2 to 5 microM of MC after 24 hours' culture, and 10 to 20 microM after 48 hours' culture with regard to the inhibitory effect on protein synthesis of the blastocysts. The results showed that MMC was approximately 200 times more toxic than MC with regard to cell number proliferation, and 20 to 50 times so with regard to protein synthesis capability.

Animals↗
PubMed1984

[Deoxyribonucleic acid and protein synthesis in delayed mouse blastocysts in vitro].

Developmental ability, DNA and protein synthesis were examined in mouse blastocysts which were delayed by proteinase inhibitors (nitrophenol-p-guanidino benzoate (NPGB) & soybean trypsin inhibitor. Cell division is depressed in the culture which contains proteinase inhibitors: Delayed blastocysts showed no increase in number after 5 days of culture. Mitotic figures were absent 120 hours after culture. On the other hand, most of the blastocysts showed a substantial increase in number in the first few days following the onset of delay. The DNA synthesis of nuclei in delayed blastocysts was measured by autoradiography incorporating [3H]-TdR. Although the incorporation rate was 54 +/- 8 cells/embryo (83 +/- 7%) on the first day of culture, it was decreased to 1/3 (36 +/- 5, 24 +/- 1%) on the 10th day of culture. Protein synthesis was measured with the incorporation rate of [35S]-methionine. The incorporation rate of delayed blastocysts declined sharply on the 5th day of culture (1.6 +/- 0.4 pmol/embryo/hr.) in contrast to that of normal implanted blastocysts (2.5 +/- 0.3 pmol/embryo/hr.). Qualitative changes in synthesized protein were estimated by SDS-PAGE. About 110 of the autoradiographic bands on 1-D gel were observed in the 4th day blastocysts, and actin is the major protein synthesized, located between 50,000 and 55,000 daltons. No differences in these bands were seen between normal and delayed blastocysts. However, two-dimensional gel showed that the qualitative patterns of protein synthesis of delayed embryos are significantly different from those of normal implanted blastocysts. In delayed blastocysts, trophoblast-specific spots varied versatile. Above all, protein synthesis of trophoblast in delay is selectively suppressed by proteinase inhibitors, but not ICM. Therefore, delayed blastocysts are not always metabolically dormant.

Animals↗
PubMed1984

[Standardized radiation treatment method for carcinoma of the uterine cervix].

Due to a world-wide information exchange on cancer therapy, a standardized treatment method for cancer is desirable. Radiation therapy for carcinoma of the uterine cervix is usually in combination with external and intracavitary irradiation. A basic treatment regiment for carcinoma of the uterine cervix with external and intracavitary irradiation was established by the major Japanese institutions. The consensus include: size and shape of irradiation field, dose, the reference from which to calculate the point A dose the external orifice of cervix is point A dose is regarded as the minor dose between right and left point As', categorized treatment methods with RALS according to cancer stage, the post operative irradiation method. This standardized treatment method will be applied for data analysis at each institution and will promote the further development of radiation therapy.

Brachytherapy↗