In October 1982 two patients suffering from paraplegia ware, world wide for the first time, implanted two 8-channel-stimulation-devices at the II. Surgical Clinics Vienna. Four electrodes were fixed to the nervus femoralis and the nervus gluteus inferior with the help of microsurgical techniques. The aim of the operation is to enable the paralysed patient to "stand up himself" and to "cover a short distance on crutches". They must learn to manipulate the implanted "more-channel-device" with their fingers, and to coordinate this with the supporting function and the balance function of their arms. The wheel-chair shall not and cannot be substituted with this method: Not without, but with wheel-chair and functional electrostimulation. A comprising training-programme for building up muscles, balance control and gait training was necessary. After 4 months muscle-force and endurance had increased 200%. The patients could stand up stimulatedly without help between parallel bars. At the rehabilitation center Bad Häring the first steps were done in March 1983 (= 5. month after operation). The greatest success was when a patient could get stimulatedly out of her car and do the through-swing-gait over a distance of 60 meters. After one year the muscle force was quadrupled, the endurance was increased 20-30 times, depending on the type of gait. Both patients are able to walk in the 4-P-gait or swing-through-gait on crutches over short distances without help from others. Riding a bicycle stimulatedly on even ground was demonstrated.

Adult↗

PubMedDate not supplied

Complex formation among rat pancreatic secretory proteins under mild alkaline pH conditions.

Previous in vitro studies have demonstrated that enzyme proteins liberated from isolated zymogen granules of the rat pancreas aggregate already at neutral or slightly basic pH and form small particles which in the acidic pH range progressively condense into dense cores of about the size of zymogen granules. To characterize the protein composition of the original particles in more detail non-denaturing agarose gel electrophoresis was employed. Five major protein complexes were identified which upon separation of individual complexes in 1-D or 2-D gel electrophoresis were shown to be composed of a distinct set of known enzymes and several unknown proteins. Complexes 1-4 quickly dissociated when enzyme activation was induced by enterokinase, but complex 5 was resistant even to this treatment. All 5 complexes revealed a distinct fine structure when eluted from the gels and studied in negative staining electron microscopy. These findings suggest that pancreatic zymogens form complexes already in the lumen of the rough endoplasmic reticulum and are transported as such to the Golgi complex where they aggregate into granule cores due to the internal acidic pH. Complex formation may thus facilitate zymogen sorting within the rough endoplasmic reticulum and may prevent premature enzyme activation within cellular compartments.

Animals↗

PubMedDate not supplied

[Influence of immobilized ribonuclease on the cell-free protein synthesis in the wheat system (author's transl)].

In order to increase the efficiency of different mRNAs from wheat seedlings in carrying out cell-free protein synthesis in the wheat system, efforts were made to remove endogenous mRNA. In this direction, we checked the possibility of using immobilized RNase. Treatment of the cell-free system or its components with this enzyme caused a large decrease in the efficiency of poly tu-directed incorporation of labeled amino acids. This effect did not coincide with an equivalent degradation of RNA, as has been shown by analysis of ribosomes and polysomes. The results are discussed in the light of recent findings of some authors.

Cell-Free System↗

PubMedDate not supplied

[CNA-synthesis in gamma-platlets of wheat (author's transl)].

Plants, arising from irradiated (300 kR) caryopses of wheat, exhibit a low incorporation of [3H]thymidine into the DNA, which, as shown by sedimentation on sucrose gradients, does not indicate tendencies of normalization.

DNA↗

PubMedDate not supplied

[Affinity chromatography with 125I-labeled RNA].

Iodination of RNA with carrier-free 125J changes the affinity to cellulose and polyuridylic acid. Because part of the radioactive RNA is tenaciously bound to the respective matrix [125J[RNA cannot be used for affinity chromatography.

Chromatography, Affinity↗

PubMedDate not supplied

[Redundant nucleotide-sequences in DNA from wheat (author's transl)].

DNA from dormant and germinated embryos exhibit differences in the kinetics of reassociation of thermal denatured nucleic acid. Within 24 h of germination a reduction of the renaturation, expressed as Cot1/2 of the redundant fraction, is detectable.

Base Sequence↗