Search PubMed⌕ Search

Biomedical subjects

H Inoue

Publications and source records attributed to H Inoue.

At least 1,243 records · Page 69Linked to original sources
PubMed1993

Ultrastructure and X-ray microanalysis of epiphyseal growth cartilage of femoral head processed by rapid-freezing and freeze-substitution.

Epiphyseal growth cartilage of the femoral head obtained from Wistar rats was investigated after fixation by a rapid-freezing and freeze-substitution. Liquid helium was used in order to achieve a fast cooling rate without ice-crystal damage during the rapid freezing. Use of the rapid-freezing and freeze-substitution procedure provided better ultrastructural preservation of the chondrocyte than conventional chemical fixation methods. This procedure allowed a more reliable approach to electron probe analysis. X-ray microanalysis of the specimens confirmed that calcium is not detected in the initial matrix vesicles as a result of the freezing process. The results suggest that calcium release from precipitates occurs in the free state without any detectable formation of hydroxyapatite at the initial stage of calcification and that calcium is not tightly bound to the matrix vesicles.

Animals↗
PubMed1993

Influence of Mn ion the action of dibutyryl cyclic AMP and forskolin on contraction, membrane response, and cyclic AMP-dependent protein kinase activity in rat myometrium.

Contractile response, membrane activity, and protein kinase A (PKA) activity were measured on the longitudinal muscle taken from the estrogen-treated rat uterus, and the influence of Mn ion on the inhibitory effects caused by db cAMP and forskolin was investigated. Phasic contractions generated in the muscle taken from the middle portion of uterus were depressed to 48 and 83% by 30 microM db cAMP and 0.1 microM forskolin in Mg-free Krebs solution, respectively; phasic contractions were more strongly depressed by the agents in the solution containing 0.2 mM Mn. Action potentials consisted of spike and plateau components, and the duration of the plateau potential was reduced by the application of the agents; membrane activity was more strongly depressed in the presence of 0.2 mM Mn. The contractile depression caused by db cAMP was reduced and by forskolin was enhanced by pretreatment of the tissue with 0.6 mM Mn for 30 min. The PKA activity was increased by 39 and 6% of the control, when 30 microM db cAMP and 0.1 microM forskolin were applied, respectively; the PKA activity in response to db cAMP and forskolin was reduced and enhanced, respectively, when the tissues were pretreated with 0.6 mM Mn. It was proposed that Mn ions permeated into cell interior when the muscle was exposed to 0.6 mM Mn, so that the effects of the agents were differently affected. It was also shown that plateau potential dominated in the muscle taken from the ovarian portion, and the contractile inhibition caused by the agents was far weaker.

Action Potentials↗
PubMed1993

Incidence and prognosis of dysvascular amputations in Okayama Prefecture (Japan).

This survey analysed the clinical characteristics of subjects who first underwent major amputation of lower limbs necessitated by dysvascular disease during the 5 year period from 1984 to 1988. All were residents of Okayama Prefecture, Japan, and have been issued with a Physically Disabled Person's Certificate. In total, 114 dysvascular amputees, representing 58.2% of all lower limb amputations performed in the resident population during the study period, were surveyed. The underlying diagnosis was arteriosclerotic obstruction in 64.9% of the subjects, diabetic gangrene in 22.8%, acute embolism in 7.0% and Buerger's disease in 5.3%. The yearly incidence of new dysvascular amputees per 100,000 people was estimated to be 1.2 among the general population and 5.7 among those aged over 65 years. At three years after primary amputation, the survival rate was 52.3% in arteriosclerotic obstruction, and 66.7% in diabetic gangrene. Secondary amputation was performed in 17.0% of the entire group. The concurrent incidence of hemiplegic stroke was 19.8%. Among 36 amputees due to arteriosclerotic obstruction, who survived 3 years postoperatively, 10 (27.8%) were fitted with prosthetic limbs.

Adult↗
PubMed1993

[Anti-Hp antibody may vary with strain used as antigen possibly due to serological diversity].

Western blotting and ELISA techniques have been used to investigate antigen specificity of systemic responses. Immunoblotting studies demonstrate several major protein antigens that are detected most sera. H. pylori immunoblotting studies using rabbit hyperimmune sera identify several distinct strains. Seven monoclonal antibodies which are prepared in our laboratory recognize 33-35 kDa and 66 kDa of H. pylori. ELISA studies using the monoclonal antibody reveals considerable antigenic diversity among H. pylori strains. Serotyping of clinically isolated H. pylori Seems to be useful in clarifying the etiological roles of this bacteria. In spite of studies by several investigators, serotyping of H. pylori has not been established yet. Our studies suggest that H. pylori need to be further subdivided serologically.

Animals↗
PubMed1993

Invasive human T lymphoma cells produce a novel factor that upregulates expression of adhesion molecules on endothelial cells.

The interaction between lymphoma cells and vascular endothelial cells (EC) is the first critical step in the invasion of lymphoma cells. We found that invasive human CCRF-CEM T lymphoma cells (CEM) released a factor that upregulates the expression of adhesion molecules on vascular EC. The supernatant of CEM (CEM-SUP) increased the expression of both ICAM-1 and ELAM-1 in time- and dose-dependent manners as shown by cell enzyme-linked immunoabsorbent assay (ELISA). In contrast, the induction of VCAM-1 on EC with CEM-SUP was relatively weak. No activity for interleukin-1 alpha (IL-1 alpha), IL-1 beta, interferon-gamma (IFN-gamma), or tumor necrosis factor-alpha (TNF-alpha), which are known to augment ICAM-1 expression, was detected in CEM-SUP by ELISA. In reverse transcriptase polymerase chain reaction (RT-PCR) assay, CEM expressed a minimum amount of TNF-alpha mRNA, but absolutely no IL-1 beta or IFN-gamma mRNA. In addition, antibodies for cytokines did not inhibit the upregulatory effect of CEM-SUP. Semipurified CEM-SUP further increased the cellular binding between CEM cells and EC in vitro. This factor was stable to heat (65 degrees C, 30 minutes) and labile to acid (pH 2.0). Gel filtration and chromatofocusing estimated its molecular weight at 50 kd, with an isoelectric point of pH 7.2. Production of this factor might contribute to the invasive character of CEM through upregulation of adhesion molecules on EC.

Base Sequence↗
PubMed1993

[Anticancer and side effect of arterial subsegmental chemoembolization using cisplatinum/pirarubicin lipiodol suspension (CTLS) for hepatocellular carcinoma].

Twenty patients with unresectable hepatocellular carcinoma were treated by intra-arterial subsegmental injection of Cisplatin/4-0-Tetrahydro-Pyranyl-adriamycin Lipiodol suspension (CTLS). The mean single doses of Lipiodol, cisplatin and THP were 2.3 ml, 85 mg and 8.9 mg, respectively. The therapy was given once in 10 patients, twice in 8 and 3 times in two. Over 25% reduction in tumor size was recognized in 12 patients (60%). Fifty or more % decrease of alfa-feto-protein (AFP) was observed in all of 7 patients (100%) with the initial serum AFP level of more than 200 ng/ml. Although transitional and mild symptoms, such as fever, abdominal pain and vomiting were recognized in some cases, no severe complications were encountered. This method is promising as an excellent procedure for unresectable hepatocellular carcinoma.

Aged↗
PubMed1993

[Neurogenic inflammation].

Airway neurogenic inflammation is caused by neuropeptides released from airway sensory nerves, and may be involved in the pathogenesis of asthma. Airway sensory nerves are stimulated by chemical mediators such as bradykinin and leukotrienes, hyperventilation and cigarette smoke. Presynaptic modulation of airway sensory nerves by opioids, histamine H3-agonists, NPY, and potassium channel openers is effective in the control of neurogenic inflammation. Tachykinin receptor antagonists also inhibit airway neurogenic inflammation. Recently, we have demonstrated that pretreatment with a tachykinin receptor antagonist can protect against bradykinin-induced airway narrowing in asthmatic patients. This evidence indicates that endogenously released tachykinins have a functional role in the airways of asthmatics.

Asthma↗
PubMed1993

Incidence of ultrasonographically-detected thyroid nodules in healthy adults.

We studied the incidence of thyroid nodules in healthy adults, who had no history of thyroid disease, in Tokushima, Japan, where goiter is not considered endemic. Examination was performed by palpation and using a 10-MHz real-time sonographic scanner. Palpable thyroid nodules were present in two (0.9%) of 227 men and in four (1.8%) of 224 women. On the other hand, nodules were detected by ultrasonography in 42 men (18.5%) and 47 women (21.0%). There was no significant difference in the incidence detected by ultrasonography between men and women in any of the age groups, except in men above 60 years old. It is concluded that thyroid nodules are not an uncommon disorder even in men. Since, clinically, the incidence of nodularity appears to be higher in women than in men, there may be difference between men and women in the development of nodules until they become clinically detectable.

Adult↗
PubMed1993

Long-term time-lapse cinemicrography of human erythroblasts under compression and without compression--direct measurement of generation time of each maturation stage.

The in vitro proliferation and the successive morphological alterations of single human erythroblasts were recorded for 5 days by time-lapse, phase-contrast cinemicrography. To visualize their fine structures more clearly, we introduced a new device to compress the erythroblasts under a cover slide. Appropriate compression did not significantly affect the capacity of the erythroblasts to proliferate and differentiate, although compression produced a slight tendency towards shorter generation time. The directly measured generation time was 24.5 +/- 4.5 h (mean +/- S.D.) for generation -1 (one generation prior to the orthochromatic erythroblast stage); 18.4 +/- 3.0 h for generation -2; and 18.3 +/- 4.1 h for generation -3 [corrected], these being arranged in inverse order of cell lineage. The cell size became progressively smaller and the nucleus-to-cytoplasm ratio decreased along with the maturation of the erythroblasts, showing a distinctive distribution range for each generation. Progression of hemoglobinization was simultaneously recorded qualitatively by Soret band absorption micrography. Cell movements characteristic for each maturation stage, e.g. perimitotic bleb-throwing of immature erythroblasts and vigorous movements of orthochromatic erythroblasts, heralding enucleation, were also clearly recorded. The method described here will be useful for the direct study of the dynamic morphology of the erythron in normal and abnormal states.

Cell Differentiation↗
PubMed1993

Ewing's sarcoma: evaluation of chemotherapy in 17 cases.

Seventeen patients with Ewing's sarcoma were divided into 3 groups according to treatment. Ten patients were treated with complete chemotherapy; 9 with VACA protocol, composed of vincristine, actinomycin-D, cyclophosphamide and doxorubicin, and the remaining 1 with T-11 protocol. Four were given incomplete VACA protocol, and another 3 received no chemotherapy. Clinical results were evaluated in the three treatment subgroups described above. All patients without systematic chemotherapy died from lung metastasis within 1 year of the initial treatment. Three of 4 patients who were given incomplete chemotherapy died after a mean survival period of 17.7 months, and one developed cancerous pleuritis after 38 months. In the group receiving complete systematic chemotherapy, 5 of 10 patients are alive, and 2 have been disease-free for more than 3 years. Distant metastasis is likely when viable cells persist at the site of the primary tumor, even if the tumor size has been considerably reduced by preoperative chemotherapy. Therefore, immediate initiation of intensive chemotherapy and maintenance for the full course of therapy can improve the prognosis of Ewing's sarcoma.

Adolescent↗
PubMed1993

Adjuvant chemotherapy for patients with soft tissue sarcoma.

Forty patients with soft tissue sarcoma (Enneking's stage I and II) were treated in our department between 1965 and 1992. We administered VACA regimen (composed of vincristine, actinomycin-D, cyclophosphamide, and doxorubicin) to 14 of 40 patients. Among 40 patients, the 5-year survival rate was 56% in 14 patients with chemotherapy and 39% in 26 without chemotherapy (p < 0.02). In 32 patients of Stage II, the 5-year survival rate was 40% in 11 patients with chemotherapy and 31% without chemotherapy (p < 0.05). The improvement of the survival rate was due to delay in the development of lung metastases (chemotherapy group: 21.2 months after first visit, non-chemotherapy group: 9.4 months) and prolongation of the survival period after metastases (chemotherapy group: 26 months, non-chemotherapy group: 7.4 months).

Adolescent↗
PubMed1993

[Effect of Ca-antagonists on pulmonary blood flow during single-lung ventilation in the dog].

Experiments were conducted in 21 adult mongrel dogs to clarify the effects of Ca-antagonist (nifedipine sublingual administration 2.5 mg or 5 mg, or nicardipine intravenous administration 30 micrograms/kg or 60 micrograms/kg) on pulmonary blood flow under the condition of collapsed lung on the side of thoracotomy. Under anesthesia, an endotracheal tube with a movable blocker was used to intubate the trachea and the thoracotomized lung was collapsed. Subsequently, a Ca-antagonist was given and the ratio of the left main pulmonary artery/ascending thoracic aorta blood flow was used as an index of hypoxic pulmonary vasoconstriction (HPV). The following results were obtained. 1. Ca-antagonist administration under lung-collapse on the thoracotomy side resulted in inhibition of HPV of the collapsed lung, whereas, arterial blood oxygen tension (PaO2) remained in the acceptable range. 2. The maintenance of the lowered PaO2 within the acceptable range may have been due to the increase in cardiac output and the elevation of mixed venous blood oxygen tension resulting from Ca-antagonist administration, as well as the residual HPV even after administration of the Ca-antagonist.

Animals↗
PubMed1993

[Effect of KSG-504, a new synthetic cholecystokinin receptor antagonist on ethionine-induced acute pancreatitis in rats].

KSG-504, a new synthetic cholecystokinin (CCK) receptor antagonist derived from proglumide, has superior selectivity and affinity to CCK-A receptors. We have investigated the effect of KSG-504 on ethionine-induced acute pancreatitis in rats and the influence of endogenous CCK on evolution of pancreatitis and regeneration of pancreatic acinar cells. Reduction of pancreatic proteins and digestive enzyme contents was dose-dependently prevented by subcutaneous administration of KSG-504. The inhibition of evolution of pancreatitis was demonstrated histologically in KSG-504 treated rats. The effect of KSG-504 on pancreatic regeneration was evaluated by bromodeoxyuridine labeling index (B.L.I.) of acinar cells. There was no significant difference of B.L.I. between KSG-504 treated and non-treated rats. These results suggest that KSG-504 has a beneficial effect on ethionine-induced acute pancreatitis by blockade of endogenous CCK.

Acute Disease↗
PubMed1993

Acute interstitial pneumonitis induced by carbamazepine.

A 62 year old man treated with carbamazepine for 3 months developed eczema and acute interstitial pneumonitis. A lymphocyte-stimulation test was reactive to carbamazepine. Withdrawal of the drug resulted in prompt improvement. Only 10 cases of this type of drug-induced lung disease have previously been reported.

Acute Disease↗
PubMed1993

Long-term cinemicrography of erythroblasts from a patient with congenital dyserythropoietic anemia type III: direct observation of dysplastic erythroblast formation.

Erythroblasts from a patient with congenital dyserythropoietic anemia type III (CDA III) were observed by time-lapse phase-contrast cinemicrography. The erythroblasts were compressed with a new mechanical device; this procedure enhanced the visualization of their structures. Soret band micrography was carried out simultaneously to assess cytoplasmic hemoglobinization. The proliferation and differentiation course of the majority of CDA III erythroblasts was similar to that of normal erythroblasts. The formation of dysplastic erythroblasts showed two patterns of progression: (1) Usually, the process began with the appearance of a well-demarcated clear area in the nucleus of immature erythroblasts. This clear area reappeared in the progeny of the immature erythroblasts, at an increased incidence and in increasing size and rigidity. It finally came to occupy most of the nucleus, compartmentalizing it. The maturing cells displayed vigorous movement and progression of hemoglobinization and the nuclei became lobular; they grew to the cell size at which most CDA III erythroblasts divide, but did not do so. (2) Less frequently seen were abnormalities in mitosis and cellular behavior (e.g., tri- or bi-polar mitosis and subsequent fusion of daughter cells) or rapid growth of one daughter cell with concomitant shrinkage of the other.

Anemia, Dyserythropoietic, Congenital↗
PubMed1993

[An experimental study on the relation between lung volume and pulmonary blood flow].

The purpose of this study was to elucidate whether or not the reduction of lung volume is related to the mechanism of blood flow reduction in the atelectatic lung. In adult mongrel dogs, whose main stem bronchus in the thoracotomized lung was obstructed, the change of blood flow in the lung containing N2 was compared with that in the collapsed lung. 1) Under a low partial pressure of O2 in the broncho-alveolar system by obstruction of the main stem bronchus, the blood flow did not change, even when the N2 volume in the lung was varied. Therefore, the change of blood flow in the collapsed lung is not attributable to the reduction of lung volume. 2) The lung blood flow increased when the partial pressure of O2 in the broncho-alveolar system was elevated by infusion of O2 into the collapsed lung, and it decreased with the subsequent O2 absorption leading to lung collapse. Therefore, the change of lung blood flow is due to the change of intraalveolar partial pressure of O2. 3) The reduction of blood flow in the atelectatic lung is caused by hypoxic pulmonary vasoconstriction, and is not influenced by the reduction of lung volume due to the lung collapse.

Animals↗
PubMed1993

New-style Univent with a light source on the blocker.

The author has introduced a new-style "endotracheal tube with a movable blocker" which has a transillumination light source on the front-most portion of the blocker. This device may obviate the need for bronchoscopic guidance of the blocker.

Bronchoscopes↗