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Biomedical subjects

H Koprowski

Publications and source records attributed to H Koprowski.

At least 505 records · Page 28Linked to original sources
PubMed1972

Effect of environmental pH on the efficiency of cellular hybridization.

The hybridization of a human and mouse cell was strikingly pH-dependent, with a well defined optimum at (about) pH 7.6-8.0. The yield of hybrid cell colonies (1 per 500-2000 heterokaryocytes) was several hundred times greater than that obtained at pH 6.8-7.2. Although there was a significant effect on the efficiency of cell fusion, the critical time for the pH effect was in the first 4-8 days after fusion, presumably while viable hybrids were being formed from the multinucleated heterokaryocytes.

Animals↗
PubMed1972

Antibody response in vitro to an animal virus: production of rabies virus neutralizing antibodies by mouse cells in culture.

Rabies virus neutralizing antibodies were produced in vitro by the exposure of mouse spleen cells to live and inactivated rabies virus suspensions and to sheep erythrocytes coated with rabies virus. These antibodies did not neutralize two other rhabdoviruses: Kern Canyon and vesicular stomatitis viruses, and were precipitable by treatment with an antiserum to mouse IgG. Removal of "glass-adhering" cells from mouse spleen cell suspensions abolished the antibody response, which could be restored by the addition of mouse peritoneal exudate cells, rich in macrophages.

Animals↗