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Biomedical subjects

H Ise

Publications and source records attributed to H Ise.

At least 55 records · Page 3Linked to original sources
PubMed1980

A method of HRP bathing of transected peripheral nerves using a rubber bag.--Its technique and advantage.

The horseradish peroxidase (HRP) bathing method of transected peripheral nerves using a small rubber bag is described and its advantage for labeling neurons is evaluated. In order to apply the HRP to the peripheral nerves, a small rubber bag of appropriate size for the nerves to be bathed was made and was filled with a solution of 20% HRP. The proximal stump of the transected nerve was then inserted into the rubber bag. Nerve cell bodies, retrogradely labeled by this method, were observed to be intense. HRP diffusion to the surrounding tissues was considered negligible at the bathing region. The method presented in this paper is useful adjacent to methods previously reported for HRP application to the peripheral nerves. To illustrate this method, the results of the HRP bathing of either the chorda tympani or the intermediofacial nerve in cats were described.

Animals↗
PubMed1980

Identification of the phrenic nucleus in the cat as studied by horseradish peroxidase bathing of the transected intrathoracal phrenic nerve.

Horseradish peroxidase bathing of the transected intrathoracal phrenic nerve was done to identify the exact distribution of neurons of the phrenic nucleus in the cat. Retrogradely labeled neurons were observed in the ipsilateral ventral horn from the lower one-fourth of the 4th to the lower end of the 6th cervical segment. In the 4th segment, labeled neurons were found at the medial part of the ventrolateral cell group. Caudalwards, the neurons shifted from lateral to ventromedial locations and, in the 6th segment, the neurons merged into the lateral part of the ventromedial cell group.

Animals↗
PubMed1979

Identification of the inferior salivatory nucleus in the cat as studied by HRP bathings of the transected glossopharyngeal nerve root.

The localization of the inferior salivatory nucleus that gives rise to parasympathetic fibers to the parotid gland was identified by means of horseradish peroxidase (HRP) method in the cat. The inferior salivatory nucleus does exist in the medulla oblongata and is situated in the dorsal part of the reticular formation. The nucleus is well-circumscribed caudally but rostrally the nucleus becomes scattered within the wide area of the dorso-lateral reticular formation. The inferior salivatory nucleus, demonstrated by the present study is composed of medium-sized multipolar neurons with well-developed slender dendrites and densely stained Nissl substance.

Animals↗
PubMed1979

Identification of the superior salivatory nucleus in the cat as studied by the HRP method.

Using horseradish peroxidase (HRP) bathing of either the intermediofacial nerve or the chorda tympani, the localization of the superior salivatory nucleus that gives rise to parasympathetic fibers to the submandibular and sublingual glands was identified in the cat. The superior salivatory nucleus demonstrated by this study does not exist in the pons but does exist in the dorsal part of the reticular formation of the rostral medulla oblongata. Neurons of this nucleus were generally medium-sized and multipolar, with densely stained Nissl substance.

Animals↗
PubMed1979

Prognostic value of carotid angiography in severe head injury.

Seventy-seven carotid angiograms in patients with severe head injuries were reviewed. The prognostic value of angiographic findings was evaluated. We were particularly concerned with midline shift, extracerebral avascular area, cerebral circulation time, phlebographic index, and distance between insula and inner table of the skull. Poor visualization of the internal cerebral and the thalamostriate veins, and prolongation of the arterial circulation time were significantly related to a poor prognosis.

Adolescent↗
PubMed1979

Egg lectin of Rana japonica and its receptor glycoprotein of Ehrlich tumor cells.

Egg lectin of Rana japonica, which specifically agglutinates transformed cells but does not agglutinate nontransformed cells and erythrocytes, has been isolated by gel filtration and successive ion-exchange chromatographies on diethylaminoethyl cellulose and carboxymethylcellulose columns and has been characterized as a homogeneous carbohydrate-free protein with a relative molecular weight of 13,500. The lectin, at a concentration of 1 microgram/0.1 ml, causes obvious cytoagglutination of various transformed and tumor cell. The receptor of the Erlich ascites tumor cells which inhibits the lectin-induced agglutination of the Ehrlich ascites tumor cells has been isolated and characterized. The receptor was solubilized from Ehrlich ascites carcinoma cells by treating a tumor cell suspension with insolubilized trypsin, and the solubilized receptor was isolated by gel filtration through Sephadex G-100, followed by ion-exchange chromatography on diethylaminoethyl cellulose. The receptor was identified as a homogeneous glycoprotein having about 25% carbohydrate. The receptor, at a concentration of 4 microgram/0.1 ml, completely inhibited the cytoagglutination of the Ehrlich carcinoma cells caused by three agglutination doses (about 3 microgram/0.1 ml) of the R. japonica lectin.

Animals↗