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Biomedical subjects

H Inagaki

Publications and source records attributed to H Inagaki.

At least 199 records · Page 11Linked to original sources
PubMed1980

Respiratory changes of the P loop in the Frank vectorcardiogram.

(1) Frank vectorcardiographic P loops of 25 healthy males were recorded at deep inspiration and expiration by using a minicomputer system with an averaging technique. Respiratory changes in 21 P parameters were quantitatively investigated. (2) The maximal left component of the P loop, the magnitude of the horizontal maximal vector and the magnitude of the P polar vector significantly decreased at deep inspiration as compared with those at expiration. The frontal and the spatial maximal vectors were significantly deviated vertically at deep inspiration as compared to those at expiration. (3) It was considered to be better to obtain P loops at a particular phase of respiration or to use those obtained by an averaging technique in order to analyse P loop in detail in various heart diseases, especially in the exercise stress test associated with rapid deep respiration. (4) The mechanism of respiratory P loop changes was discussed.

Adult↗
PubMed1978

The determination of molecular weights of Streptomyces subtilisin inhibitor and the complex of Streptomyces subtilisin inhibitor and subtilisin BPN' by sedimentation equilibrium.

The molecular weight of Streptomyces subtilisin inhibitor (SSI), a protein proteinase inhibitor, and that of the complex of SSI and subtilisin BPN' [EC 3.4.21.14] were determined by a sedimentation equilibrium method in 25 mM phosphate buffer, at pH 7.0, ionic strength 0.1 M (NaCl), 25.0 degrees C. The molecular weight of SSI was found to be 23,000 over a wide concentration range, 0.01-10 mg/ml, the range used for inhibitory, spectrophotometric, and kinetic measurements. Based on the amino acid sequence, the molecular weight of SSI has been calculated to be 11,500 (Ikenaka, T., et al. (1974) J. Biochem. 76, 1191-1209); therefore, the molecular weight of 23,000 obtained above suggests that SSI is in a dimeric form under usual conditions in the concentration range of 5 X 10(-7)-5 X 10(-4) M. The molecular weight of the subtilisin BPN'-SSI complex was determined to be 78,000 in the concentration range of 0.03-5.0 mg/ml by sedimentation equilibrium of the crystallized preparation and by that of a mixture of subtilisin BPN' and SSI treated as a multicomponent-polydisperse system. The molecular weight obtained here, combined with the results of binding stoichiometry (Inouye, K., et al. (1977) J. Biochem. 82, 961-967) that showed that one mol of SSI (molecular weight, 11,500) and one mol of the enzyme (molecular weight, 27,500) are tightly bound (Kd less than 1 nM), demonstrate that one mol of dimeric SSI binds two mol of the enzyme to form a stable complex, E2I2.

Bacterial Proteins↗