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Biomedical subjects

H Ishihara

Publications and source records attributed to H Ishihara.

At least 541 records · Page 30Linked to original sources
PubMed1985

The core molecule from type H proteoglycan. Release of mannose-containing oligosaccharides by digestion with N-oligosaccharide glycopeptidase.

Chick-embryo cartilage contains a unique set of proteoglycans. Type H proteoglycan (PG-H) is the most abundant, constituting over 90% of the total cartilage hexuronate. We previously showed that treatment of PG-H with chondroitinase ACII and keratanase yields a protein-enriched core molecule [PG(-CS,KS)] with enzymically modified linkage oligosaccharides of the chondroitin sulphate and keratan sulphate chains. We report here that further treatment of PG(-CS,KS) with pepsin and N-oligosaccharide glycopeptidase (almond glycopeptidase) released four distinct types of mannose-containing oligosaccharide. Two of them were shown to be: (Formula: see text). Of the mannose-containing glycopeptides formed by pepsin digestion, about 40% (as mannose) were resistant to N-oligosaccharide glycopeptidase. Since the resistant fraction was enriched in keratan sulphate remnants, it is suggest that the mannose-containing oligosaccharides in this fraction represent those located in a keratan sulphate-enriched region of PG-H.

Amidohydrolases↗
PubMed1985

Lipid composition of the plasma membrane isolated from hyperplastic nodules of rat liver.

Hyperplastic nodules and hepatomas were induced in livers of rats fed a diet containing 0.05% N-2-fluorenylacetamide (2-FAA). The lipid contents, and phospholipid and fatty acid compositions were analyzed in plasma membranes (PM's) isolated from these tissues and normal rat liver, and the following trends were observed. The molar ratio of cholesterol to phospholipid-phosphorus (phospholipid-P) increased in the order: hepatoma less than normal liver less than hyperplastic nodules. The molar percentage of plasmalogen to phospholipid-P decreased in the order: hepatoma = hyperplastic nodules greater than normal liver. The percentages of choline phosphoglycerides (sum of phosphatidylcholine and lysophosphatidylcholine) and ethanolamine phosphoglycerides (sum of phosphatidylethanolamine and lysophosphatidylethanolamine) both decreased in the order: hepatoma greater than hyperplastic nodules greater than normal liver. On the other hand, the percentages of sphingomyelin and phosphatidylserine both increased in the order: hepatoma less than hyperplastic nodules less than normal liver. As regards fatty acid composition, the percentages of both 18:1 and 18:2 decreased in the order: hepatoma greater than hyperplastic nodules greater than normal liver. Those of 18:0 and 20:4 increased in the order: hepatoma less than hyperplastic nodules less than normal liver. These results suggested that the lipid bilayer in PM of hyperplastic nodules has characteristics roughly intermediate between those of hepatoma and liver PM's, although the molar ratio of cholesterol to phospholipid-P in hyperplastic nodules PM was not intermediate.

Animals↗
PubMed1985

Intraventricular administration of cyclo(His-Pro), a metabolite of thyrotropin-releasing hormone (TRH), decreases water intake in the rat.

We explored the effect of cyclo(His-Pro) on drinking in water-deprived rats. Intraventricular administration of cyclo(His-Pro) significantly reduced water intake in these rats in a dose-dependent fashion (3 X 10(-10) -3 X 10(-8) mole), whereas its intraperitoneal injection did not cause any effect on water intake. The inhibitory potency of cyclo(His-Pro) was found to be similar to that of thyrotropin-releasing hormone (TRH), but cyclo(His-Pro) did not significantly interfere with TRH binding in the rat brain. The data indicate that cyclo(His-Pro) action was not mediated through the TRH counterpart. These results suggest that cyclo(His-Pro) may play a potential role in the regulation of water intake in the rat brain.

Animals↗
PubMed1985

Cloning of a gene from Streptomyces species complementing argG mutations.

A gene that complements argG mutations was cloned from Streptomyces coelicolor and Streptomyces lividans by using pIJ702 as a vector. The recombinant plasmid pMCP25 complemented argG mutations of S. lividans and S. coelicolor. The inserted DNA of pMCP25 was able to hybridize with argG+ strains of S. lividans, S. coelicolor, S. lavendulae and S. alboniger but not with argG mutants of these strains.

Chromosome Deletion↗
PubMed1985

Comparative study of the oligosaccharides of human thyroglobulins obtained from normal subjects and patients with various diseases.

Asparagine-linked oligosaccharides were released quantitatively by N-oligosaccharide glycopeptidase (almond) digestion from human thyroglobulins prepared from thyroid glands of normal subjects and patients with several pathological conditions. The pyridylamino derivatives of the oligosaccharides were prepared and analyzed by high-performance liquid chromatography. The content of high-mannose-type oligosaccharides was comparable to that of the complex type in normal thyroglobulins. Man9GlcNAc2 was the predominant component in the high-mannose-type region, while biantennary oligosaccharides with fucose were the major components in the complex-type region. High-mannose-type oligosaccharides were markedly decreased in thyroglobulins prepared from patients with various disorders, such as Basedow's disease, papillary carcinoma, and adenomatous goiter, whereas they were appreciably increased in thyroglobulin from diffuse goiter.

Adenoma↗
PubMed1985

Criterion for early prediction of coronary artery involvement by clinical manifestations in patients with Kawasaki disease.

One hundred and thirty patients with Kawasaki disease were examined by the clinical symptoms and findings in order to predict the coronary artery involvement in the early stage. These patients were tested by the two-dimensional echocardiography iteratively from the acute stage and categorized into the following three groups: Normal coronary artery, transient dilatation of the coronary artery and aneurysm of the coronary artery persisting eight weeks or more after the onset of the illness. Eighteen clinical symptoms and findings within the 14th, 21st and 35th day of the illness were studied. As a result of analysis, a criterion for the early prediction of the coronary artery involvement by clinical manifestations was established as follows: The patients who satisfy the following or are highly suspectable of having a coronary artery involvement: Fever persisting for 14 days or more. Fever persisting for 10 to 13 days, and when two or more out of the following four conditions are satisfied: The age at the onset of the illness is under one year. The lowest value of hemoglobin is 10.0 g/dl or less. The highest white blood cell count is 14000/cmm or more. The lowest value of serum albumin is 3.5 g/dl or less. Two false negative cases (6.5%) were recognized in 31 of the high risk group. Twenty-two false positive cases (22.2%) were observed in 99 of the normal group. One hundred and six cases out of the 130 cases (81.5%) satisfied this criterion. Therefore, this criterion was concluded to be useful to predict the coronary artery involvement in the earlier stage of the illness.

Age Factors↗
PubMed1984

Computer application for respiratory data processing in closed circuit anesthesia.

A computerized respiratory monitor system for closed circuit anesthesia is described. The system consists of a mass spectrometer, a hot-wire respiratory flow meter, a microcomputer, a central display and a recorder. Respiratory parameters obtained by the computerized system include O2 consumption, carbon dioxide production, expiratory minute volume and respiratory rate. Using this system, closed circuit anesthesia was used in eleven surgical patients. Breath by breath value of carbon dioxide production stayed within the normal range during the procedure, though that of oxygen consumption varied markedly. This system allows anesthesiologists to detect, breath by breath, optimal gas flow and metabolic requirement during the closed circuit anesthesia. Closed circuit anesthesia is being used with increasing frequency in recent years. The patient's condition must be monitored continuously during the closed circuit anesthesia to maintain optimal depth of anesthesia and to prevent hypoxia or hypercarbia; however, monitoring of the respiratory gas exchange is difficult. We have developed a computerized respiratory monitor system for the closed circuit anesthesia.

Adult↗
PubMed1984

Plasma vasopressin response to extracorporeal circulation in children.

Plasma vasopressin levels were measured during open-heart surgery in nine children. The control value of vasopressin was 5.5 +/- 1.5 (s.e.mean) microU/ml. During extracorporeal circulation, a maximum 7.3-fold increase of plasma vasopressin level was demonstrated. This high level of vasopressin was considered to have circulatory effects rather than anti-diuretic effects.

Age Factors↗