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H J Born

Publications and source records attributed to H J Born.

13 recordsLinked to original sources
PubMed1997

[Diagnosis of fetal ovarian cysts. Follow-up or differential diagnosis?].

AIM: To find better diagnostic and prognostic criteria for the prenatal evaluation of suspected fetal ovarian cysts. METHOD: 13 abdominal tumors were diagnosed sonographically and evaluated. The ultrasound equipment was an Acuson Computer Sonograph XP 128 with 3.5 MHz sector transducer (Acuson) and a realtime-ultrasound machine Sonoline SL 1 with 3 MHz linear transducer (Siemens). Picture documentation was made by Agfa Scopix camera, Sony printer documentation UPS or Panasonic video documentation VHS. If a cyst was aspirated, hormonal concentrations were measured by RIA (Radioimmunoassay). Protein levels electrolytes were also analysed and cytology performed. RESULTS: Fetal ovarian cysts are rare connatal abdominal tumours of unknown pathogenesis. Typical sonographic findings are cystic structures, partially septated with homogenous inner structures and sharp outer contours. Their ovarian origin is documented by demonstrating high hormonal activity (estrogen, progestin, testosterone). Sonographic structural variations may hint at tissue trauma or a rare fetal teratoma. CONCLUSIONS: Ultrasound guided cyst puncture with fluid analysis is helpful in the differential diagnose of suspected fetal ovarian cysts. This approach will also help avoid obstetric procedures with possible prematurity complications and post-delivery abdominal surgery. Ultrasound can be used for monitor clinically asymptomatic, uncomplicated cysts, both pre- and postnatally to avoid removal of normal ovarian tissue.

Biopsy, Needle↗
PubMed1993

[Diagnosis of rupture of fetal membranes: comparison of vaginal detection of fetal fibronectin and intra-amnion injection of indigo carmine].

Amniotic fluid contains a heavily glycosylated pregnancy-related isoform of fibronectin (fFN), which can be detected using FDC-6, a monoclonal antibody directed against a novel epitope specific to fFN. It has been identified in the amniotic fluid in various concentrations throughout the different pregnancy periods and is localised at the interface of the chorion and the uterine decidua. Because of the unique pregnancy-associated expression of fFN, we determined the utility of cervicovaginal appearance of fFN as a marker of membrane integrity or rupture of membranes. In a prospective study we compared 1) the detection of fFN in cervicovaginal samples by a membrane-immunoassay (ROM-Check, Mast Diagnostica, Reinfeld, FRG) with 2) the results of intraamniotic injection of Indigo Carmine (IC), followed by the observation of dye spill into the vagina to confirm rupture of membranes. 65 patients were examined, 12 patients had to be excluded from the study for various reasons. Examination results were identical in 50 patients, while in three cases the fFN results were positive with a simultaneous negative result of the intraamniotic dye injection. In the case of blood contamination, the fFN test results were not valid; testing of maternal blood and of urine samples was fFN negative in all cases. We conclude, that oncofoetal fibronectin is an excellent marker for rupture of membranes. The test is easy to perform and does not involve the risk of an intraamniotic injection of dye.(ABSTRACT TRUNCATED AT 250 WORDS)

Amnion↗
PubMed1988

Investigations upon the mechanism of inhibition of spermatogenesis in the rat by a dimeric ethynodiol-testosterone ester.

The combination of androgens and progestogens has been shown to be a suitable male contraceptive. Previous experiments revealed that injection of a dimeric testosterone-ethynodiol ester into rats and monkeys induces azoospermia for several weeks. In order to investigate the mechanism of action, we compared the endocrine effects of a single injection of 10 mg of the dimeric ester into intact male rats with that of 6 mg of norethisterone enanthate + 6 mg of testosterone enanthate. After the injection of the dimer there was a transitory reduction of serum FSH and a strong suppression of serum LH and testosterone, of testicular testosterone and of androgen-binding protein (ABP) in the testis and epididymis for at least 8 weeks, whereas spermatogenesis was totally depressed between the 4th and 8th week. Contrary to this, the enanthates caused only a slight suppression of spermatogenesis, although serum LH, testicular testosterone and ABP were profoundly reduced. The only conspicuous difference in the endocrine pattern of both groups during the first 4 weeks was in the serum testosterone level which remained normal in the rats treated with the enanthates. The results suggest that testicular testosterone and ABP concentrations are of minor significance for an intact spermatogenesis, and that some other factors produced by Sertoli cells might be involved and possibly maintained by normal serum testosterone levels.

Androgen-Binding Protein↗
PubMed1985

Dose- and time-dependent effects of a dimeric ethynodiol-testosterone depot-preparation in female rat.

The time- and dose-dependent effects of a dimeric ethynodiol-testosterone ester upon intact and hemi-castrated female rats were compared to those of equivalent doses of norethisterone enanthate plus testosterone enanthate. In intact rats, serum LH was markedly suppressed for at least 8 weeks after a single i.m. injection of 10 or 20 mg of the dimer which exceeded the depot-effect of the combination of the enanthates. A biphasic time-and dose-dependent effect of the dimeric ester upon ovarian and uterine weight was observed. During first 2 weeks following the injection there was a pronounced enlargement of corpora lutea and a marked enhancement of progesterone secretion, probably due to a direct stimulation by the androgens. At the same time, the uteri were markedly enlarged. During the following weeks, the ovaries became progressively smaller and showed degenerative changes, the steroid levels decreased and the uteri became atrophic. When hemi-castrated rats were injected once with 1 and 5 mg of the dimer, LH became suppressed, and the compensatory hypertrophy of the ovary was inhibited. At higher doses, the weight of the ovary and uterus was increased, and the luteal function was stimulated. The results indicate that, even though the gonadotropins are suppressed, there is possibly a direct stimulatory effect of high doses of the intact dimeric molecule upon the uterus, while the stimulation of the corpus luteum function is due to an interference of the transiently elevated testosterone level with ovarian steroid biosynthesis.

Animals↗
PubMed1984

[Multiple image artifacts with linear sonic transducers].

The multielement arrays used nowadays for diagnostic purposes can, by means of digital signal processing, produce exact images with no problem. When physical limits were reached however, geometric image artifacts also became more evident, thereby making diagnosis more difficult or uncertain. Multiple images became especially evident during tests in the early stages of pregnancy, making it harder to differentiate between single and multiple pregnancies , particularly as dynamic processes such as the heart beat can be shown double at a certain phase in the pregnancy. Artifacts were also observed in the form of multiple echos during control checks on the position of intrauterine contraceptive devices and when investigating gynaecological tumors. These artifacts are ascribed to sonic behaviour at interfaces and are representable as a function of the sonic angle of incidence primarily in one plane--the cross section. They appear virtually symmetrical and can be wiped out by moving the sonic transducer. Although there is no problem in recognising the double phenomena as being artifacts in checks on the position of IUD's, all multiple structures should be clarified by suitable examination techniques especially during the early stages of pregnancy and with tumours.

Diagnosis, Differential↗
PubMedDate not supplied

Laboratory diagnosis of congenital human cytomegalovirus infection using polymerase chain reaction and shell vial culture.

Congenital HCMV infection was diagnosed at the 22th week of gestation. The infection was suspected because HCMV IgM was detected in a serum sample obtained from the woman's husband. HCMV infection was detected in the amniotic fluid by polymerase chain reaction, shell vial culture (immunoperoxidase assay) and conventional virus isolation. Serologic testing in paired sera of the woman and in umbilical cord blood for specific IgM and IgA remained negative. As serological data (preconceptional HCMV serostatus) were incomplete, a clear differentiation between primary and secondary infection could not be achieved; consequently, risk quantification could not be determined. Viruria was detected in the offspring during the 1st week post partum. No clinical signs of cytomegalic inclusion disease were diagnosed up to six weeks post partum. Our case report indicates that for pregnancy surveillance, serologic testing for HCMV antibody should also be performed in the spouse.

Adult↗
PubMedDate not supplied

[Prenatal chromosome analysis using the FISH technique allows fetal aneuploidy detection within a few hours].

Last years evaluation of fluorescence-in-situ-hybridization (FISH) allowed detection of chromosomal abnormalities by using DNA probes, binding to chromosomes in the nucleus. Because it is possible to directly examine interphase nuclei, FISH-technique, in contrast to traditional cytogenetic analysis has the advantage of small loss of time in case of urgent decisions on perinatal management. Karyotyping was performed on fetal cells, obtained from 72 pregnancies after amniocentesis, by both classical cytogenetics and fluorescence in situ hybridization using commercially available kits which utilise the alpha satellite probes for chromosomes 13 + 21 and 18. The classical cytogenetics demonstrated that the fetal karyotype was normal in 67 cases and abnormal in 5 cases (four with trisomy 21 and one with a translocation trisomy 18). With the FISH-technique it was possible to obtain accurate diagnosis of trisomy 21 within 24 hours of sampling. The distribution of the number of signals in the chromosomally normal and abnormal fetuses was significantly different, but we were not able to identify the fetus with translocation trisomy 18. We conclude that in the investigation of fetuses with ultrasonographic diagnosed malformations, FISH provides a rapid technique for detection of numerical chromosomal aberrations, but replacement of classical cytogenetics is not possible because of its limitations for identification of subtle structural chromosomal abnormalities.

Amniocentesis↗