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Biomedical subjects

H Kitagawa

Publications and source records attributed to H Kitagawa.

At least 397 records · Page 22Linked to original sources
PubMed1988

Bromocriptine treatment in anovulation with decreased ratio of follicle stimulating hormone to luteinizing hormone and with hyperandrogenism.

Twenty-six anovulatory women of polycystic ovarian (PCO)-type were treated with bromocriptine (Br) at a daily dose of 5 mg for 2 months. Ovulatory cycles were resumed in 18 (69.2%) women (Br-responders). No difference between pretreatment serum levels of FSH, LH, PRL and estradiol and FSH/LH ratios in Br-responders and nonresponders was observed. The geometric mean of circulating androstenedione (A-dione) in Br-responders (2.58 ng/ml) appeared higher than that in nonresponders (2.11 ng/ml) but was not statistically significant. The geometric mean of dehydroepiandtrosterone sulfate (DHEA-S) in Br-responders (1652 ng/ml) was lower (p less than 0.01) than that in nonresponders (2582 ng/ml). The ratio of DHEA-S to A-dione (D/A ratio) exhibited a highly significant between-group difference (p less than 0.001) (646 and 1222 for Br-responders and nonresponders, respectively). Br-nonresponders with high DHEA-S levels and D/A ratios tended to hyperproduction of adrenal androgen, and Br-responders with high A-dione levels and low D/A ratios to hyperproduction of ovarian androgen. The present study indicates that Br is effective in PCO-type women presumably with ovarian androgen hyperproduction. The efficacy of Br, when applied to PCO-type women, could be predicted with their D/A ratios.

Adult↗
PubMed1988

Ethyl eicosapentaenoate restored the immunosuppression in mice fed fat-free diet.

The effects of eicosapentaenoic acid (EPA) on the humoral immune response in fat free diet-fed (FF) mice were studied. The lowered anti-SRBC PFC activity of ICR male FF mice was restored in a dose-dependent manner when EPA was administered orally at doses of 60-360 mg/kg/day for 20 days. In in vitro experiments, EPA similarly enhanced anti-SRBC PFC activity but did not affect the response against lipopolysaccharide. Furthermore, EPA did not cause any substantial effect on T suppressor cell activity induced by Concanavalin A in vitro. On the other hand, T helper cell activity induced by keyhole limpet hemocyanin was augmented. From these results, it is suggestive that EPA caused immunopotentiation to FF mice at least partially by an enhancement of T helper cell activities.

Animals↗
PubMed1988

Purification and some properties of NADPH-cytochrome P-450 reductase from crab-eating monkey liver microsomes.

NADPH-cytochrome P-450 reductase was purified to 30.8 units/mg from monkey liver microsomes. The purified reductase showed one major protein band (78,000) and two minor ones (58,000 and 20,000) on analysis by SDS-polyacrylamide gel electrophoresis (SDS-PAGE). Monkey, rat, and guinea pig reductases were not immunochemically identical to each other judged from Ouchterlony double diffusion analysis and immunotitration with regard to NADPH-cytochrome c reductase activity.

Amino Acids↗
PubMed1988

Effects of ketoprofen and other drugs on release of prostaglandins from uterus of rabbits in perinatal period.

The effects of oral administration of ketoprofen, naproxen, flurbiprofen (1.0 mg/kg), indomethacin (1.0 and 3.0 mg/kg), and acetylsalicylic acid (100 mg/kg) on release of prostaglandin F2 alpha (PGF2 alpha) and prostaglandin E (PGE) from isolated pregnant rabbit uterus were studied comparatively. Ketoprofen, indomethacin, flurbiprofen, naproxen and acetylsalicylic acid reduced the release of PGF2 alpha and PGE from the isolated rabbit uterus. The inhibitory effect was most profound with flurbiprofen, followed, in order, by naproxen, ketoprofen, indomethacin and acetylsalicylic acid. A comparative study with intramuscular ketoprofen (0.1, 1.0 and 3.0 mg/kg) was also carried out. The inhibitory effect of ketoprofen on uterine PGF2 alpha and PGE release with intramuscular route was about 10 times more profound than that with oral route.

Animals↗
PubMed1988

Glutathione depletion by 2-nitroimidazole-1-acetohydroxamic acid as a radiosensitizer in hypoxic rat hepatocytes.

The effects of nitroimidazoles as radiosensitizers on intracellular glutathione (GSH) level were investigated in rat isolated hepatocytes. Dinitroimidazoles have lowered almost completely GSH level during the incubation for 30 min under oxic (95% O2+5% CO2) condition, while mononitroimidazoles had scarcely affected. In the case of hypoxic (95% N2+5% CO2) condition, however, 2-nitroimidazoles, not 4-nitroimidazoles, as well as 2,4- and 4,5-dinitroimidazoles have caused the significant depletion of GSH. This suggests that nitro group in the 2-position of imidazoles may be responsible for the GSH depletion under hypoxia. Especially, 2-nitroimidazole-1-acetohydroxamic acid (KIH-801) was found to be the most potent GSH depletor only under hypoxic, not oxic conditions, and might be useful for the new hypoxic cell radiosensitizer instead of misonidazole.

Animals↗
PubMed1988

Polyamine lowered the hepatic lipid peroxide level in rats.

This is the first report for the hepatic lipid peroxide lowering effect of spermine in vivo. The influence of administration of polyamines on hepatic lipid peroxide level has been investigated by using normal or carbon tetrachloride (CCl4)-treated rats. Spermine was found to lower the hepatic lipid peroxide level most efficiently among polyamines used in CCl4-treated rats. In addition, the extent of liver enlargement caused by CCl4 treatment was reduced by spermine administration. Lipid peroxide lowering effect of spermine was also observed in normal rats. Hepatic spermine content was significantly increased in both normal and CCl4-treated rats after administration of spermine. Clear inverse relationship between the content of lipid peroxide and the concentration of spermine was observed. In reconstituted system containing NADPH-cytochrome P-450 reductase and extracted hepatic microsomal lipid, spermine inhibited the NADPH-dependent lipid peroxidation effectively at the concentration of 0.1 mM. From these results, we concluded that spermine exerted an inhibitory effect of lipid peroxidation in vivo as well as in vitro.

Animals↗
PubMed1988

Studies on murine IgE with monoclonal antibodies. I. Characterization of rat monoclonal anti-IgE antibodies and the use of these antibodies for determinations of serum IgE levels and for anaphylactic reactions.

Rat monoclonal antibodies were constructed by fusion of immunized rat spleen cells with a nonsecreting mouse myeloma cell. Two monoclonal antibodies (6HD5 and HMK-12) were selected for further study. Both reacted with various IgE molecules of different specificities and different allotypes, but did not react with immunoglobulins of other isotypes and with light chains. These antibodies were therefore anti-isotypic (IgE) and not anti-allotypic or anti-idiotypic. It was shown by competition studies that these antibodies recognize different epitopes on the FcR epsilon fragment. A sensitive ELISA for the quantitation of murine IgE was developed with these monoclonal antibodies; the sensitivity was between 2 and 250 ng/ml for detection of serum IgE levels. Good correlation was obtained with protein amounts as determined by enzyme-linked immunosorbent assay (ELISA) and passive cutaneous anaphylaxis (PCA) activities. Both monoclonal antibodies were used to study anaphylactic reactions elicited by IgE antibodies. Both could inhibit PCA reactions and both could elicit reverse PCA reactions.

Animals↗
PubMed1988

[Drug interaction between imipramine hydrochloride and benzodiazepines when administered orally for 15 days].

The effects of daily oral administration of imipramine (IM) hydrochloride (50 mg/kg) and/or oxazepam (OZ, 20 mg/kg) or diazepam (DZ, 5 mg/kg) in a 1% aqueous solution of carboxymethylcellulose sodium salt (CMC) on the activities of drug metabolizing enzyme systems and steady state plasma levels of IM, desmethylimipramine (DIM), DZ, desmethyl-diazepam (DDZ) and OZ were investigated in rats during a 15-day period. In addition, the effect of a single intravenous administration of IM hydrochloride (5 mg/kg), DZ (0.5 mg/kg) and OZ (2 mg/kg) on plasma concentration-time profiles of IM, DIM, DZ, DDZ and OZ was investigated in rats given the same drug treatments by the oral route for 14 days. The group treated with IM hydrochloride plus OZ group showed a great increase in drug-metabolizing enzyme activities, but the difference for the group given IM hydrochloride was not statistically significant. The steady-state plasma levels of DZ after oral administration for 15 days suggested that the group given IM hydrochloride plus DZ did show accumulated DZ. In terms of the area under the concentration-time curve of IM, DZ and OZ after intravenous administration of IM hydrochloride, DZ and OZ, there were significant differences between each of the mono-treatment groups and the IM hydrochloride plus DZ or OZ treatment groups. In conclusion, we have found that the drug interaction for IM hydrochloride by OZ is markedly lower than that by DZ.

Administration, Oral↗