Search PubMed⌕ Search

Biomedical subjects

H Hoshi

Publications and source records attributed to H Hoshi.

At least 343 records · Page 19Linked to original sources
PubMed1984

[Studies on serum CEA levels measured by enzyme immunoassay using monoclonal CEA-antibodies].

A double determinant enzyme immunoassay (EIA) using two monoclonal antibodies against CEA was established in the Abott laboratory. Serum CEA levels from patients with various cancers and benign diseases were measured using this EIA in comparison with the results obtained by radioimmunoassay (RIA) using polyclonal antibody against CEA. A significant correlation was noted between the values measured by the two methods. When serum CEA levels were more than 100 ng/ml, the values for EIA were found to be higher than those for RIA. However, this EIA was judged to be useful for monitoring cancer patients.

Antibodies, Monoclonal↗
PubMed1984

Selective elevation of the N1-acetylspermidine level in human colorectal adenocarcinomas.

The association of N1-acetylspermidine with human colorectal adenocarcinomas has been evaluated in this study. Free polyamines and their monoacetylated forms in adenocarcinomas, adenomas, and apparently healthy mucosae were determined using high-performance ion-exchange chromatography. The N1-acetylspermidine levels in well- and moderately differentiated adenocarcinomas were 27.30 +/- 3.13 (S.E.) (n = 99) and 22.86 +/- 3.60 (n = 22) nmol/g, wet weight, respectively. These values were significantly higher than those of benign adenomas (5.38 +/- 0.85 nmol/g, n = 31) and of control mucosae. The N1-acetylspermidine levels in control mucosae on the oral and anal side of adenocarcinomas were 5.84 +/- 1.44 (n = 57) and 7.92 +/- 2.89 (n = 50) nmol/g, respectively; no significant difference was observed between control mucosae and adenomas. The mean levels of three polyamines, putrescine, spermidine, and spermine in both adenomas and adenocarcinomas were about twice as high as those of control mucosae. The molar ratios of spermidine to spermine were significantly greater in both adenomas and adenocarcinomas than in control tissues. There was no obvious correlation between the free polyamine concentrations and the degree of malignancy of the colorectal tumors. These results suggest that the metabolism of N1-acetylspermidine in colorectal adenocarcinomas is quite different from that in adenomas and in nonneoplastic mucosae and that N1-acetylspermidine can be a promising biochemical marker of cancer in the human large intestine.

Adenocarcinoma↗
PubMed1983

Differential growth requirements of serum factors (bovine serum albumin and low density lipoprotein) for normal and transformed human cells in a serum-free culture.

Serum-free culture is a useful method for identifying the growth requirements of normal cells of human origin and comparing them to the requirements of transformed human cells. We examined differential growth requirements of serum factors (bovine serum albumin (BSA) and low density lipoprotein (LDL) on normal (HEL), Co-60 gamma ray-transformed (CT-1) and 4-nitroquinoline 1-oxide-transformed (SUSM-1) human fibroblast cells in the absence of serum. A decreased requirement for serum was closely related to a decreased requirement for BSA in both types of transformed cells. The growth rate and saturation density of HEL and CT-1 cells grown in serum-free medium supplemented with the optimal concentrations of BSA were almost equal to the rates of cells grown in serum-supplemented medium. When BSA alone was added to the serum-free medium it did not support the continuous growth of SUSM-1 cells because of cellular detachment. An addition of LDL to serum-free cultures not only promoted the moderate growth of HEL cells, it promoted the continuous growth of SUSM-1 cells as well. In contrast, LDL was not required for the optimal growth of CT-1 cells. Transformed human cells specifically had reduced quantitative and qualitative growth requirements for one or more serum factors.

Blood↗
PubMed1983

[Serum ferritin in patients with malignant neoplasms].

Serum ferritin levels were studied in 264 patients who were consisted of 153 patients with various malignant neoplasms and 111 patients with various benign diseases. Positive rate of serum ferritin in all patients with malignant neoplasms was 35%. Hepatomas and pulmonary cancer showed relatively high positive rate, respectively 65% and 42%. In patients with benign diseases, hepatic diseases showed the high positive rate (52%) and the other benign diseases was low positive rate (11%). The relationship between serum ferritin and alpha-feto-protein in patients with hepatomas and other liver diseases was low. And the relationship between serum ferritin and CEA (carcinoembryonic antigen) in patients with malignant neoplasms of gastrointestinal tract was also low. It seemed that the measurement of serum ferritin levels will be of low value in the differentiation of the patients with malignant neoplasms.

Carcinoembryonic Antigen↗
PubMed1982

Hemoglobin Ai levels in hyperthyroidism.

Hemoglobin AI (HbAI) was studied in 21 cases of hyperthyroidism (H) and 33 control subjects (C). The correlation equations between fasting blood glucose (FBG, mg/dl, x) and HbAI (%, y) in the H and C groups were: y=0.07x+1.26 (r=0.5, p less than 0.05) and y=0.048x+2.23 (r=0.94, p less than p.001), respectively. Five cases in the H group showed a higher level of HbAI far outside the confidence of 5% level of significance calculated from correlation equation for the C group. The correlation equation for the sum of blood glucose (sigma BG, mg/dl, x) during GTT and HbAI (%, y) in the H group was y=0.003x+5.3 (r=0.64, p less than 0.01); in the C group y=0.005x+3.1 (r=0.88, p less than 0.001). sigma BG and HbAI showed a close correlation in both groups. According to the results of the GTT, the subjects, either C or H group, were divided into three sub-groups: normal (n), borderline (b) and diabetic (d). The correlation equation between triiodothyronine (T3, ng/dl, x) and HbAI (%, y) in the H group was calculated as y=0.003x+6.49 (r=0.58, p less than 0.05). T3 in the n subgroup was 331 +/- 90 ng/dl, in the b subgroup 362+/-166 ng/dl and in the d subgroup 621+/-227 ng/dl, and there was a statistical difference between the b and d groups.

Adult↗