Biomedical subjects

H Kogo

Publications and source records attributed to H Kogo.

107 records · Page 6Linked to original sources

PubMed1980

[Inhibitory action of non-steroidal anti-flammatory drugs on prostaglandin synthesis and release (author's transl)].

Inhibitory action of non-steroidal anti-inflammatory drugs (NSAID)on the synthesis and release of prostaglandin (PG) was studied both in vivo and in vitro. In the experiment with PG synthetase from beef seminal vesicle, the potency of NSAID on the inhibitory action of PGE synthesis was: flurbiprofen greater than 4-(p-chlorophenyl)-2-phenyl-5-thiazoleacetic acid (CH-800) greater than indomethacin greater than diclofenac-NA greater than aspirin. Inflamed synovial tissue, as induced 6 hours after carrageenin injection into the knee joint cavity of rats was used to examine the inhibitory action of NSAID on PGE release from the tissue. Both in vivo and in vitro, CH-800 was the most potent and flurbiprofen and indomethacin were next potent of the five drugs. Effects of NSAID on the excretion of PGE and PG main urinary metabolite (PGMUM) in rat urine were determined. The excretion of urinary PGE was significantly decreased by administration of NSAID. Flurbiprofen and CH-800 had the most potent inhibitory action on the excretion of PGEMUM, but the urinary excretion of PGFMUM was not significantly inhibited by NSAID. Thus, the inhibitory effects of NSAID associated with PG synthesis were generally in proportion to the effect of the drugs with PG synthetase, however, there were differences regarding PG synthesis in inflamed tissue and the urinary excretion of PG metabolites.

Acetates↗

PubMed1980

[Effects of estrogen on fatty acid composition in rat uterine lipids (author's transl)].

Estrogen effects on fatty acid (FA) composition in rat uterine lipids after ovariectomy were investigated. FAs in uterine lipids increased after estrogen injection. Estrogen enhanced the level of FA in phosphatidylethanolamine. The moieties of palmitic, palmitoleic, oleic and linoleic acids increased transiently more than twofold, 6 hours after estrogen administration and stearic and arachidonic acids increased to about two-fold 48 hours after this injection. Each FA measured increased 1.7 to 2.4 fold in phosphatidylcholine and total lipid, 48 hours after estrogen injection. Therefore, estrogen increases the production of phospholipids and has a considerable influence on the pattern of FA content in phosphatidylethanolamine. Membrane conformation in uterine cells is thus affected.

Animals↗

PubMed1980

[Influence of methyl 0-(4-hydroxy-3-methoxy-cinnamoyl) reserpate (CD-3400) on the gonadal and adrenal functions in rat (author's transl)].

Influence of CD-3400, which has been developed as a new antihypertensive agent in the class of rauwolfia alkaloids, was studied and effects were compared to those seen with reserpine. A single, oral administration of CD-3400 in a dose of 50 mg/kg to female rats had no effects on ovarian estradiol levels in contrast to the significant decrease at 6 and 18 hr seen after reserpine administration. CD-3400 given in a dose of 50 mg/kg to female rats increased to a lesser degree the adrenal corticosterone level at 1 hour than did reserpine. While the serum corticosterone level in the female rats was not changed with CD-3400 administration, a large increase was detected in the case of reserpine. Administration of CD-3400 or reserpine in a dose of 100 mg/kg to male rats produced no change in the testicular testosterone level, but in both cases induced a significant decrease in the testicular estradiol levels. This inhibition was higher in the case of reserpine treatment. In addition, the serum corticosterone level in male rats treated with reserpine was higher than levels after administration of CD-3400. Our results indicate that CD-3400 exerts a weaker effect than does reserpine on gonadal and adrenal functions of both sexes of rats.

Adrenal Glands↗

PubMed1980

[Biological assay of prostaglandin in rat urine using fundus of rat stomach (author's transl)].

Male Wistar rats, weighing about 180 g, were used. Strips from the fundus of the stomach were prepared from animals deprived of food for 24 hours and fed for 10 minutes before the decapitation. The strips were suspended in 2 ml of organ bath containing Tyrode solution kept at 30 degrees C, and the isometric contraction was determined. Prostaglandins in rat urine were separated by column chromatography and determined by biological assay. No less than 50 pg of PGE2 and 250 pg of PGF2 alpha with good precision could be determined quantitatively using this particular bioassay. Excretion of urinary PGE in the healthy male rat was 29+8+/-1.79 ng/day and PGF was 1.9+/-0.27 ng/day.

Animals↗

PubMed1979

Effect of intra-uterine administration of indomethacin on ovarian steroids.

Proteolytic enzymes increase the sensitivity of the bronchial system against acetylcholine when inhaled as aerosols. These experiments were performed on 33 dogs in order to localize the area of sensitivity in the bronchial system responsible for bronchoconstriction. Pronase given in a small dosage as proteolytic enzyme aerosol only to the upper part of the airtract sensitises the lower part of the airways against acetylcholine. Pronase aerosol given to the lower part of the airways needs higher dosages for sensitization to acetylcholine. This effect assumes an interaction between the upper sensoric part in the reflex way of airway obstruction and the lower airways. Changes caused by proteolytic enzymes in the upper part of the airtract can cause increased reactivity of the lower airways.

Animals↗

PubMed1979

[Influence of carrageenin inflammation on prostaglandin release from rat synovial membrane and action of anti-inflammatory drugs (author's transl)].

Carrageenin was injected into the knee joint cavity of rats, and influence of anti-inflammatory drugs on prostaglandin (PG) release in vitro from inflamed synovial membrane was studied. Release of PGE and F from inflamed synovial membrane was increased. Aspirin suppressed PGE and F release from non-inflamed and inflamed synovial membrane in vivo and in vitro, but suppression of PGF release was more intense. Hydrocortisone suppressed release of PGE and F from inflamed synovial membrane in vivo, and did not influence PG release in vitro. Release of PGE and F fron non-inflamed synovial membrane was not influenced by hydrocortisone in vivo and in vitro. PGE content in inflamed synovial membrane was increased markedly, and PGE and F content in inflamed and non-inflamed synovial membrane was decreased markedly by aspirin in vivo. Content of PGE and F in non-inflamed and inflamed synovial membrane showed a tendency to decrease with hydrocortisone administration in vivo, but such was not significant.

Animals↗

PubMed1977

Effect of estradiol on prostaglandin metabolism in rat uterus.

The purpose of this work was to investigate the effect of a single injection of estradiol (10 microng/rat) on prostaglandin(PG) metabolism in rat uterus. The PG content of uterus was increased within 6 hr after estradiol injection but the incorporation of 3H-arachidonic acid into PG in uterus was not changed within same period. The uptake of 3H-PG was increased within 6 hr after estradiol injection. In these results, it is discussed that PG from other tissue are also involved in the metabolism of PG in uterus.

Animals↗

PubMed1977

[Effect of estrogen on interconversion of prostaglandin F and E in rat uterus (author's transl)].

To determine estrogen action in PG metabolism in rat uterus, effect of the hormone on the uptake and interconversion of PGF and E was studied using 3H-PGF2alpha and 3H-PGE2. The following results were obtained: Uptake of 3H-PGF2alpha and 3H-PGE2 was increased at 6 hours after estrogen injection. The conversion into PGF and PGE group from 3H-PGE2 and 3H-PGF2alpha, respectively, was observed in rat uterus, but the hormone action on the interconversion of PG was apparently different between PGF2alpha and PGE2. The conversion of 3H-PGF2alpha into PGE group was increased with the lapse of time after estrogen injection. However, estrogen action on the conversion of 3H-PGE2 into PGF group was not so remarkable as that of 3H-PGF2alpha. Effects of indomethacin in vivo and in vitro on the metabolism of PGF2alpha increased by estrogen were also examined. Estrogen action on the uptake of PGF group was inhibited by indomethacin injection, but the conversion into PGE was not influenced by this compound.

Animals↗

PubMed1974