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H J Malling

Publications and source records attributed to H J Malling.

96 records · Page 6Linked to original sources
PubMed1982

The allergy pricker. Qualitative aspects of skin prick testing using a precision needle.

Skin prick test has advantages over other diagnostic tests in allergy, and attempts to increase the reproducibility are warranted. A standardised disposable precision needle with a needle point of 1.0 mm has recently become available. Based on 960 tests with histamine and grass pollen (Phleum pratense) the reproducibility of the skin prick weal area was calculated. Using histamine 10 mg/ml and grass pollen (1000 and 5000 PNU) a significantly lower coefficient of variation was found compared with a standard blood lancet with a point of 4 mm. The mean weal reaction is reduced to about 80% of the size obtained with the blood lancet. Testing with the precision needle resulted in a significantly reduced incidence of bleedings. The precision needle simplifies the skin test and does not require as much skill as other needles, and is recommended for both routine tests and the biological standardization of allergen extracts.

Adult↗
PubMed1982

Is is possible to predict the clinical effect of hyposensitization?

Sera from 73 grass pollen allergic patients in two double blind studies on hyposensitization were investigated for IgE response towards one major timothy allergen-antigen 19 (Ag 19) by means of crossed radioimmunoelectrophoresis (CRIE). The clinical efficacy of hyposensitization after initial preseasonal treatment was positively correlated with increase in specific IgE binding to Ag 19. It was possible to predict the clinical efficacy by comparing CRIE performed on sera before and after 3 months of treatment.

Allergens↗
PubMed1979

Specific diagnosis of exogenous bronchial asthma in adults. Comparison between case history, intracutaneous test, RAST, histamine release from basophil leucocytes, and bronchial provocation.

Twenty-two adults with extrinsic bronchial asthma were examined with 26 different allergens from five groups: house dust + mite (30 BP), animal dandruff (30 BP), pollen (12 BP), mould fungi (11 BP), and feather + cotton (10 BP). The same allergen batch was used for each allergen extract in all analyses. Based on the results of the single analyses: case history (CH), intracutaneous test (ST), RAST, histamine release from basophil leucocytes (HR), and the results of the analysis combinations and the "true diagnosis" bronchial provocation (BP), the sensitivity and specificity (SE and SP) were calculated and shown graphically. An evaluation of the diagnostic reliability of the single analyses and analysis combination was then possible. Generally, HR was the best individual analysis with very few false positive values and false negative values, followed by RAST, ST, and CH. None of them was able to predict allergy for feather + cotton. Otherwise, BP was positive when HR and rast reached scores 2 and 3 with the exception of RAST for animal dandruff. As regards ST, we had to reach score 3 in order to predict positive BP. CH would only provide the diagnosis with a maximum score in the pollen group. BP was positive in any analysis combination resulting in score 6 or more. ST appeared to be the most sensitive of the individual analyses. All the patients with ST score 0 showed negative BP for all allergen groups, whereas a corresponding sensitivity for HR score 0 was only achieved with animal dandruff, and for RAST score 0 for pollen only. In spite of the new analyses, BP is in many cases necessary if a definite diagnosis is required.

Adolescent↗
PubMed1977

Quantitative and qualitative estimations of IgE bound to basophil leukocytes from hay fever patients.

IgE was removed from human basophils of 4 nonatopic persons and 10 hay fever patients allergic to timothy grass pollen by treating the cells with buffer adjusted to pH 4. IgE could be removed and refixed to the same cells. Refixation was demonstrated by immunofluorescence and by the ability of basophils to release histamine on exposure to timothy pollen. Removed total IgE and specific IgE directed against timothy pollen were estimated, and a linear correlation to the level of total IgE and specific IgE in serum was found. The total number of IgE molecules per basophil was calculated to be in the range of 30,000 to 300,000, and timothy-specific IgE constituted 4%-15% of the total IgE molecules on the cells. It was furthermore established that specific cell-bound IgE was linearly correlated to the pollen concentration releasing 20% of the histamine contents of the basophils. Separated 2gE from sensitized and nonsensitized basophils could be bound to basophils from other patients, resulting in a change in cell sensitivity. This could be ascribed to additional binding to free cell receptors as well as to a partial replacement of bound IgE. Basophils from non-atopic persons could not be sensitized by incubation with surface IgE from atopic persons. The results indicate that acid treatment is a simple method suitable for removing IgE from basophils. This IgE is intact and can be quantitated.

Adult↗