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H J Cho

Publications and source records attributed to H J Cho.

119 records · Page 7Linked to original sources
PubMed1973

Pathogenesis of aleutian disease of mink: nature of the antiglobulin reaction and elution of antibody from erythrocytes and glomeruli of infected mink.

Erythrocytes from mink chronically infected with Aleutian disease virus (ADV) gave positive antiglobulin reactions with rabbit anti-mink immunoglobulin (Ig)G, anti-mink C3, and anti-mink serum, but did not react with anti-mink IgM. The strongest reaction was observed with anti-mink C3. Immunoelectrophoresis demonstrated that serum from rabbits injected with erythrocytes from ADV-infected mink gave a precipitin line with normal mink serum in the beta globulin region corresponding to C3. When normal mink erythrocytes were exposed to serum from ADV-infected mink, they were not sensitized, demonstrating that the antibodies in these mink sera were not directed against erythrocyte antigens. Glycine-hydrochloride buffer treatment of erythrocyte stromata and isolated glomeruli from ADV-infected mink yielded eluates containing serum proteins in the gamma globulin region which appeared to be IgG, and in the beta and alpha globulin regions which are probably complement components. In both erythrocyte and glomerular eluates, anti-ADV antibody was demonstrated. These findings suggested that the positive direct antiglobulin test and glomerulonephritis in Aleutian disease is due to the persistence of ADV and formation and deposition of ADV antigen-antibody-complement complexes on the erythrocyte surfaces and in glomerular capillaries.

Aleutian Mink Disease↗
PubMed1973

Antigen and antibody in Aleutian disease in mink. II. The reaction of antibody with the Aleutian disease agent using immunodiffusion and immunoelectroosmophoresis.

Aleutian disease viral (ADV) antigen was prepared by fluorocarbon extraction of spleen, liver, and lymph nodes from mink experimentally infected ten days previously. Using a potent ADV antigen, antibody was detected by immunodiffusion (ID) and immunoelectroosmophoresis (IEOP). Utilizing these precipitin tests, antibody was detected in all the mink sera tested as early as seven days after experimental infection. Titer of antibody increased throughout the infection period. Titers of more than 100 were reached by 15 days post infection, titers of 1,000 at one month, and titers of more than 5,000 to 10,000 were achieved at two months post infection and thereafter. The immunodiffusion test gave similar or slightly lower titers than those detected by the IEOP. The IEOP test promises to be a most useful technique for the diagnosis of aleutian disease because it is simple, rapid and specific and is capable of detecting infection early in the course of the disease. It is suggested that this test should be utilized especially for the screening of animals purchased or imported as breeding stock onto ranches.

Aleutian Mink Disease↗
PubMedDate not supplied

Prescription behaviours of office-based doctors to standardized common cold patients in Korea.

PURPOSE: To investigate real pictures of prescription behaviours of office-based doctors in Korea, especially focusing on the prescription of oral antibiotics and injections. METHODS: Prescription information was collected from 18 standardized patients (SPs) with the symptoms of the common cold who visited doctors' office. RESULTS: For these patients antibiotics were prescribed by 96 doctors (64.7%) out of 148 and the rate of antibiotic prescription increased with age of doctors, increasing number of medicines, and cost of medicines. Analgesics were most frequently prescribed (91.8%), and gastrointestinal drugs (81.6%), antitussivies (61.2%), antithistamines (61.2%), decongestants (59.2%), mucolytics (51.0%) and proteolytic enzymes (32.7%) followed. More than half of the doctors intended to give injections to the patients, which was higher among internists and doctors prescribed more medicines. CONCLUSIONS: Antibiotics and injections were frequently prescribed for mild common colds in Korea. Action to ensure judicious use of antibiotics and injection is urgent.

Adult↗