Living hepatocytes observed under standard methods of cell isolation and culture show frequent active contractions of bile canaliculi when viewed by time-lapse cinephotomicrography. This report gives details of the methods used to show the contractions. In addition, the characteristics of the contraction pattern are defined and analyzed. The contractions are regular, canalicular contraction time (systole) lasts 60 sec, and the most probably interval between contractions is 5 1/2 min. The finding of a regular pattern of contraction suggests an ordered, programmed, and integrated mechanism which entails a high degree of communication and functional coordination between neighboring hepatocytes.

Animals↗

PubMedDate not supplied

A technic of general anesthesia for blepharoplasty and rhytidectomy.

A series of 66 consecutive cases of blepharoplasty and rhytidectomy are reported, 60 of which were performed with a general anesthesia technic utilizing local anesthesia with epinephrine, in association with enflurane inhalation delivered via bilateral nasopharyngeal airways (BNPA). Six cases were not suitable candidates for the technic because of physical status or for anatomic reasons. The technic has teh advantage of providing airway control without tracheal intubation, light depth of anesthesia, compatibility with epinephrine, and highly acceptable limits of intraoperative bleeding and postoperative hematoma.

Adult↗