Search PubMed⌕ Search

Biomedical subjects

H Kaneshige

Publications and source records attributed to H Kaneshige.

At least 37 records · Page 2Linked to original sources
PubMed1983

Impaired solubilization of glomerular immune deposits by sera from patients with IgA nephropathy.

A study of the solubilization of glomerular immune deposits by sera from patients with IgA nephropathy is described. Renal biopsy specimens were obtained from patients with IgA nephropathy and other glomerular diseases. These specimens were incubated with fresh and heated sera from the same patients and healthy adults at 37 degrees C for one hour in plastic tubes. The sections were stained with fluorescein isothiocyanate (FITC)-labeled heavy chain specific anti-human IgA antiserum and then examined with a fluorescent microscope. It was shown that the solubilization of glomerular immune deposits by sera from patients with IgA nephropathy was significantly less than that by sera from healthy adults. It is possible that impaired solubilization of immune complexes in vivo could lead to the accumulation of glomerular immune deposits in patients with IgA nephropathy.

Antigen-Antibody Complex↗
PubMed1983

A case of Fabry's disease.

A case of Fabry's disease in a 22-year-old male patient who had mild proteinuria and dark-red eruptions is reported. He had been treated as a case of a so-called "chronic glomerulonephritis" for one year. However, histopathological findings of the renal biopsy specimens showed the presence of numerous vacuolated cells in the glomeruli. These vacuolated cells contained numerous electron dense bodies observed by electron microscopy. Skin lesions of this patient were consistent with those of angiokeratoma corporis. The levels of serum alpha-galactosidase were significantly lower than those of healthy controls. The mother of this patient also showed decreased levels of serum alpha-galactosidase. The pedigree of this patient showed a familial history of various types of renal diseases. It was postulated that Fabry's disease occurring in older patients has a worse clinical course. it is concluded that early detection of this disease through biopsy and the assay of serum alpha-galactosidase levels is important in managing the future course of patients with Fabry's disease.

Adult↗
PubMed1983

Cross-reactivity of IgA antibodies between renal mesangial areas and nuclei of tonsillar cells in patients with IgA nephropathy.

A study on autoradiographical analysis of antigenic sites in patients with IgA nephropathy is described. Renal biopsy specimens were obtained from patients with IgA nephropathy. These specimens were treated with citrate buffer (pH 3.2) and the 'eluate' was neutralized by sodium hydroxide. The 'eluate' was labelled with 125iodine by the chloramine-T method. 125I-labelled eluate was then applied to the tonsillar cells obtained from the same and other patients with IgA nephropathy as well as to those with other glomerular diseases. The tonsillar cells were dipped into the emulsion (NBT-2) and then examined with a light microscope. It was demonstrated that the antibodies eluted from renal tissues of patients with IgA nephropathy specificially bound with the nuclear regions of tonsillar cells. The binding of eluted antibodies and tonsillar cells was completely inhibited by the addition of anti-human IgA antisera, but not inhibited by human IgA myeloma proteins. The eluted antibodies bound with tonsillar cells from the same patients, but only 10% of them bound with the tonsillar cells obtained from other patients with IgA nephropathy. It is concluded that IgA antibodies deposited in glomeruli specifically bind with tonsillar cells obtained from patients with IgA nephropathy and these antibodies show some heterogeneity among those patients.

Adult↗
PubMed1982

Determination of LDL receptors on cultured lymphocytes using fluorescein-labeled LDL.

A rapid, sensitive procedure for the detection of LDL receptors on cell surface without using radioisotopes is described. Fluorescein-isothiocyanate (FITC) labeled LDL bound with cultured human lymphocytes, and the degree of that binding was compatible with that of 125 I-labeled LDL. The staining of cells with FITC-LDL allows identification and enumeration of LDL-binding cells using fluorescence microscopy or a fluorescence activated cell sorter (FACS). It is suggested that FITC-LDL can be applied for screening of patients with various degrees of activity in LDL receptors on their cell surfaces.

Adult↗
PubMed1982

A case of IgA nephropathy associated with marked hematuria after upper respiratory tract infections.

IgA nephropathy is characterized by recurrent hematuria which is occasionally associated with upper respiratory infections. Since serial follow-up of IgA nephropathy has not been reported, a 43 year old patient who showed a typical course of this disease is described. Hematuria was markedly increased each time after upper respiratory infections. Renal biopsy revealed typical features of IgA nephropathy by light and immunofluorescent microscopy. Marked hematuria subsided after administration of antibiotics. Upper respiratory tract infections might be a risk factor in some patients with IgA nephropathy. In some patients upper respiratory tract infections may exacerbate IgA nephropathy.

Adult↗
PubMed1982

Prevalence of IgAl deposits in Henoch-Schoenlein purpura (HSP) nephritis.

A study on the detection of IgA1, IgA2 and the J("joining") chain in glomeruli from renal biopsy specimens in patients with Henoch-Schoenlein purpura (HSP) nephritis is described. Renal biopsy specimens from five patients with HSP nephritis were stained with FITC-labelled anti-human IgA1, IgA2 and J chain, and then examined with a fluorescent microscope. IgA1 was detected in glomeruli from all patients with HSP nephritis examined in this study. IgA2 was not observed in any patients. These findings were similar to those for IgA nephropathy reported previously. Histopathological findings of glomeruli in HSP nephritis were almost identical to those in IgA nephropathy. The levels of serum IgA in patients with HSP nephritis increased as observed in IgA nephropathy. It is suggested that there are some immunopathological similarities between HSP nephritis and IgA nephropathy.

Adult↗
PubMed1982

A case report of Henoch-Schoenlein purpura nephritis associated with a postbulbar duodenal ulcer.

We experienced an adult patient with Henoch-Schoenlein purpura (HSP) nephritis associated with a postbulbar duodenal ulcer. Chronic inflammatory changes of the arterioles (i.e. vasculitis) in the kidney and skin were prominently observed by immunofluorescence and light microscopic analysis. It is postulated that a postbulbar duodenal ulcer occurred in this patient and was mediated by vascular deposition of circulating immune complexes. It was suggested that HSP nephritis is a diffuse vascular form of IgA nephropathy.

Duodenal Ulcer↗
PubMed1982

Impaired granulocyte function in patients with diabetes mellitus.

Phagocytosis and intracellular killing of Staphylococcus aureus by granulocytes were examined in diabetic patients. There was no significant difference in the phagocytic activity of granulocytes between control and diabetic-subjects. However, intracellular killing by granulocytes was significantly reduced in insulin-treated diabetic patients compared with control subjects. No significant difference was observed between controls and diet-treated diabetic patients. It is suggested that decreased activity of intracellular killing of bacteria in granulocytes is one of the mechanisms of increased susceptibility to infection in patients with advanced stages of diabetes mellitus.

Diabetes Mellitus↗
PubMed1981

Aldosterone-producing microadenoma in a patient with primary aldosteronism.

A 54-year-old patient was found to have an aldosterone-producing microadenoma measuring 2.5 mm in diameter. Although adrenal venography by selective catheterization had failed to demonstrate the tumour, adrenal scintiscan using 131I-19-iodocholesterol accurately localized the functional tumour in the right adrenal gland; a dexamethasone-modified adrenal scintiscan revealed complete suppression of radioactivity in the right adrenal gland. These findings suggest that adrenal scintiscan, together with adrenal vein blood analysis, is capable of detecting a small adenoma before surgery, and that dexamethasone suppression of an adrenal scintiscan does not exclude an adenoma.

Adenoma↗
PubMed1981

IgA-specific helper activity of T alpha cells in human peripheral blood.

A subpopulation of human T lymphocytes that express receptors of immunoglobulin A (T alpha cells) was separated by a FACS using FITC-conjugated human IgA myeloma protein in order to determine whether they have helper activity for in vitro immunoglobulin synthesis in comparison with a T alpha cell population separated by rosette formation using PTN-ORBC conjugated with MOPC-315 mouse IgA myeloma protein. FACS-separated T alpha cells have IgA specific helper activity, whereas rosette-separated T alpha cells have polyclonal helper activity for in vitro immunoglobulin synthesis in PWM-stimulated B cells. The polyclonal helper activity observed in rosette-separated T alpha cells might be due to contamination of non-T alpha cells in the T alpha cell fraction. It is concluded that purified T alpha cells in normal human peripheral blood show IgA-specific helper activity in vitro.

Animals↗
PubMed1981

IgA nephropathy associated with myasthenia gravis and scleritis.

A 29-year-old male patient with IgA nephropathy developed scleritis and myasthenia gravis during the follow-up period. No lymphocytic infiltration or atrophy in muscle fibers was observed in muscle biopsy specimens. However, an immunofluorescent study on the same specimens showed deposits of immunoglobulin A in muscular vessels. The development of scleritis and myasthenia gravis in a patient with IgA nephropathy suggests a common immunologic basis for these disorders.

Adult↗
PubMed1981

Immunofluorescent studies on acute phase reactants in patients with various types of chronic glomerulonephritis.

A study on immunofluorescent staining of so-called "acute phase reactants" (haptoglobin, alpha 1-acid glycoprotein and alpha 1-antitypsin) and beta-lipoprotein in patients with various types of chronic glomerulonephritis is described. Eight patients with IgA nephropathy, eleven patients with proliferative glomerulonephritis, six patients with benign recurrent hematuria, four patients with membranous nephropathy and one patient with Henoch-Schönlein purpura nephritis (HSP nephritis) were examined. Deposition of haptoglobin was observed in patients with IgA nephropathy and HSP nephritis, and that of beta-lipoprotein was observed in various types of chronic glomerulonephritis. There was a significant correlation between the degree of deposition of haptoglobin and that of hematuria in patients with IgA nephropathy and HSP nephritis. It is suggested that deposition of haptoglobin and beta-lipoprotein may be related to "hemolysis" in glomeruli and increased permeability of glomerular capillary walls in kidneys with various types of chronic glomerulonephritis.

Chronic Disease↗
PubMed1981

Selective IgM deficiency: a case study.

We experienced an 85-year-old male patient with selective IgM deficiency. The average levels of his serum immunoglobulins were as follows: IgG, 1,165 mg/dl, IgA 280 mg/dl, and IgM 17 mg/dl. The subpopulations of peripheral blood lymphocytes in this patient were normal, including normal numbers of IgM-bearing lymphocytes. Normal amounts of cells with cytoplasmic IgM were induced by in vitro addition of pokeweek mitogen, and no inhibitory cells or inhibitory factors for in vitro production of IgM were demonstrated in his blood. These results suggest that some defects similar to those in patients with selective IgA deficiency may play a role in the development of selective IgM deficiency.

Aged↗
PubMedDate not supplied

Fibrinolysis in patients with diabetic nephropathy determined by plasmin-alpha 2 plasmin inhibitor complexes in plasma.

Activation of fibrinolysis in patients with diabetic nephropathy was determined by the plasma levels of plasmin-alpha 2 plasmin inhibitor complexes (alpha 2PIC) using a one-step sandwich enzyme immunoassay (EIA). Plasma levels of alpha 2PIC in diabetic patients with persistent proteinuria were significantly higher than those in diabetic patients without proteinuria, patients with chronic glomerulonephritis, and healthy adults. Plasma levels of alpha 2PIC in diabetic patients with intermittent proteinuria were also significantly higher than those of diabetic patients without proteinuria, patients with chronic glomerulonephritis, and healthy adults. Diabetic patients have been suggested to have a hypercoagulable state. The findings obtained from this study indicated that activation of fibrinolysis might counteract the hypercoagulable state in patients with diabetic nephropathy.

Adult↗