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Biomedical subjects

H Itakura

Publications and source records attributed to H Itakura.

At least 145 records · Page 8Linked to original sources
PubMed1988

Eosinophilic globules in fibroadipose tissue adjacent to Kaposi's sarcoma.

Eosinophilic globules have been reported in the sarcomatous tissue of the cutaneous type of Kaposi's sarcoma. This report presents a case in which similar eosinophilic globules were found in uninvolved area of fibroadipose tissue adjacent to the sarcomatous tissue in a 30-year old African woman. The histochemical characteristics of these globules were identical to those found within the sarcomatous tissue. The origin of these globules is not known, but a possible explanation is offered.

Adipose Tissue↗
PubMed1987

Cloning and regulation of rat apolipoprotein B mRNA.

Recombinant cDNA clones that code for apolipoprotein B(apoB) were isolated from a rat liver cDNA library, using synthetic oligonucleotide probe derived from the sequence of human apoB cDNA. The nucleotide and deduced amino acid sequences of the rat apoB clone pRB5, 1.2 kb in length, showed 83% and 84% homology to those of human apoB. Northern blot analysis revealed that rat apoB cDNA probe cross-reacts with human and rabbit apoB mRNA sequences and the size of those mRNAs, approximately 15 kb long, were not discernibly different. In addition, apoB mRNA was abundant only in the liver and intestine. Finally, cholesterol feeding to rats for six weeks resulted in a several-fold increase in the level of apoB mRNA in the liver.

Animals↗
PubMed1987

Ultrastructural study of lymphocytic interaction with hepatocytes and endothelial cells in acute non-A, non-B hepatitis.

In the liver biopsy specimens of all six patients with acute non-A, non-B hepatitis, the lymphocytic interaction with hepatocytes and sinusoidal endothelial cells was observed by electron microscopic study. Lymphocytes were in a close contact with damaged hepatocytes and interrupted endothelial cells, and the microvilli on the surface of these damaged hepatocytes were degenerated and lost. These findings pointed out the possibility that the lymphocyte may play one of the important roles in hepatocytic damage and endothelial cell damage in acute non-A, non-B hepatitis.

Acute Disease↗
PubMed1987

A staining method for easy detection of eosinophilic globules in Kaposi's sarcoma.

Eosinophilic globules provide an important clue for diagnosis of Kaposi's sarcoma (KS). These globules were stained with periodic acid Schiff (PAS), periodic acid Schiff reagent after diastase digestion, phosphotungstic acid hematoxylin (PTAH) and autofluorescence under ultraviolet illumination. By these methods, however, eosinophilic globules in KS could not be readily identified. Therefore, we developed a new procedure for rapid identification of eosinophilic globules in KS tissues.

Humans↗
PubMed1987

New substrate for determination of serum lecithin:cholesterol acyltransferase.

Serum lecithin:cholesterol acyltransferase (LCAT) was estimated by enzymatically measuring the decrease in unesterified cholesterol after incubation of serum with liposomes. A high-performance liquid chromatography (HPLC) study showed the uptake of the lipids of liposomes by serum high density lipoprotein. Of all the examined liposomes prepared from cholesterol and various synthetic phosphatidylcholines, liposomes with dimyristoylphosphatidylcholine (DMPC) were found to be the most reactive in the LCAT reaction. When serum was used as an enzyme source, addition of purified apolipoprotein A-I, which is known to be an endogenous activator of LCAT, to the assay mixture resulted in a slight decrease in enzyme activity. Using DMPC-cholesterol liposomes as the substrate, the LCAT activities in 120 human sera showed a mean value of 485.4 +/- 64.6 nmol/hr per ml (mean +/- SD), which is 4.4- to 5.4-fold higher than the values obtained by self-substrate methods. LCAT activity was a linear function of the serum sample volume up to 670 nmol/hr per ml and coefficients of variation (CV) less than 4% were obtained under the standardized conditions. Moreover, when partially purified LCAT was added to various heat-inactivated sera, the activity was efficiently recovered. These results suggest that this method is sensitive, reproducible, and not greatly influenced by serum components.

Cholesterol↗
PubMed1986

Lipid metabolism in endotoxic rats: decrease in hepatic triglyceride lipase activity.

Studies were conducted to investigate the effect of E. coli endotoxin administration on hepatic triglyceride lipase (H-TGL) activity in rats, since H-TGL activity is known to behave differently from lipoprotein lipase (LPL) activity in various situations. Plasma triglyceride and free fatty acid concentrations were markedly elevated in animals after injection of endotoxin. Cholesterol and phospholipids were also increased significantly. Lipoprotein analysis by ultracentrifugation showed that the most pronounced increase of lipoproteins was in the VLDL and IDL fractions. Triglyceride lipase activities in post-heparin plasma were markedly decreased. A selective assay for H-TGL activity using a specific antibody revealed that this enzyme as well as LPL is significantly decreased (26% of control) in endotoxic animals. Thus, the increase of VLDL and IDL appears to result from the decrease of both of LPL and H-TGL.

Animals↗
PubMed1986

Liver morphology in marmosets infected with epidemic non-A, non-B hepatitis in India.

In this study the morphological changes in the livers of marmosets inoculated with stool extracts from epidemic non-A, non-B hepatitis patients in India were examined. The histologic changes of epidemic non-A, non-B hepatitis in marmosets consisted mainly of round cell infiltration in the portal tracts, spotty liver cell necrosis, sinusoidal lymphocyte infiltration, and Kupffer cell mobilization. By electron microscopy, liver cells from infected marmosets showed cisternal dilation of the endoplasmic reticulum, irregularly-shaped nucleus, and disorganization of the mitochondrial cristae. In some areas interaction of lymphocytes with hepatocytes was observed. Similar observations have been made in type B hepatitis infection, presumably due to liver cell damage mediated by immune mechanisms. The result of our study is also compatible with the interpretation that the liver cell damage in this experimental model may be mediated by immune mechanisms.

Animals↗
PubMed1986

Eosinophilic globules in Kaposi's sarcoma. A histochemical, immunohistochemical, and ultrastructural study.

Of the 12 cases, 2 (17%) showed eosinophilic globules in the typical cutaneous type of Kaposi's sarcoma. The globules were stained with periodic acid-Schiff (PAS), periodic acid-Schiff reagent after diastase digestion, and phosphotungstic acid hematoxylin (PTAH), but were not stained with Mayer's mucicarmine, and alcian blue. As the results, these globules might be glycoprotein. The shape of the globules was very similar to glycoprotein globules of yolk sac tumor (endodermal sinus tumor) in the tissue of the ovary and testis. In the yolk sac tumor, similar globules are stained with alpha-fetoprotein, beta-subunit of human chorionic gonadotropin, and alpha-1-antitrypsin using immunohistochemical techniques. Immunoperoxidase investigations were done with antibodies to alpha-fetoprotein, beta-subunit of human chorionic gonadotropin, alpha-1-antitrypsin, and carcinoembryonic antigen in the eosinophilic globules of Kaposi's sarcoma, but these antigens were detected in the globules. The morphogenesis of the glycoprotein globules is not clear yet. A better understanding of the source of globules in Kaposi's sarcoma awaits further research.

Carcinoembryonic Antigen↗
PubMed1985

Statistical analysis of relationship between iron accumulation and hepatitis B surface antigen.

Statistical analysis was carried out to determine the relationships of iron deposition among hepatocytes, Kupffer cells, bile duct epithelial cells, portal tracts, and spleens using 68 autopsy cases obtained from Kenya, which included cases of Bantu siderosis. Of 68 cases, 33 (49%) showed siderosis. Cirrhosis was observed in 19 cases, of which 10 (53%) showed siderosis. However, there was no correlation between iron deposition and cirrhosis (Pearson chi 2 = 0.6734). The finding suggests that iron accumulation does not lead to cellular injury. On the other hand, there was a strong association between iron deposition in the livers and that in the spleens. There was also a strong correlation between the presence of hepatitis B surface antigen and iron deposition in the Kupffer cells and in the spleens. Therefore, it is suggested that the hepatitis B virus infection affects the iron metabolism of the reticuloendothelial system.

Female↗
PubMed1985

Normotriglyceridemic abetalipoproteinemia in infancy: an isolated apolipoprotein B-100 deficiency.

The plasma lipoproteins of a 1-year-old Japanese infant were studied because of malnutrition, severe decrease in plasma lipid level, and acanthocytosis. Plasma lipoprotein analysis revealed that low-density lipoproteins were deficient; however, low levels of triglyceride-rich lipoproteins were found in the plasma. On sodium dodecylsulfate (SDS) polyacrylamide gel electrophoresis, apoprotein B-48 and a faint band corresponding to apoprotein B-100 were detected in the lipoprotein fraction of density less than 1.006 g/mL when the infant was 6 months old. Apoprotein B-48 was more clearly detected after 1 year, but the band corresponding to apoprotein B-100 on the sodium dodecylsulfate gel electrophoresis had disappeared. The apoprotein B-48 content of the fraction with density less than 1.006 g/mL was about 0.05 to 0.3 mg/dL. The patient's lipoproteins consisted mainly of high-density lipoproteins. These results suggest that the disorder in this patient is caused by apoprotein B-100 deficiency.

Abetalipoproteinemia↗
PubMed1983

Abnormalities in plasma lipoprotein in familial partial lecithin:cholesterol acyltransferase deficiency.

Abnormalities in plasma lipoproteins from patients with familial partial lecithin:cholesterol acyltransferase deficiency were studied. In these patients the plasma cholesterol ester ratio was about 40% and plasma apolipoprotein B level remained within the normal range. The content of large-sized low-density-lipoproteins (LDL) was low. Apolipoprotein B-100 and B-48 were detected in very-low-density lipoproteins (VLDL) and LDL in patients' plasma. In patients' LDL, apolipoprotein B-48 was primarily present in large-sized particles. Apolipoprotein E and A-I were mainly detected in intermediate-sized LDL. High-density lipoproteins (HDL) were separated into three fractions by gel permeation chromatography. Large-sized HDL particles (150-200 A) including discoidal particles contained apolipoproteins, E, A-IV and A-I. The content of discoidal HDL was low and, on electron micrograph, rouleau-formed particles were rarely seen. Normal-sized HDL (80-100 A) contained apolipoproteins A-I and A-II and small-sized HDL (about 60 A) contained only apolipoprotein A-I. Although several lipoprotein abnormalities were similar to those in classical familial lecithin:cholesterol acyltransferase deficiency, remaining lecithin:cholesterol acyltransferase activity may, however, cause a lack of reduction of apolipoprotein B level, a low level of large-sized LDL and also a low level of discoidal HDL.

Adult↗
PubMed1983

Analysis of apolipoproteins B100 and B48 by sodium dodecyl sulfate polyacrylamide gradient gel electrophoresis.

Apolipoproteins B100 and B48 in human and rat plasma were studied by using sodium dodecyl sulfate (SDS) polyacrylamide gradient gel electrophoresis. On SDS gradient gel electrophoresis, human and rat apoprotein B100 co-migrated and had an apparent Mr equal 258,000 +/- 12,000. Human and rat apoprotein B48 had an apparent Mr equal 189,000 +/- 6,000. The molecular weight of human apoprotein B100 determined by sedimentation equilibrium analysis was 270,000 +/- 20,000, which was similar to the value determined by SDS gradient gel electrophoresis. However, on SDS polyacrylamide gel electrophoresis at constant concentration, the relative migration value of human apoprotein B100 was not constant when the concentration of polyacrylamide was changed. These results indicate that SDS gradient gel electrophoresis is more suitable for the analysis of apolipoprotein B's than ordinary SDS polyacrylamide gel electrophoresis.

Animals↗