Search PubMed⌕ Search

Biomedical subjects

H Koffler

Publications and source records attributed to H Koffler.

35 records · Page 2Linked to original sources
PubMed1966

Detection and isolation of new glycosides in culture broths of Streptomyces hygroscopicus.

Elbein, A. D. (Purdue University, Lafayette, Ind.) J. A. Holowczak, Henry Koffler, and H. R. Garner. Detection and ìsolation of new glycosides in culture broths of Streptomyces hygroscopicus. J. Bacteriol. 91:604-608. 1966.-Four new compounds not previously reported have been isolated and purified from the culture broths of Streptomyces hygroscopicus. The data presented suggest that these compounds may be structurally related to hygromycin A, having similar aglycons but differing in their sugar moieties. Two of the compounds apparently contain d-glucose or l-fucose as their sugar components. The others, like hygromycin A, form crystalline 2,4-dinitrophenylhydrazones, and may contain unusual keto sugars.

Chromatography, Paper↗
PubMed1966

Attachment and structural features of flagella of certain bacilli.

Abram, Dinah (Purdue University, Lafayette, Ind.), A. E. Vatter, and Henry Koffler. Attachment and structural features of flagella of certain bacilli. J. Bacteriol. 91:2045-2068. 1966.-The attachment of flagella to cells of various mesophilic and thermophilic strains of Bacillus was studied electron microscopically. Studies of ghost cells and membrane fragments indicate that flagella are connected to the cytoplasmic membrane. Flagella removed from cells mechanically, during autolysis, or by phage lysis, have attached to the base of their proximal hooks material that is heterogeneous in character. In part, this material consists of cytoplasmic membrane; its varied shape appears to be caused by the folding of the membrane around the proximal end of the flagellum at the site of attachment. It is uncertain whether this material represents a real structure or an artifact. Highresolution microscopy reveals differences in the fine structure of intact flagella of the various strains studied. The proximal hook and the flagellar filament are distinct in morphology and fine structure. A specialized structure is associated with the hook of flagella of B. brevis and B. circulans. The filament of flagella of B. stearothermophilus 2184 has two regions that show marked differences in the manner in which the subunits appear to be organized. No correlation was found between the site of origin of flagella and the location of reduced tellurite when the reduction of potassium tellurite was used to indicate the loci of enzymatic respiratory activities.

Bacillus↗
PubMed1965

Basal structure and attachment of flagella in cells of Proteus vulgaris.

Abram, Dinah (Purdue University, Lafayette, Ind.), Henry Koffler, and A. E. Vatter. Basal structure and attachment of flagella in cells of Proteus vulgaris. J. Bacteriol. 90:1337-1354. 1965.-The attachment of flagella to cells of Proteus vulgaris was studied electron microscopically with negatively stained and shadow-cast preparations of ghosts from standard cultures and from special cultures that produced "long forms." The flagellum, the basal portion of which is hooked, arises within the cell from a nearly spherical structure, 110 to 140 A in diameter. This structure appears to be associated with the cytoplasmic membrane; it may be a part of the membrane or a separate entity that lies just beneath the membrane. Flagella associated with cell walls free from cytoplasmic membrane frequently have larger bodies, 200 to 700 A in diameter, associated with their base. These structures probably consist at least partly of fragments of the cytoplasmic membrane, a portion of which folds around a smaller structure. Flagella in various stages of development were observed in long forms of P. vulgaris cells grown at low temperature. The basal structure of these flagella was similar to that of the long or "mature" flagella. Strands connecting the basal structures were observed in ghosts of long forms; these strands appear to be derived from the cytoplasmic membrane. Flagella were found to be attached to fragments of cell wall and to cytoplasmic membrane in a similar manner as they are attached to ghosts. In isolates of flagella that have been separated from the cells mechanically, the organelles often terminate in hooks which almost always appear naked, but have a different fine structure than the flagellum proper.

Flagella↗
PubMedDate not supplied

Innovations in neonatal case management: an integrated, data-driven approach.

The intent of this article is to provide one company's perspective on the challenging and complex care management of the high-risk neonate. The strategies presented herein should enable and encourage case managers to implement an integrated management process for the frail neonatal population.

Case Management↗