Biomedical subjects

H Joris

Publications and source records attributed to H Joris.

At least 73 records · Page 4Linked to original sources

PubMed1993

High fertilization and implantation rates after intracytoplasmic sperm injection.

Previously reported better fertilization rate after intracytoplasmic single sperm injection (ICSI) than after subzonal insemination of several spermatozoa was confirmed in a controlled comparison of the two procedures in 11 patients. Intracytoplasmic sperm injection was carried out in 150 consecutive treatment cycles of 150 infertile couples, who had failed to have fertilized oocytes after standard in-vitro fertilization (IVF) procedures or who were not accepted for IVF because not enough motile spermatozoa were present in the ejaculate. A single spermatozoon was injected into the ooplasm of 1409 metaphase II oocytes. Only 117 oocytes (8.3%) were damaged by the procedure and 830 oocytes (64.2% of the successfully injected oocytes) had two distinct pronuclei the morning after the injection procedure. The fertilization rate was not influenced by semen characteristics. After 24 h of further in-vitro culture, 71.2% of these oocytes developed into embryos, which were transferred or cryopreserved. Only 15 patients did not have embryos replaced. Three-quarters of the transfers were triple-embryo transfers. High pregnancy rates were noticed since 67 pregnancies were achieved, of which 53 were clinical, i.e. a total and clinical pregnancy rate of 44.7% and 35.3% per started cycle and 49.6% and 39.2% per embryo transfer. A total of 237 supernumerary embryos were cryopreserved in 71 treatment cycles.

Cytoplasm↗

PubMed1992

Pregnancies after intracytoplasmic injection of single spermatozoon into an oocyte.

Intracytoplasmic sperm injection (ICSI) is a promising assisted-fertilisation technique that may benefit women who have not become pregnant by in-vitro fertilisation (IVF) or subzonal insemination (SUZI) of oocytes. We have used ICSI to treat couples with infertility because of severely impaired sperm characteristics, and in whom IVF and SUZI had failed. Direct injection of a single spermatozoon into the ooplasm was done in 47 metaphase-II oocytes: 38 oocytes remained intact after injection, 31 became fertilised, and 15 embryos were replaced in utero. Four pregnancies occurred after eight treatment cycles--two singleton and one twin pregnancy, and a preclinical abortion. Two healthy boys have been delivered from the singleton pregnancies and a healthy boy and girl from the twin pregnancy.

Adult↗

PubMed1992

Induction of acrosome reaction in human spermatozoa used for subzonal insemination.

Human spermatozoa were injected into the perivitelline space of oocytes from 43 couples (44 cycles) in whom fertilization had failed in conventional in-vitro fertilization (IVF). The spermatozoa were treated to enhance the percentage of acrosome-free spermatozoa either by incubation for 24 h in T6 medium with 50% follicular fluid (v/v) or by incubation for 24 h in T6 medium followed by electroporation and incubation for a few hours in T6 medium with 3.5 mM pentoxifylline. After these two procedures, the mean percentage of acrosome-free spermatozoa increased to 35.5 and 53.9% respectively. Up to three spermatozoa were injected into the perivitelline space of metaphase II oocytes; few oocytes were damaged during the injection procedure. The overall fertilization rate was 30.9% of the 433 oocytes that were intact after subzonal insemination. Only 3% of the injected oocytes had more than two pronuclei. The cleavage rate of the fertilized oocytes was 80%. There was no difference in the fertilization and cleavage rates between the two sperm treatment procedures. One, two or three embryos were replaced in 34 cycles and seven patients became pregnant. In three of the four ongoing pregnancies, prenatal diagnosis by amniocentesis indicated a normal karyotype.

Acrosome↗

PubMed1981

[Corrected transposition of the great vessels in the aged. Presentation of a case, review of the literature on the development and an anatomical and histological study of the conduction system].

A previously healthy 46 year old man was found to have a murmur suggestive of mitral incompetence, when examined after an accident. Investigations revealed corrected transposition of the great arteries and severe regurgitation of the left atrioventricular valve. A complementary bibliographic study of 19 other cases, aged over 40 years old was undertaken to assess the natural history of the condition, apparently fatal before the fifth decade in cases with associated lesions, shunt or valvular disease, but of better prognosis in the rare cases of isolated corrected transposition. The anatomical study suggested a congenital malformation of the regurgitant valve (cleft and abnormal chordal pattern) but a traumatic origin could not be excluded. Examination of the conduction system showed the presence of degenerative lesions. The atrioventricular node was located in the classical "posterior position", which is unusual in this type of congenital malformation.

Aged↗

PubMed1976

[Long-term results in the isolated replacement of mitral and aortic valves].

This is a study of 85 valve replacements by mechanical prostheses, 41 of which were in the aortic position and 44 in the mitral position. The results were analysed from the standpoint of the immediate and late mortality, the morbidity, and thrombo-embolic complications. The influence of the severity factors (NYHA classification) on the operative risk and the late mortality has been evaluated. The maximum follow-up period was 8 years for the aortic replacements and 7 years for the mitral replacements. Actuarial graphs show that there is a 78.3% survival for aortic valve replacement and a 66% survival for mitral valve replacement. We feel that the shape of the survival curves is greatly influenced by the size of population studied. As soon as a plateau appears, the significance of the analysis is in doubt. It seems to us that calculation of the degree of confidence is essential if the good quality of the long-term results is not to be overestimated. The results of surgery must be assessed by comparing the survival curves of the operated patients with those of non-operated patients with the same risk factors. The conclusion emerges that prosthetic replacement of the mitral and aortic valves markedly improves the life expectancy of stage IV patients. We can see no indication for early surgery on the aortic valve. On the other hand, mitral valve replacement should be undertaken on stage III patients so that those with a poor life expectancy can be offered a better outlook. The way in which the mitral and aortic valve disorders carry a much greater mortality after the 6th decade should induce us to bring forward the indications for surgery in this age group in spite of the slightly greater immediate and late risk from operating on patients of this age.

Adolescent↗

PubMed1971