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Biomedical subjects

H Kramer

Publications and source records attributed to H Kramer.

At least 37 records · Page 2Linked to original sources
PubMed1982

[Activation and cell-aggregation of mononucleus cells after short-term stimulation with mitogen and tuberculin].

The aim of the study was the description of morphological changes in mononukleated cells after short-term stimulation by mitogen and tuberculin visible by light- and electronmicroscopy and in differences between nucleus areas measured by planimetry. Already 1 hr. after p.h.a. stimulation lymphocytes forme clusters in which the mononucleated cells were present mostly as irritated and exhausted cells. In PPD-stimulated lymphocytes clusters were formed after 16 to 24 hrs. These cells were activated. Within clusters strong intercellular contacts were visible using the electron-microscopy. The results of planimetry in regard to nucleus areas of lymphocytes and monocytes were significantly dependent from the kind of mitogen or antigen and the duration of incubation time (analysis of variance).

Cell Aggregation↗
PubMed1982

[Analysis of relationships between physiologic time series in various force loads].

The described analysis of physiological time series gives an example of how the relations between such series can be computed. We had performed force-varied isometric hand-grip contractions. After testing the corresponding prerequisites we investigated the interrelations between the force-induced responses of heart rate, blood pressure, pulse-transit time and force.

Blood Pressure↗
PubMed1981

[Results of studies on dogs after unilateral allogeneic lung transplantation without immunosuppression].

The observations and the results after allogenic lung transplantation in 24 receivers were reported. The animals were not treated immuno-suppressively. They survived 6.2 days on an average and died in consequence of the rejection. The transplant is progressively threatened of bacterial infections after the beginning of the rejection even in case of an operation under sterile conditions. The investigations confirmed a narrow correlation between the leucocyte count and the course of the rejection. However, the condition of the transplant can be estimated approximately certainly by means of complex clinical methods of examination.

Animals↗
PubMed1979

[Immunologic diagnosis].

The development of the immunologic basic research and a deeper insight into the pathogenesis of diseases with changed immune reactivity led to an intensivation of the clinic-immunologic in-vivo- and in-vitro-diagnostics. At the instance of the pulmonary diseases according to the classification of Gell and Coombs the immunologic-diagnostic possibilities and problems are shown. Here is referred to the importance of the use of standardized tests and of the wide replaceability of in-vivo-techniques by in-vitro-methods.

Anti-Glomerular Basement Membrane Disease↗
PubMed1979

[Evaluation of lymphocyte transformation test: relationship between nucleus and cell sizes of PHA-stimulated lymphocyes (author's transl)].

A methodological comparision of evaluation of lymphocyte transformation test is described. Three experts examined smear-slides of cells from five lymphocyte cultures and the corresponding controls in a blind study. In the morphological differentiation of 100 cells per slide in comparison to 1 000 cells per slide it was found a good intraindividually but a unsatisfactory interindividually correlation. 1 000 cells from stimulated and non-stimulated cultures shows the greatest difference for the parameter "total cell size" and the lowest difference for the "nucleus plasma relation" by the planimetrically imeasurement.

Cell Nucleus↗
PubMed1979

[Investigations for the use of micromodification of lymphocyte transformation test in patients with lung disease (author's transl)].

In a preliminary study a micromodification of lymphocyte transformation test was examined for use in patients with specific and nonspecific lung diseases. Investigation was carried out about the influence of different conditions in the cell culture system. A micromodification was defined and introduced in the clinic simultaneously to the conventional macromodification of lymphocyte transformation test. This micro lymphocyte transformation test is of use for determination of cell mediated immunity. However, in the described form it is doubtful for the detection of present specific sensibilization.

Dose-Response Relationship, Drug↗
PubMed1978

[Use of delayed cutaneous hypersensitivity skin test and in vitro assays of lymphocyte function to tumour associated antigen in patients with lung cancer (author's transl)].

Twenty one patients with lung cancer and twelve patients with unspecified lung disease were evaluated by means of delayed cutaneous hypersensitivity skin tests with tumour associated antigen. Nine of these patients with lung cancer and seven healthy persons were evaluated by in vitro assays of lymphocyte function to tumour associated antigen (TAA) from "Cancerotest Dessau". We did not find a statistically significant lymphocyte transformation to the testing concentration of TAA. For the same patients with lung cancer and controls we found statistically significant results of the mean lymphocyte transformation to phytohemagglutinin. It has been shown that the delayed cutaneous hypersensitivity skin tests demonstrated "false positive" reactions to TAA. It is possible that this is an expression of the irritative effects of TAA. It is estimated that the application of the antigens in this form presented and in this chosen examination conditions, does not allow for definite diagnostic assessment to be expected.

Antigens, Neoplasm↗