Biomedical subjects

H Jagalska-Majewska

Publications and source records attributed to H Jagalska-Majewska.

6 recordsLinked to original sources

PubMed2003

Postnatal development of the basolateral complex of rabbit amygdala: a stereological and histochemical study.

The aim of the study was to estimate developmental changes in the rabbit basolateral complex (BLC) by stereological and histochemical methods. Material consisted of 45 brains of New Zealand rabbits (aged from 2 to 180 days, P2 to P180) of both sexes, divided into nine groups. The following parameters were estimated: volume of the cerebral hemisphere; volume of the whole BLC and of particular BLC nuclei; neuronal density and total number of neurons in these nuclei. Developmental changes in acetylcholinesterase (AChE) activity in the BLC were also examined. The volume of the cerebral hemisphere increased until P30, whereas volumes of nuclei increased for longer--until P90. The density of neurons in all nuclei studied reached the level characteristic for an adult animal at about P30. The total number of neurons in the dorsolateral division of the lateral nucleus (Ldl) stabilized the earliest--between P30 and P60, whereas in the ventromedial division of the lateral nucleus (Lvm), basomedial (BM) and basolateral (BL) nuclei the number stabilized later--between P60 and P90. AChE activity appears minimal in the BLC on P2, reaches a maximum on P30 and then decreases to the level characteristic of an adult animal on P60. AChE activity was greater in BL than in other nuclei in all age groups. Reaching adult AChE activity 1 month earlier than the total number of neurons in the BLC may indicate a role of the cholinergic system in BLC maturation.

Acetylcholinesterase↗

PubMed2001

The amygdaloid complex of the rabbit--morphological and histochemical study.

The aim of the present paper is to describe the morphology and topography of the nuclei of the amygdaloid complex in the rabbit. In the current study we also investigated the intensity of the enzymatic reaction for acetylcholinesterase (AChE) in the amygdaloid complex and the morphology of its neurones. Material consisted of 5 brains of adult New Zealand rabbit, stained either with cresyl violet or for AChE activity. Although, as in other mammals, the rabbit amygdala consists of two main nuclear groups (corticomedial and basolateral), it reveals a peculiar morphology pattern, forming a transition structure between those observed in the cat and rat. Especially characteristic is the arrangement of the basolateral complex. Within that the ventromedial division of the lateral nucleus seems to be the largest, while its dorsolateral division--the smallest. The arrangement of the corticomedial complex in the rabbit is similar to both the cat and rat. In the rabbit the highest acetylcholinesterase activity is found in the basolateral nucleus and the nucleus of the lateral olfactory tract. The lowest AChE staining is observed in the cortical and medial nuclei, amygdalohippocampal and anterior amygdaloid areas and intercalated masses.

Acetylcholinesterase↗

PubMed2000

The connections of the endopiriform nucleus with the insular claustrum in the rat and rabbit.

The connections between two parts of the claustrum in the rat and rabbit were studied using the highly fluorescent lipophilic carbocyanine dye (Dil). After the application of Dil crystal into the endopiriform nucleus, labeled fibers in the insular claustrum were observed in its part directly neighboring the insular cortex and capsula externa. Additionally, numerous projections into the piriform, insular and entorhinal cortices were present. The presence of connections between the endopiriform nucleus and insular claustrum suggests its role concerned with the processes taking part in the allocortical regions as well as in the limbic system.

Animals↗

PubMed2000

Immunohistochemical study of microglial and astroglial cells during postnatal development of rat striatum.

The present study investigates the development of microglial and astroglial cells in the postnatal rat striatum, using immunohistochemical methods with panel antibodies that recognize macrophage antigens of unknown function--ED 1, complement type 3 receptor--OX-42 (for microglia) and glial fibrillary acidic protein (for astrocytes). On the day of birth, ED1/OX-42- immunoreactive microglial cells present in the striatum represent ameboid microglia. Between P0 and P10 we could observe the migration of ameboid microglial cells from neuroepithelial ventricular zone through internal and external capsules into the striatum. During the second postnatal week (P10, P14) a considerable decline of ameboid ED1-immunoreactive microglial cells and an increase of the number of OX-42 positive ramified cells was observed. At P21 only OX-42 positive ramified cells were observed in the whole striatum. On the day of birth, only a few GFAP-positive cells resembling radial glia were observed in the striatum. During the first postnatal week, the number of GFAP-positive cells increased significantly; they showed typical morphology of the astrocytes present in the adult animals. After P21 the final striatal population of astroglia was formed.

Aging↗

PubMed2000

Developmental changes of MAP2 immunoreactivity in the hippocampus proper and dentate gyrus of the rat.

Microtubules are present in high concentration in the nervous system and are a prominent component of the neuronal cytoskeleton. Microtubules are composed of tubulin and variety of microtubule-associated proteins (MAPs), which have been implicated in the regulation of microtubule assembly and function. MAP2 is the most abundant of these proteins, and it has been extensively characterized in various functional and pathological conditions. In the present study the distribution of MAP2 was examined in each layer of the CA1 and CA3 regions of the hippocampus proper and dentate gyrus in rat development. A total of 40 brains at various ages starting from postnatal day (P) 0 to P90 were examined. After perfusional fixation the brains were frozen and cut on the coronal plane and stained with either cresyl violet or standard immunohistochemical methods using the anti-MAP2 antibody. MAP2 exhibited a somatodendritic pattern of localization in cells of the hippocampus. Staining was most prominent in dendrites and perikarya as well as granules surrounding cell bodies. In a newborn rat's brain immunostaining was intense in granules and faint in perikarya. Between P4 and P21 immunostaining density for MAP2 was stronger and appeared in perikarya, granules, and dendritic trees. After P21 the perikarya and dendrites of the pyramidal layer and stratum radiatum of the hippocampus proper, as well as the molecular and granular layer of dentate gyrus, showed reduced immunoreactivity. In the stratum oriens of the hippocampus and polymorphic layer of the dentate gyrus immunoreactivity was still strong until P90.

Age Factors↗

PubMed1999

The basolateral amygdaloid complex--its development, morphology and functions.

For many years the amygdaloid body has been an object of numerous investigations on different species, because the basolateral complex, being the main part of the amygdaloid body, is regarded as "sensory input" to this structure. It plays a very important role in so called emotional memory and learning, what is particularly important in early developmental stages. Impairment at this time may cause psychiatric problems in later life, like neurosis, phobia, unconscious fear or panic attacks. Complicated functions of the basolateral complex require precise control and modulation especially in early development. In this review the morphological changes during the development and maturation will be discussed and compared with neurotransmitter as well as with the expression of the calcium binding proteins at various stages of the development.

Amygdala↗