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Biomedical subjects

H Klein

Publications and source records attributed to H Klein.

At least 271 records · Page 15Linked to original sources
PubMed1983

Therapeutic cytapheresis in chronic lymphocytic leukemia.

The role of therapeutic reductive lymphapheresis in 59 patients with chronic lymphocytic leukemia (CLL) was reviewed. Reduction in lymphocytosis, lymphadenopathy and hepatosplenomegaly was noted in 50%-60% of the patients studied. In addition to mobilization of the lymphoid tumor load, 40% of the patients showed an improvement in their hemogram. Although apheresis-induced anemia can occur, reported complications were minimal. These studies suggest that a majority of CLL patients might benefit from therapeutic lymphapheresis.

Aged↗
PubMed1983

Binding and effects of 5 alpha-androstane-3 beta,17 beta-diol in the male rat pituitary.

5 alpha-androstane-3 beta,17 beta-diol (Adiol) binds to cytosol proteins from male rat pituitary with a relatively high affinity (KD = 15 +/- 6 nM) and a low capacity (n = 92 +/- 8 fmol/mg protein). These saturable proteins which bind Adiol are characterized as estrogen receptor. This conclusion was based on the binding characteristics, the binding stereospecificity and the sedimentation coefficient in sucrose linear gradients. Moreover. Adiol induces, in vivo, the nuclear translocation of estrogen receptor and some effects of estrogen action. It is efficient to induce progesterone receptor and to increase pituitary protein content but inefficient to increase DNA synthesis. Results suggest a mechanism of Adiol action in the male rat pituitary similar to that observed with androgens in other target tissues. Moreover, the study of Adiol and 17 beta-estradiol binding suggests two forms of estrogen receptor in the cytosol from male rat pituitary. The maximal concentration of binding sites was observed at 22-30 days of age for E2 and at 37-42 days of age for Adiol. On the other hand, the nuclear ontogenic pattern suggested a single class of binding sites for E2 and Adiol in the pituitary nuclei.

Androstane-3,17-diol↗
PubMed1983

[Hemodynamics, plasma catecholamine behavior and beta-adrenergic receptor density in trained and untrained subjects and cardiac insufficiency patients].

Swan-Ganz semifloating balloon-tipped catheters were introduced in 6 endurance-trained subjects, 7 untrained volunteers, 29 patients suffering from coronary heart disease, 8 patients with right heart insufficiency induced by chronic obstructive syndromes, and 8 patients with idiopathic congestive cardiomyopathy. All subjects except the patients with resting cardiac insufficiency performed graded ergometric tests during the catheter investigation. Hemodynamic values, plasma noradrenaline and adrenaline (in all subjects), and beta-adrenergic receptor density on intact polymorphonuclear leucocytes (in 6 endurance-trained subjects, 5 untrained healthy volunteers, and 6 patients with left heart insufficiency) were determined. For all subjects investigated, significant correlations were observed between resting plasma catecholamine levels and resting hemodynamic values, such as stroke volume (r = 0.47, p less than 0.001), cardiac output (r = 0.32, p less than 0.05), heart rate (r = 0.37, p less than 0.01), pulmonary vascular resistance (r = 0.52, p less than 0.001), and total vascular resistance (r = 0.40, p less than 0.01). An inverse relationship existed between the resting catecholamine levels and the performance ability. Catecholamine levels were approximately three- to fourfold higher and the beta-adrenergic receptor density approximately two- to threefold lower in patients with left heart insufficiency than in healthy untrained subjects. The plasma catecholamine levels were lower and the beta-adrenergic receptor density approximately 60% greater in trained subjects. The beta-adrenergic receptor density may be a critical dynamic parameter for the modulation of sympathetic effects.

Adult↗
PubMed1983

[Results of electrophysiologically guided surgery in ventricular tachycardia].

We report on 33 patients (pts) with medically refractory ventricular tachycardia (VT) after myocardial infarction in whom electrophysiologically guided surgery was performed. In 18 pts the origin of the VT was identified preoperatively by endocardial catheter mapping. Intraoperative endocardial mapping was performed in 32 pts. The origin of the VT was detected in 24 pts, whereas in 8 pts the surgical procedure was guided by the results of intraoperative mapping during sinus rhythm. In one patient no intraoperative mapping was performed and surgery was guided only according to the results of endocardial catheter mapping. A deep endomyocardial incision was performed in 14 pts, whereas local endocardial resection was done in 19 pts. Six pts (18%) died postoperatively still being in the hospital, 27 pts (82%) were discharged. There were 4 late deaths during mean follow up period of 18 months. One patient had a spontaneous recurrence of VT. 8 pts remained on antiarrhythmic medication due to complex ventricular arrhythmias. We conclude that in case of medically refractory VT in coronary artery disease, surgical therapy becomes an acceptable alternative when guided by pre- and intraoperative electrophysiological mapping.

Adult↗
PubMed1982

Treating Israeli Soldiers in a civilian psychiatric setting.

The structure, functioning, and treatment concept of a psychodynamically oriented psychiatric unit for young soldiers within Israeli civilian hospital are discussed stressing: (1) the integration of the patient's military self; (2) the resolution of psychological conflicts necessary to avoid traumatic developmental arrest of the patient's personality and to foster personality growth; (3) the involvement of the patient's family in the therapeutic process. Problems in the patient-therapist relationship specifically pertaining to Israeli conditions are outlined.

Adolescent↗
PubMed1982

O2 consumption by isolated pancreatic islets, as measured in a microincubation system with a Clark-type electrode.

The role of B-cell respiration in fuel-induced insulin secretion has not been clarified. Therefore, a new method for the measurement of O2 uptake in islets of Langerhans was developed. An all-glass microincubation chamber was equipped with a Clark-type electrode, a stirring bar, and a special channel for loading the chamber with islets, media, and test compounds. The sensitivity of the system was sufficient for convenient determination of O2 consumption by less than 100 islets. Using DNA as reference value, the exactness of the method was scaled up considerably. Basal O2 uptake in mouse islets amounted to 5.6 +/- 0.2 nmol/h/micrograms DNA. alpha-Ketoisocaproic acid (2.5-20 mM) enhanced O2 consumption by 63-207%. The rate of O2 uptake as well as those of insulin secretion and oxidation of alpha-ketoisocaproic acid in incubated mouse islets were maximal at about 10 mM alpha-ketoisocaproic acid. 14CO2 production from U-14C-labeled alpha-ketoisocaproic acid was up to 36% lower than the corresponding increase in O2 uptake. However, the differences were partly caused by insufficient mixing of media in the oxidation studies. D-Glucose (20 mM) released more than twice the amount of insulin than 5 mM alpha-ketoisocaproic acid, although O2 uptake in the islets did not differ. The results are consistent with the view that an increase in the production of metabolic energy is necessary for recognizing insulin-releasing fuels by B-cells.

Animals↗
PubMed1982

[Qualitative and quantitative determination of procaine-, lidocaine- and tetracaine hydrochloride in drugs by means of capillary isotachophoresis].

The author describes a method for the qualitative and quantitative simultaneous determination of procaine-, lidocaine- and tetracaine hydrochloride in drugs. These active principles are separated by isotachophoresis on the basis of differences in their net mobilities. It took 12 min per sample to perform this analytical technique and the rate of recovery has a mean value of 99.7%. Acids, vitamins and nonionized organic compounds present in the drugs do no interfere with the isotachophoretic separation.

Anesthetics, Local↗