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Biomedical subjects

H Jaksche

Publications and source records attributed to H Jaksche.

42 records · Page 3Linked to original sources

Traumatic, spontaneous and postoperative CSF rhinorrhea.

CSF fistulas are a major complication of head injury but also occur spontaneously or symptomatically in connection with tumours of the skull base, empty sella syndrome, ethmoidal encephalomyelocele, intracranial hypertension or postoperatively in connection with operations on skull base tumours or ENT operations. Their main risk is the possibility of meningitis. The main clinical symptom is CSF leakage from the nose, but meningitis may be the first manifestation. Isotope cisternography and metrizamide CT cisternography are the most important methods for precise localization, sometimes also for verification of a suspected fistula. Most traumatic CSF fistulas of the frontal and ethmoidal region have to be treated operatively. The method of choice is the transfrontal approach and the closure of the fistula opening using a pedicled pericranial flap or fascia lata graft. Most sphenoidal fistulas have to be treated by packing the sphenoidal sinus with muscle. The treatment methods of the rare spontaneous and symptomatic CSF fistulas are also described. The results of operative treatment are satisfactory. About 6% recurrences, which as a rule can be cured by reoperation, and a mortality rate of about 1-3% seem to be an acceptable price for prevention of an otherwise unavoidable and oftenly deadly meningitis. Future efforts are necessary to improve the operative technique in order to reduce the incidence of anosmia. Our descriptions and advice are based not only on literature reports but also on our own experiences with a combined material of 237 cases operated on for rhinorrhea.

Adult↗

Spinal elastance measurement in patients with spondylogenic narrowing of the spinal canal.

Spinal elastance and the time course of pressure decrease after saline injection into the lumbar subarachnoid space was measured in a control group and in a group of patients with spondylogenic narrowing of the cervical spinal canal. The elastance is higher with retroflexion of the head than with anteflexion. This is more pronounced in degenerative diseases of the spine, proving the existence of a pincers mechanism. High elastance values at high volume changes indicate a narrowing of the cervical spinal canal. With a normal spinal canal the pressure decrease shows a slow monoexponential slope (T/2 = 100.8 +/- 13.4 seconds). In patients with partial obstruction of the spinal canal, especially with retroflexion of the head, a secondary pressure increase after termination of the volume injection was observed. This secondary pressure increase decreases in a biexponential manner with a fast slope (T/2 = 37.62 +/- 15.88 seconds) followed by a slow slope. With severe obstructions a plateau remained. This easily performed and well-tolerated measurement can be of aid in the quantification of obstructions of the spinal subarachnoid space.

Cervical Vertebrae↗

Human migration inhibitory factor: purification and immunochemical characterization.

Using gel filtration and preparative isotachophoresis, the migration inhibitory factor (MIF) was highly purified from human lymphocytes activated with concanavalin A. MIF is an acidic protein with a mol wt of approximately equal to 25,000 daltons as determined by gel filtration and analytical polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate. The protein inhibits migration of macrophages in the capillary test and in addition, has a slowing effect on the electrophoretic mobility of guinea pig peritoneal macrophages. Rabbit antibodies specific for this protein, as determined by immunochemical techniques, neutralized the biological effect of MIF on migration and on the electrophoretic mobility of macrophages.

Antigen-Antibody Reactions↗

Escherichia coli enterotoxin: purification, partial characterization, and immunological observations.

Enterotoxin, a diarrhea-inducing protein elaborated by pathogenic Escherichia coli strains, was isolated from the supernate of fermenter cultures of E. coli strain P263, a porcine enteropathogen. Purification involved chromatography and preparative isotachophoresis. The resulting product appeared to be pure according to immunoelectrophoretic, disc electrophoretic, ultracentrifugal, and immunologic criteria. The enterotoxin had an apparent molecular weight of 102,000 daltons, and its isoelectric point was 6.90. The isolated product was active in inducing experimental diarrhea in adult rabbits and piglets. In small dosage it also elicited a drastic increase in adenylate cyclase activity in broken-cell preparations of cat heart tissue. The enterotoxin activity was acid labile and was destroyed by heat (65 C for 30 min). It is suggested that the heat-stable enterotoxin was derived from heat-labile enterotoxin by complexing with endotoxin or with capsular material in the culture supernatant. The antigenic relations between the heat-labile enterotoxins of enteropathogenic E. coli strains of different serological types and different host adaptations, as well as between the E. coli enterotoxin and that of Vibrio cholerae, were investigated.

Animals↗

[Surgical treatment of fronto-maxillary tumors].

Surgical treatment of fronto-maxillary tumors requires interdisciplinary cooperation of maxillofacial surgeons and neurosurgeons. Depending on the site and extension of the tumor, it may be necessary to resort to generous excision of the frontal, temporal, sphenoid, zygomatic bones, orbita and nasal bone, including their soft-tissue covers; possibly even exenteration of the orbita and excision of dura are required. Closure of open subarachnoid space is mandatory. Dural defects are supplied with periosteum or free fascial grafts. Calvarial defect are usually repaired with large rotation flaps of the scalp. In exceptional cases pedicled flaps from more remote areas or microsurgically vascularized grafts are used. The facial area is supplied either with flaps or split skin grafts, depending on the situation. Open paranasal sinuses must be drained via the nasal sinus. Remaining facial defects are treated prosthetically.

Adult↗