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Biomedical subjects

H Katsunuma

Publications and source records attributed to H Katsunuma.

At least 55 records · Page 3Linked to original sources
PubMed1985

Quantitative cytochemical differences between young and old patients with lung cancer.

In order to study differences in the tumor growth and frequency of metastases between younger and older patients with lung cancer, we investigated the nuclear DNA and nuclear protein according to age by means of cytophotometry after combined staining with Feulgen and Naphthol Yellow S. On the basis of Feulgen-Naphthol Yellow S staining method, 13 patients less than 40 years old and 16 patients older than 70 years were investigated. Our results showed no significant difference in nuclear DNA contents between young and old patients, but there were significantly higher nuclear protein contents (p less than 0.05) and nuclear protein to nuclear DNA (NP/DNA) ratios (p less than 0.001) in young patients than in old patients. This suggests that young patients may have higher tumor proliferation (high nuclear protein contents and NP/DNA ratios). The lower nuclear protein content and NP/DNA ratio of older cases is in keeping with the general phenomenon of slower tumor growth and less frequent metastases in such cases.

Adenocarcinoma↗
PubMed1983

Anti-atherosclerotic action of elastase--with special reference to its effect on elastic fibres.

In order to determine the anti-atherosclerotic action of elastase, the aortas from four groups of rats--group N fed a normal diet, group D fed an atherogenic diet with vitamin D2, group N + Ela fed a normal diet with elastase, and group D + Ela fed an atherogenic diet and vitamin D2 with elastase--were studied histologically after 3, 6, 9, and 12 weeks of feeding. Group D displayed a remarkable atheromatous change, while the change was insignificant in group D + Ela. The histopathological findings in group D consisted of marked rarefaction, fat deposition, and atheroma in the subendothelial tissue and tunica intima; Van Gieson's staining revealed a conspicuous decrease in elastic fibres, and the remaining elastic fibres had such abnormalities as a tortuous course, bends, and fissures. For group D + Ela, on the other hand, there were few foam cells in the subendothelial tissue and tunica intima and much less marked rarefaction of interstice and fat deposition. Further, the arrangement of elastic fibres was only slightly disordered, and the decreases in elastic fibres and their fissures were moderate. In sharp contrast to group D. which showed marked calcium deposition, group D + Ela was found to have low calcium deposition. No macroscopic and histopathologic abnormalities were found in groups N and N + Ela. Elastase had an inhibitory effect on experimental atherosclerosis in the rat and also regulated the degradation and biosynthesis of elastin, which resulted in the regeneration of elastic fibres.

Animals↗
PubMed1983

Early appearance and localization of intranuclear inclusions in the segments of renal proximal tubules of rats following ingestion of lead.

Intranuclear inclusions in epithelial cells lining rat renal proximal tubules were detected by electron microscopy as early as 4 days after the addition of lead (5 mg/ml as lead acetate) to the drinking water. At 9 and 12 weeks pathological changes, but very few intranuclear inclusions, were apparent in the epithelial cells lining the third segment of the proximal tubules in the outer stripe of outer zone of the medulla. On the other hand, morphological changes were less in the epithelial cells lining the first and second segments of the proximal tubules in the cortex, which contained many intranuclear inclusions. These findings suggest that lead incorporated into the epithelial cells in the proximal tubules may exist in an inert chemical form as inclusions, especially in the second segment. These bodies, therefore, may protect the cells from the toxic effect of lead.

Animals↗