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Biomedical subjects

H K Huang

Publications and source records attributed to H K Huang.

At least 37 records · Page 2Linked to original sources
PubMed2000

Real-time teleconsultation with high-resolution and large-volume medical images for collaborative healthcare.

Real-time consultation between referring physicians or radiologists with an expert is critical for timely and adequate management of problem cases. During consultation, both sides need to: 1) synchronously manipulate high-resolution digital radiographic images or large volume MR/CT images; 2) perform interpretation interactively; and 3) converse with audio. We present a specifically designed teleconsultation system in a digital imaging and communication in medicine picture archiving and communication systems clinical environment. The system uses bidirectional remote control technology to meet critical teleconsultation application requirements with high-resolution and large-volume medical images operated in a limited-bandwidth network setting. We give the system design and implementation methods, and also describe the teleconsultation procedure and protocol used in this system. Finally, laboratory and clinical evaluation results are discussed.

Delivery of Health Care↗
PubMed1998

RNA polymerase I-promoted HIS4 expression yields uncapped, polyadenylated mRNA that is unstable and inefficiently translated in Saccharomyces cerevisiae.

The HIS4 gene in Saccharomyces cerevisiae was put under the transcriptional control of RNA polymerase I to determine the in vivo consequences on mRNA processing and gene expression. This gene, referred to as rhis4, was substituted for the normal HIS4 gene on chromosome III. The rhis4 gene transcribes two mRNAs, of which each initiates at the polymerase (pol) I transcription initiation site. One transcript, rhis4s, is similar in size to the wild-type HIS4 mRNA. Its 3' end maps to the HIS4 3' noncoding region, and it is polyadenylated. The second transcript, rhis4l, is bicistronic. It encodes the HIS4 coding region and a second open reading frame, YCL184, that is located downstream of the HIS4 gene and is predicted to be transcribed in the same direction as HIS4 on chromosome III. The 3' end of rhis4l maps to the predicted 3' end of the YCL184 gene and is also polyadenylated. Based on in vivo labeling experiments, the rhis4 gene appears to be more actively transcribed than the wild-type HIS4 gene despite the near equivalence of the steady-state levels of mRNAs produced from each gene. This finding indicated that rhis4 mRNAs are rapidly degraded, presumably due to the lack of a cap structure at the 5' end of the mRNA. Consistent with this interpretation, a mutant form of XRN1, which encodes a 5'-3' exonuclease, was identified as an extragenic suppressor that increases the half-life of rhis4 mRNA, leading to a 10-fold increase in steady-state mRNA levels compared to the wild-type HIS4 mRNA level. This increase is dependent on pol I transcription. Immunoprecipitation by anticap antiserum suggests that the majority of rhis4 mRNA produced is capless. In addition, we quantitated the level of His4 protein in a rhis4 xrn1delta genetic background. This analysis indicates that capless mRNA is translated at less than 10% of the level of translation of capped HIS4 mRNA. Our data indicate that polyadenylation of mRNA in yeast occurs despite HIS4 being transcribed by RNA polymerase I, and the 5' cap confers stability to mRNA and affords the ability of mRNA to be translated efficiently in vivo.

Alcohol Oxidoreductases↗
PubMed1997

GTP hydrolysis controls stringent selection of the AUG start codon during translation initiation in Saccharomyces cerevisiae.

We have isolated and characterized two suppressor genes, SUI4 and SUI5, that can initiate translation in the absence of an AUG start codon at the HIS4 locus in Saccharomyces cerevisiae. Both suppressor genes are dominant in diploid cells and lethal in haploid cells. The SUI4 suppressor gene is identical to the GCD11 gene, which encodes the gamma subunit of the eIF-2 complex and contains a mutation in the G2 motif, one of the four signature motifs that characterizes this subunit to be a G-protein. The SUI5 suppressor gene is identical to the TIF5 gene that encodes eIF-5, a translation initiation factor known to stimulate the hydrolysis of GTP bound to eIF-2 as part of the 43S preinitiation complex. Purified mutant eIF-5 is more active in stimulating GTP hydrolysis in vitro than wild-type eIF-5, suggesting that an alteration of the hydrolysis rate of GTP bound to the 43S preinitiation complex during ribosomal scanning allows translation initiation at a non-AUG codon. Purified mutant eIF-2gamma complex is defective in ternary complex formation and this defect correlates with a higher rate of dissociation from charged initiator-tRNA in the absence of GTP hydrolysis. Biochemical characterization of SUI3 suppressor alleles that encode mutant forms of the beta subunit of eIF-2 revealed that these mutant eIF-2 complexes have a higher intrinsic rate of GTP hydrolysis, which is eIF-5 independent. All of these biochemical defects result in initiation at a UUG codon at the his4 gene in yeast. These studies in light of other analyses indicate that GTP hydrolysis that leads to dissociation of eIF-2 x GDP from the initiator-tRNA in the 43S preinitiation complex serves as a checkpoint for a 3-bp codon/anticodon interaction between the AUG start codon and the initiator-tRNA during the ribosomal scanning process.

Amino Acid Sequence↗
PubMed1997

Automatic background recognition and removal (ABRR) in computed radiography images.

A novel method to automatically recognize and remove background signals in computed radiography (CR) images caused by X-ray collimation during projection radiographic examinations is presented. There are three major steps in this method. In the first step, a statistical curve is derived based on many hierarchical CR sample images as a first approximation to loosely separate image and background pixels. Second, signal processing methods, including specific sampling, filtering, and angle recognition, are used to determine edges between image and background pixels. Third, adaptive parameter adjustments and consistent and reliable estimation rules are used to finalize the location of edges and remove the background. In addition, this step also evaluates the reliability of the complete background removal operation. With this novel method implemented in a clinical picture archiving and communication system (PACS) at the University of California at San Francisco, we achieved 99% correct recognition of CR image background, and 91% full background removal without removing any valid image information.

Humans↗
PubMed1997

Full-field direct digital telemammography: technical components, study protocols, and preliminary results.

The early detection of breast cancer increases the survival rate in women. Today, film-screen mammography is the most common and effective technique for the detection of breast cancer. However, the film-screen image recording system of current mammography has several technical limitations that can reduce the breast cancer diagnostic accuracy. A state-of-the-art technology, full-field direct digital mammography (FFDDM) has the potential to increase the sensitivity of clinical and screening examinations and emerge as a film-screen mammography replacement. It is believed that efficiently delivering the superior image quality of FFDDM to expert mammographers will significantly increase the breast cancer diagnostic accuracy. This concept motivates the studies of FFDDM telemammography. In this paper, we will report the FFDDM telemammography project that is being conducted in our facility. The fundamental technology of the FFDDM system and the characteristics of FFDDM images are described. Our research approach is through three protocols: telediagnosis, teleconsultation, and telemanagement. Each of these study protocols is defined. To conduct this FFDDM telemammography project, an asynchronous transfer-mode-network-based telemammography system is developed across two remote campuses in our facility. The architecture of this system is detailed. Unlike other medical imaging modalities, the matrix of an FFDDM image is huge. How to present the image information to mammographers via currently available display media is a challenging task. Our display approaches for the FFDDM images are described. Some preliminary study results from the current phase of this study are reported.

Breast Neoplasms↗
PubMed1996

Clinical experience with a second-generation hospital-integrated picture archiving and communication system.

In a previous report we described a second-generation hospital-integrated picture archiving and communication system (HI-PACS) developed in-house. This HI-PACS had four unique features not found in other PAC systems. In this report, we will share some of our clinical experiences pertaining to these features during the past 12 months. We first describe the usage characteristics of two 2,000-line workstations (WSs), one in the in-patient and the second in the out-patient neuroradiology reading area. These two WSs can access neuro-images from 10 computed tomographic and magnetic resonance scanners located two medical centers through an asynchronous transfer mode network connection. The second unique feature of the system is an intensive care unit (ICU) server, which supports three WSs in the pediatric, medical surgery, and cardiac ICUs. The users' experiences and requests for refinement of the WSs are given. Another feature is physician desk-top access of PACS data. The HI-PACS provides a server connected to more than 100 Macintosh users for direct access of PACS data from their offices. The server's performance and user critiques are described. The last feature is a digital imaging and communication in medicine (DICOM) connection of the HI-PACS to a manufacturer's ultrasound PACS module. The authors then outline the interfacing process and summarize some of the difficulties encountered. Developing an in-house PACS has many advantages but also some drawbacks. Based on experience, the authors have formulated three axioms as a guide for in-house PACS development.

Computer Communication Networks↗
PubMed1996

Design and implementation of a picture archiving and communication system: the second time.

This report describes the authors' experience in the design and implementation of two large scale picture archiving and communication systems (PACS) during the past 10 years. The first system, which is in daily clinical operation was developed at University of California, Los Angeles from 1983 to 1992. The second system, which continues evolving, has been in development at University of California, San Francisco (UCSF) since 1992. The report highlights the differences between the two systems and points out the gradual change in the PACS design concept during the past 10 years from a closed architecture to an open hospital-integrated system. Both systems focus on system reliability and data integrity, with 24-hour on-line service and no loss of images. The major difference between the two systems is that the UCSF PACS infrastructure design is a completely open architecture and the system implementation uses more advanced technologies in computer software, digital communication, system interface, and stable industry standards. Such a PACS can withstand future technology changes without rendering the system obsolete, an essential criterion in any PACS design.

Diagnostic Imaging↗
PubMed1996

Workstation design. Image manipulation, image set handling, and display issues.

The importance of this article is fourfold. First, the introduction of workstation technology and the types of image workstations provides readers with a better understanding of the state-of-the-art and availability of digital image-display workstations in the market-place. Second, this article identifies primary processes related to image viewing in radiology daily operations. This is crucial because it illustrates the important concepts of the Folder Manger with image preprocessing, patient folder organization, and automatic image display sequencing. With these features incorporated in the workstation design, the number of steps required for a radiologist to interact with a workstation is minimized. Third, the discussions on how to present and manipulate images on the workstations suggest methods concerning the issue of displaying large volumes of image sets on a limited number of monitor screens. Lastly, examples of automatic image sequencing, high-resolution color monitors, and voice-based user interface illustrate current research topics in the future of digital workstation design.

Computer Systems↗
PubMed1995

A paradigm for computer-assisted radiology resident rotation scheduling.

RATIONALE AND OBJECTIVES: The scheduling of radiology residents remains a major annual undertaking of chief residents. In this article, we describe a paradigm to implement interactive computer programs to reduce the inefficiencies and inequities of planning the yearly schedule. METHODS: We used the programming language, Prolog, to develop a compact program that provides faster and more flexible performance than those reported in the literature. This interactive program stores scheduling requirements in data files separated from the control program and runs on a Macintosh computer. RESULTS: The schedule of any residency year is generated within 3-7 sec. The fast computation and query capabilities of this scheduling program have helped chief residents to identify conflicting requirements that were previously overlooked. CONCLUSION: Using our programming paradigm, we have developed a portable Prolog-based scheduling program that is quick and easy to use.

Cost-Benefit Analysis↗
PubMed1995

Byssinosis in Guangzhou, China.

OBJECTIVES: To study the prevalence of byssinosis and other respiratory abnormalities in workers exposed to cotton dust in Guangzhou in two factories that processed purely cotton. METHODS: All the 1320 workers exposed were included. The controls were 1306 workers with no history of occupational dust exposure. Total dust and inhalable dust were measured by Chinese total dust sampler and American vertical elutriator respectively. A World Health Organisation questionnaire was used. Forced vital capacity (FVC) and forced expiratory volume in one second (FEV1) were measured by a Vitalograph spirometer. RESULTS: The median inhalable dust concentrations ranged from 0.41 to 1.51 mg/m3 and median total dust concentrations from 3.04 to 12.32 mg/m3. The prevalence of respiratory abnormalities in the cotton workers were (a) typical Monday symptoms 9.0%; (b) FEV1 fall by > or = 5% after a shift 16.8%; (c) FEV1 fall by > or = 10% after a shift 4.2%; (d) FEV1 < 80% predicted 6.1%; (e) FEV1/FVC < 75% 4.0%; (f) cough or phlegm 18.2%; (g) chronic bronchitis 10.9%; and (h) byssinosis, defined by (a) plus (b) 1.7%. With the exception of (d), most of the prevalences increased with increasing age, duration of exposure, and cumulative inhalable dust exposure. No increasing trends of respiratory abnormalities were found for current total dust, inhalable dust, and cumulative total dust concentrations. Compared with controls, after adjustment for sex and smoking, with the exception of (d), all the pooled relative risks of respiratory abnormalities were raised for cotton exposure. CONCLUSION: It is concluded that cumulative inhalable cotton is likely to be the cause of byssinotic symptoms, acute lung function decrements, cough, or phlegm, and chronic bronchitis.

Adolescent↗
PubMed1995

Asynchronous transfer mode technology for radiologic image communication.

Image communication is an important component in picture archiving and communication systems (PACS) and teleradiology applications. Currently, local area networks (LANs) and wide area networks (WANs) use different technologies for image communication. Asynchronous transfer mode (ATM) is an emerging technology that can be used for both LANs and WANs. This article describes experimental results using an ATM network to transmit CT scans and digitized radiographs between the University of California, San Francisco (UCSF) and Mount Zion Hospital, an affiliated community hospital in the San Francisco Bay area. The WAN connection between the two hospitals is via an ATM main switch at Pacific Bell, a local communication carrier located in Oakland, CA, which uses single-mode optical fibers. Preliminary results show that, using the ATM Optical Carrier Level 3 (OC3) (155 Mbits/sec) specification, it takes 1.3 sec and 2.7 sec to transmit a 10-Mbyte digitized radiograph and a 20-Mbyte CT scan, respectively, between the two locations. Encouraged by these results, we have designed and implemented an ATM WAN and LAN between UCSF and Mount Zion Hospital. This is the first of a three-phase project of installing a WAN serving four hospitals and one clinic in the San Francisco Bay area.

Computer Communication Networks↗
PubMed1994

Noise content analysis in clinical digital images.

Most digital modalities in radiology use 12 bits precision to store the digital images. To determine whether all these bits contain useful information, a statistical method called the Moran test was used to measure the noise level in computed tomographic, magnetic resonance, and digital radiographic images. The test was performed on the bit planes of each pixel. After the noise level was estimated, the pixel data were separated into signal bits and noise bits, and image enhancement techniques were applied on the noise-bits-removed images to demonstrate that the removal of noise bits did not affect image quality. Preliminary results showed no noticeable difference between the original images and the noise-bits-removed images.

Algorithms↗
PubMed1994

Digital archive system for radiologic images.

Use of a picture archiving and communication system (PACS) for clinical review requires rapid access to patient images. The authors have developed a second-generation archive system for a departmental PACS that optimizes access time for both current and historical images. The system is based on a composite staging mechanism that uses multiple storage media: magnetic disks, erasable magneto-optical disks, and write-once-read-many (WORM) disks. A two-tiered communication network composed of a standard Ethernet network and a gigabit bandwidth fiberoptic UltraNet network is used for distributing images to remote display stations. The system currently archives 1.5 Gbytes of radiologic images daily from three magnetic resonance imagers, two computed tomographic scanners, one computed radiographic system, and one laser film digitizer. The system features (a) multiple optical drives and robotic arms, which support concurrent archival and retrieval operations; (b) a stacking mechanism, which provides fast retrieval of current images; (c) a pre-fetch mechanism, which minimizes on-line image retrieval, hence relieving peak-hour workload of the optical disk library and the networks; (d) studies grouping and platter management, which optimize retrieval of a patient's images from multiple examinations; and (e) a job-prioritizing control, which minimizes waiting time for radiologists and referring physicians to review images at the display stations. This system is suitable for a large-scale PACS to serve the clinical and research staff.

Radiology Information Systems↗
PubMed1994

Accessing picture archiving and communication system text and image information through personal computers.

Recent advances in storage technology have made possible the archiving of tremendous amounts of text and image information within a picture archiving and communication system (PACS). However, a radiologist's access to this information typically has been limited to viewing workstations designed primarily to support clinical activities. Unfortunately, these workstations often overlook the benefits of PACS in teaching and research applications, which are of significant importance in an academic institution. To support such activities at our own institution, we have included two major objectives in our second-generation PACS development: (1) to provide access to text and image information archived within our PACS in an environment that is easily accessible to and comfortable for our radiologists, namely, their own Macintosh (Apple Computer, Cupertino, CA) personal computers; and (2) to provide this information in standard Macintosh formats, so that tools with which radiologists are already familiar can be used in frequently performed teaching activities--the production of slides and prints, the maintenance of personal teaching and research files, and specialized image analysis and processing.

Computer Communication Networks↗