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Biomedical subjects

H Ishida

Publications and source records attributed to H Ishida.

At least 811 records · Page 45Linked to original sources
PubMed1988

Dual effects of clonidine on amylase secretory responses of rat parotid tissue induced by beta- and alpha 1-agonists.

The effects of clonidine on the amylase secretory responses of rat parotid tissue were examined in vitro. Clonidine increased amylase secretion induced by the beta-adrenergic agonist isoproterenol and decreased that induced by the alpha 1-adrenergic agonist methoxamine or phenylephrine with propranolol. Clonidine itself had no significant effect on the secretion. Yohimbine prevented the effects of clonidine on the secretions induced by both adrenergic agonists. Clonidine did not have any significant effect on cyclic AMP accumulation in the tissues induced by the beta-adrenergic agonist. The parotid tissue after reserpine treatment exhibited supersensitivity to beta- and alpha 1-adrenergic agonists. In reserpine treated tissues, the stimulatory effect of clonidine on beta-agonist-induced secretion disappeared, but the inhibitory effect on alpha 1-agonist-induced secretion remained unchanged. Similar results were obtained after sympathectomy of the gland. These data suggest that the inhibitory effect of clonidine on alpha 1-agonist-induced secretion is related to a postsynaptic site, whereas its effect on beta-agonist-induced secretion is still unknown.

Acetylcholine↗
PubMed1988

Circulating levels and bone contents of bone gamma-carboxyglutamic acid-containing protein are decreased in streptozocin-induced diabetes. Possible marker for diabetic osteopenia.

To investigate the pathophysiology of diabetic osteopenia, circulating levels and bone contents of bone gamma-carboxyglutamic acid-containing protein (BGP) were measured in streptozocin-induced diabetic rats . Plasma calcium and total protein were significantly decreased (P less than .01) in the diabetic group, and the plasma level of BGP in diabetic rats was 19.6 +/- 2.8 (mean +/- SE) ng/ml, which is significantly lower than the value of 89.2 +/- 14.0 ng/ml in control rats (P less than .01). Bone contents of calcium and hydroxyproline per femur were significantly decreased in the diabetic group (P less than .01), and the ratios of bone calcium to hydroxyproline were not different. Bone BGP content per femur in the diabetic group was 669 +/- 58 micrograms, which was also significantly lower compared with 1241 +/- 126 micrograms in control rats (P less than .01). The decreased bone content of BGP is consistent with the hypothesis that BGP synthesis is impaired in insulin-deficient diabetes. Because a relationship between plasma levels of BGP and bone turnover has been established, the low plasma BGP value suggests there is a decrease in bone turnover in diabetic rats. Therefore, we postulate that the low bone turnover is one of the pathological features of diabetic osteopenia and is at least partly responsible for the occurrence of this complication in diabetes mellitus.

Animals↗
PubMed1988

Minerals and vitamin-A status in relation to gustatory functions for salt in healthy female college students.

Twenty-five healthy female college students were studied for the gustatory function tests for salt (NaCl) and some selected biochemical parameters including plasma retinol and plasma retinol-binding protein (PRBP). Plasma zinc (PZn) and retinol levels were comparable with those of good responders in tests of discrimination between two levels of NaCl in the previous report, but PRBP was lower in the present subjects. From the results of correlation analysis and stepwise multiple regression analysis, the individual student's discriminability of NaCl concentrations was related to the parameters regarding metabolic status of calcium (Ca), magnesium (Mg), sodium (Na), and selenium (Se). The detection threshold of NaCl concentration ranged from 1 to 60 mmol/liter and was related to PRBP. Plasma Zn was significantly positively correlated with both plasma retinol and PRBP, but significantly negatively correlated only for the detection threshold of NaCl concentration. On the basis of these results, the importance of vitamin-A nutrition and the relation of minerals such as Na, Ca, Mg, and Zn to the gustatory functions of NaCl was confirmed and a possible participation of Se to the functions was suggested.

Adult↗
PubMed1988

[A monoclonal antibody, KM10 reactive with human gastrointestinal cancer].

A monoclonal antibody, KM10 (IgG1) was produced by fusing spleen cells from a human gastric cancer cell-primed BALB/c mouse with the murine myeloma cell line X63-Ag8-653. The antibody reacted strongly with the plasma membrane of human gastro-intestinal carcinoma. Sections of both malignant and benign tissues were tested with immunoperoxidase. Ten of 10 (100%) large intestine cancers, 26 of 31 (84%) gastric cancers, 5 of 7 (71%) pancreatic cancers and 3 of 3 (100%) ampulla of Vater cancers reacted positively. Moderate or little reactivity was observed with normal human tissues and carcinomas of the liver, mammary, thyroid and adrenal glands. According to a study of the distribution of 125I-labeled KM10 in nude mice bearing human gastric cancer, KM10 selectively localized in tumor tissue rather than normal tissue. Whole-body autoradiography also supported such selective distribution. By enzyme treatment and Western blot analysis, the antigenic determinant of KM10 antigen was demonstrated to be protein with a MW of about 240,000.

Animals↗
PubMed1988

Alterations of the central noradrenergic system in MPTP-induced monkey parkinsonism.

Alterations of the coeruleo-cortical adrenergic system in 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced monkey parkinsonism were examined through neuropathological observation and radioreceptor assay. Neuronal degeneration was found not only in the substantia nigra but also in the locus coeruleus of all the MPTP-treated monkeys. From radioreceptor assay, decreases of alpha 1 and alpha 2 receptors in the parietal and temporal cortices were observed in parkinsonian monkeys. In addition, beta receptors increased in the temporal cortex. These changes in monkey parkinsonism are very similar to those in human Parkinson's disease.

1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine↗
PubMed1988

[Endocrinological and metabolic disorders in the patients with massive bowel resection in the newborn period].

Massive (70%) resection of the small bowel was performed in seven newborn infants. Follow-up study was undertaken in four of these patients and the growth and endocrinological status were evaluated. Body weight and height of three patients were below 50th percentile. On glucose tolerance test, peak IRI was low and insulinogenic indices were below normal range. These findings suggest that there are some mechanisms which suppress the secretion of insulin. Pituitary and thyroid function were within normal limits but secondary sexual manifestation was not seen in a fifteen year old boy. Hyperoxaluria was seen in three children, and one had kidney stone with some impairment of renal function.

Adolescent↗
PubMed1988

[Two cases of cloacogenic carcinoma of the anal canal: chemotherapeutic effect of cis-dichlorodiammine-platinum].

Cloacogenic carcinoma, originating from the remnant of the cloaca, is a rare malignant tumor, comprising 2-3% of cancers of the anal canal. We reported here two cases of cloacogenic carcinoma whose pulmonary and hepatic metastases were successfully treated with CDDP administration, in spite of limited effect on primary lesions of the rectum. The promising usage of CDDP for cloacogenic carcinoma, especially for distant metastasis was discussed.

Anus Neoplasms↗
PubMed1988

Clinical application of autologous cultured epithelia for the treatment of burn wounds and burn scars.

This report presents our experience with autologous cultured human epithelia grafting on burn wounds, burn scars, and skin-graft donor sites in seven patients. Dispersed epidermal cells were cultured with 3T3 cells treated with mitomycin C. After 2 to 3 weeks, cultured epithelia (total 350 to 2250 cm2) were grafted to the wound. The results showed that cultured epithelia grafts did not take so completely compared to the meshed skin grafts used for the coverage of burn wounds. However, cultured grafts placed on aseptic wounds adhered well and showed good appearance. In the histologic findings, normal differentiation of epidermal cells was found. Cultured grafts were bordered from subepidermal granulative tissue with basement membrane. A rete ridge and the adnexal structures were absent in the specimens that adhered to the burn wounds. However, in the specimens that took on abraded wounds, a gently sloping rete ridge and elastic fibers were seen. The histologic findings showed structures resembling normal skin.

Adult↗
PubMed1988

[A clinical experience of upper urinary tract disease using an endourological technic].

Fifty-three patients with upper urinary tract disease were treated by using endourological technique between July, 1986 and July, 1987; percutaneous nephrolithotripsy (PNL) in 26 cases, transurethral ureterolithotripsy (TUL) in 11 cases, endopyelotomy for ureteropelvic obstruction in 3 cases, dilation of ureteral stenosis in 3 cases and percutaneous nephrostomy in 10 cases. The stones could be successfully removed in 24 cases (92%) of PNL and 10 cases (91%) of TUL. No severe complications were seen in this series. The many reports of PNL and TUL were investigated in detail, but other endourological approach will need further long-term followup.

Constriction, Pathologic↗