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Biomedical subjects

H Jokela

Publications and source records attributed to H Jokela.

54 records · Page 3Linked to original sources
PubMed1987

Serum selenium, vitamin A, vitamin E and cholesterol concentrations in Finnish and Japanese postmenopausal women.

Serum concentrations of vitamins A and E, selenium, and cholesterol were measured in 30 Finnish and 30 Japanese postmenopausal women. While both groups had identical serum vitamin A levels, the other serum factors differed significantly between the groups, the Finns having higher serum vitamin E and cholesterol and lower serum selenium levels. Adjusted to serum cholesterol, which correlated significantly with vitamin E in both groups, the vitamin E levels were, however, similar in the groups. The differences in the serum levels of these nutritional factors may be associated with the differences in the risk of coronary heart disease and cancer between Finnish and Japanese populations.

Cholesterol↗
PubMed1986

Evaluation of a new inhibitor test for isoamylase on Hitachi 705 analyser.

We describe a simple and rapid method for measuring pancreatic and salivary type amylases using the Hitachi 705 analyser. The determination is based on the inhibition of salivary amylase using an inhibitor isolated from wheat germ. The precision of the proposed method was very good: within-day precision varied from 0.4 to 2.5% (CV) and day-to-day precision from 2.2 to 3.7% (CV). The new application correlated well with another commercially available inhibition method. As the standardization is very stable and the assay procedure exactly the same as for the total amylase assay, the proposed method is suitable for routine isoamylase determination. Reference values for pancreatic and salivary amylase activities are presented.

Autoanalysis↗
PubMed1985

Serum albumin assay with bromcresol purple dye.

Serum albumin was assayed with bromcresol purple using routine clinical chemical photometric analysers. Bromcresol purple and bromcresol green dyes were compared, and albumin assays using these dyes were compared to those made by radial immunodiffusion and rocket immunoelectrophoresis. Bromcresol purple seems to be insensitive to all serum proteins except albumin, and this dye is correlated linearly with radial immunodiffusion and with rocket electrophoresis.

Bromcresol Green↗
PubMed1984

Serum amylase and isoamylase assay on the Hitachi 705 automatic clinical chemical analyzer.

The automated continuous alpha-amylase assay using p-nitrophenyl-alpha-D-maltoheptaoside (Boehringer Mannheim) as substrate on the Hitachi 705 clinical chemical analyzer, was compared with the Phadebas Kinetic Amylase Assay (Pharmacia Diagnostics) on the Hitachi 705. The two methods showed good correlation. The precision varied from 1.0 to 2.5% (CV) within-day and from 1.1 to 5.6% (CV) day-to-day. The substrate, p-nitrophenyl-alpha-D-maltoheptaoside, was also applied to an automated isoamylase assay. The amylase inhibitor from wheat was used to determine the ratio of pancreatic and salivary amylase activities of serum. About 80% of salivary type amylase was inhibited up to an activity level of 1000 U/l, while inhibition of pancreatic type amylase activity was only 10-15%. Ratios of pancreatic to salivary amylase from 0.1 to 10 can be evaluated in serum with wheat inhibitor. The precision of the isoamylase determination by the Boehringer amylase method was acceptable.

Amylases↗
PubMed1984

Quality control of insulin radioreceptor assay for human erythrocytes. Effect of ageing of mono-125I-Tyr-A14-insulin preparation.

The quality control of insulin radioreceptor assay for human erythrocytes is based on the storage of erythrocyte preparations in Hepes buffer of pH 8.0, containing 10 g/l of albumin and 20 mmol/l of glucose. The change of erythrocytes into spherocytes and crenated cells reduces the apparent number of insulin receptors in a relatively constant way by less than 8% a week after 10 days of storage. At the same time the dissociation constants of the insulin-receptor complex increase rapidly. Thus the use of a preparation must be limited to controlling the determination of the insulin binding sites of erythrocytes, and not to the measurement of the affinities of the receptors. When mono-125I-Tyr-A14-insulin gets old, a slow decrease in the insulin binding sites can be measured, but the dissociation constants of the insulin receptor complex are not affected.

Erythrocytes↗
PubMed1983

Fibrinogen: a comparison of an immunoturbidimetric method with four conventional methods.

A rapid and simple immunochemical method for fibrinogen determination is described. The method is based on measuring the turbidity caused by antigen-antibody complexes with an enzyme analyser using diluted antiserum as a starting reagent. This immunoturbidimetric method correlates well with the immunodiffusion method and with two of the thrombin methods. Its correlation with the precipitation method according to Low was a little weaker.

Fibrinogen↗
PubMed1981

Effect of oestriol succinate on serum lipids.

The series comprises 70 women with climacteric symptoms; 14 of them were oophorectomized. The first group of patients received 4 mg of oestriol succinate per day or a placebo in a double-blind experiment. In the second part of the study oophorectomized women were given 8 mg of oestriol succinate per day or 16 mg/day divided into two doses. Serum total cholesterol, HDL-cholesterol and serum triglycerides were estimated before drug treatment and after drug administration for three and six months. Administration of 4-16 mg/day of oestriol succinate was without effect on serum total cholesterol. A significant increase of 25-30% in HDL-cholesterol was observed following administration of oestriol succinate (8 mg divided into two doses) with a rising tendency in total triglyceride value.

Adult↗
PubMed1981

Immunoassay for transferrin using LKB 8600 Reaction Rate Analyzer.

A rapid immunochemical method for transferrin determination is described. The method is based on measuring turbidity caused by antigen-antibody complexes with an enzyme analyser using diluted antiserum as a starting reagent. This immunoturbidimetric method correlates well with TIBC (r=0.979; n=36). A comparison with the radial immunodiffusion method gave the correlation coefficient 0.984 and the regression line y=1.20x-0.54 (n=36).

Antigen-Antibody Complex↗
PubMed1980

Influence of the pollen season on immediate skin test reactivity to common allergens.

We studied the reproducibility of skin prick test reactions and tested 65 young men, picked from a population survey, in spring and fall. We used 16 extracts from two manufacturers with 12 common allergens. The extracts employed on both occasions were from the same allergen batches and the testing procedure was the same. Four subjects who reacted negatively in spring turned positive when re-tested in fall; the opposite happened to one subject. The result remained positive or negative on both occasions in 92%. The best reproducibility (96%) was obtained by pollens. The reactivity against tree and grass pollens was higher in fall than in spring; this is obviously a result of the immunologic stimuli induced by exposure to pollens during the season. The reactions caused by animal danders and house dust were, however, smaller in fall than in spring. This is believed to be due to young people being less exposed to indoor dust in summer. The immediate skin test reactivity is influenced by several factors; we would like to add one: the time of year when the test is applied. The reproducibility of skin prick test results is quite satifactory when the problem is approached as presented here.

Adolescent↗
PubMed1979

Asthma and allergy in Finnish conscripts.

We studied the occurrence of asthma, bronchial wheezing, allergic rhinitis and atopic dermatitis in 295 young men aged 18-19 years. The relationship of these symptoms to the immediate skin test reactivity was also determined. Symptoms indicating past or current allergy and bronchial wheezing were observed in 36%. The cumulative prevalence of asthma was 2.7%, bronchial wheezing 9% in addition, allergic rhinitis (including allergic conjunctivitis) 20%, and atopic dermatitis (including allergic urticaria) 20%. Positive immediate skin prick test reactions were observed in 50% of the population. Allergic rhinitis was most clearly connected with a positive skin test. This study shows that the respiratory disorders, generally considered to be allergic in origin, and atopic dermatitis are more common in Finland than has been assumed. The results are, however, in accordance with the observations made in other industrialized countries. Susceptibility to asthmatic reactions and allergic symptoms should be taken into account, more so than at present, when mudging the capability of a young man to manage compulsory military service.

Adolescent↗
PubMed1978

Enzymatic determination of triglycerides with a system Olli 3000 analyser.

We describe a totally enzymatic method for determining serum triglycerides adapted for a System Olli 3000 analyser. In this procedure triglycerides are determined by measuring free glycerol enzymatically after hydrolysis of a sample with lipase and esterase. The method utilizes two standards for calibration, includes a blank correction and requires 20 microliter of serum. Forty serum samples can be analysed in about 30 min. The procedure is linear up to a concentration of 8 mmol/l of triglycerides. The precision and sensitivity of the method are good. A comparison of this method with another enzymatic method gave the correlation coefficient 0.988 and the regression line y = 1.02x + 0.02 (n = 95).

Autoanalysis↗
PubMed1978

Purification of acid proteinases from calf brain.

The purification was begun with acetone precipitation of minced brain tissue with subsequent proteinase extraction with 0.2 M sodium formate buffer (pH 3.5), reprecipitation with acetone and dialysis. Chromatographic separation on Sephadex G-200, DEAE-Sephadex A-50 and CM-cellulose was carried out in that order. Upon ion-exchange chromatography multiple forms of acid proteinases emerged; two of them were obviously identical with cathepsin D (EC 3.4.23.5), and two of them exhibited properties of cathepsin B (EC 3.4.22.1).

Animals↗
PubMed1977

A discrete multichanneal procedure for the chemical determination of urinary estrogens during pregnancy.

A colorimetric method, based on the Kober reaction, was adapted for the determination of urinary estrogens during pregnancy. The method yields final volumes for direct measurements by a multichannel computer-controlled photometer. The speed of the analysis was increased so that 100 samples can easily be completed by one laboratory technician as triplicate analyses during one working day. A satisfactory accuracy and precision of the method were also achieved. The values were determined using urine samples from 1097 apparently healthy pregnant women.

Colorimetry↗
PubMedDate not supplied

Association between M/L55-polymorphism of paraoxonase enzyme and oxidative DNA damage in patients with type 2 diabetes mellitus and in control subjects.

The paraoxonase enzyme (PON) gene polymorphism causes a change of methionine (M-allele) to leucine (L-allele). PON may reduce low density lipoprotein oxidation and prevent atherosclerosis. Urinary 8-hydroxy-2'-deoxyguanosine (8-OHdG) is a sensitive index of oxidative DNA damage. We have studied the association between the PON genotypes and the urinary excretion of 8-OHdG. The study population consisted of 93 Finnish type 2 diabetes patients and 106 non-diabetic control subjects. The 24-h excretion of 8-OHdG was significantly higher in diabetic patients than in control subjects (P < 0.001). In control subjects, the ratio of the 8-OHdG/glomerular filtration rate increased in order of genotype from MM to ML to LL (P < 0.0412). These results suggest that lipid peroxidation may have an effect on DNA oxidation.

8-Hydroxy-2'-Deoxyguanosine↗