OBJECTIVE: The effects of dietary diacylglycerol (DG) on postprandial lipemia in healthy humans were investigated. METHODS: Forty normolipidemic male volunteers ingested fat emulsions containing either DG oil or triacylglycerol (TG) oil, at different doses: 10 g (n = 13), 20 g (n = 10) and 44 g (n = 17). Two test emulsions were given at seven-days intervals in random order. Fatty acid compositions of the test oils had been adjusted to be equal. Fasting and postprandial serum lipid concentrations in each group and plasma lipoprotein lipids in the 20 g-fat ingestion group were measured during the postprandial intervals. RESULTS: When DG emulsion was ingested, serum TG concentrations were significantly lower (p < 0.05) in the late postprandial phase, i.e., 4 hours, 6 hours as compared to the TG emulsion. The magnitude of postprandial lipemia (the area bounded by the curve above the fasting concentration) after ingestion of 44 g-DG emulsion was significantly less than that of 44 g-TG emulsion (6.54 +/- 5.12 and 8.45 +/- 7.54 mmol x h/L, mean +/- SD, respectively). Chylomicron TG, cholesterol, and phospholipid concentrations at 4 hours after ingestion of DG emulsion were significantly lower (p < 0.05) than those after the ingestion of TG emulsion at the same time point. No marked differences were observed for VLDL, LDL and HDL lipids between the test emulsions. CONCLUSION: In the usual range of fat intake (10-44 g), postprandial response after ingestion of DG emulsion was significantly less than that after ingestion of TG emulsion in healthy human subjects.

Adult↗

PubMedDate not supplied

A clinico-pathological study of 163 untreated cases of chronic hepatitis C.

We performed a clinico-pathological study of 163 untreated cases of chronic hepatitis C. Eighty five percent of the patients were clinically asymptomatic and their physical examinations showed unremarkable or minimal changes at the time of the liver biopsy. Liver function tests tended to present slight abnormalities, involving mild elevations of the activity of the aminotransferases and gamma-glutamil transferase levels. In spite of these mild abnormalities, advanced chronic liver disease was histologically detected in eighty nine percent of the patients, mainly showing chronic active hepatitis. The most characteristic histological finding was an interlobular bile duct damage, which correlated with the presence of lymphoid aggregates in the portal tracts and with the development of fibrosis.

Adult↗

PubMedDate not supplied

Giant cell pneumonia: light microscopy, immunohistochemical, and ultrastructural study of an autopsy case.

An autopsy case of measles giant cell pneumonia with intranuclear inclusion bodies is reported. This case of giant cell pneumonia was studied by light microscopy and immunohistochemistry using monoclonal and polyclonal antibody to measles and by electron microscopy (EM). Light microscopic examination showed multinucleated epithelial giant cells with intranuclear and intracytoplasmic inclusions. The giant cells contained prominent, sharply marginated, eosinophilic intranuclear inclusions typical of classic measles pneumonia. Presence of measles antigen was confirmed using both monoclonal and polyclonal antibodies by peroxidase antiperoxidase method. Monoclonal antibody stained positively for intracytoplasmic and intranuclear inclusions. Electron microscopic examination of lung tissue showed intranuclear inclusions of filamentous or worm like nucleocapsid materials in multinucleated epithelial giant cells. The results suggest that this is a case of measles giant cell pneumonia and the intranuclear inclusion bodies are measles viral particles.

Autopsy↗

PubMedDate not supplied

Long-term trend of decreasing frequency of hepatitis B virus infection.

Statistical and histopathological analysis was carried out to determine whether hepatitis B surface antigen (HBsAg) with the autopsy diagnosis of hepatitis, cirrhosis, and hepatocellular carcinoma declined during 1964-1991. In this study, the liver specimens from 673 autopsy cases at Nagasaki University Hospital were used, and these materials were stained for HBsAg with immunoperoxidase method. For statistical calculation, we used Cochran's chi-square test with one degree of freedom for linear trend for proportions. We investigated HBsAg positive rates for the four time periods, 1964-1970, 1971-1977, 1978-1984, and 1985-1991, and for the diagnosis of hepatitis, cirrhosis, and hepatocellular carcinoma. The decreasing linear trend of HBsAg positivity with time was significant for hepatocellular carcinoma and cirrhosis.

Carcinoma, Hepatocellular↗

PubMedDate not supplied

Bridging language and cultural barriers between physicians and patients.

OBJECTIVE: This study explored a group of primary care physicians' use of various methods to bridge language and cultural barriers between themselves and their patients and the physicians' perceptions of the availability and quality of these methods. METHODS: The authors mailed a questionnaire to 495 primary care physicians in the Greater Bay Area of northern California, an area chosen for its ethnically diverse population. Respondents were asked to estimate how many patients they saw per week, how many encounters they had per week with non-English-speaking patients, and how often they used each of six interpretation methods. They were also asked to assess the availability and quality of interpretation services. RESULTS: Physicians reported that, on average, 21% of visits were with non-English-speaking patients. Trained medical interpreters or the AT&T Language Line were used, on average, in fewer than 6% of these encounters, and no interpreters were used in 11%. In 27% of encounters with non-English-speaking patients, the physician could speak the patient's language, in 20% interpretation was done by a staff member who had no formal interpretation training, and in 36% a family member or companion of the patient interpreted. Physicians who had access to trained interpreters reported a significantly higher quality of patient-physician communication than physicians who used other methods (P < 0.0001). CONCLUSIONS: In an area of great ethnic diversity where physicians who had access to the services of trained interpreters reported a significantly higher quality of patient-physician communication, the low rates of use of trained interpreters suggest that factors other than quality, such as costs, preclude greater use of these services.

Adolescent↗