Biomedical subjects

H Hof

Publications and source records attributed to H Hof.

At least 199 records · Page 11Linked to original sources

PubMed1975

Adjuvancy of streptococcal nucleic acids.

A study was performed to find out whether or not RNA and DNA isolated from Group A streptococcal cytoplasm does possess adjuvant activity. The findings obtained indicate that the adjuvancy of streptococcal RNA is very similar to that of poly I:C. The adjuvant activity of poly I:C is characterized by its capacity to increase the number of pre-existing hemolysin-producing spleen cells and by its ability to increase the development of 19S and 7S producers early in the 12-day-period of primary immune response. The adjuvancy of streptococcal DNA was considerably less pronounced than that of RNA. Neither poly I:C nor streptococcal RNA and DNA were found to be capable of increasing the process of priming for the secondary immune response.

Adjuvants, Immunologic↗

PubMed1975

Studies on the immunizing capacity of orally administered particulate antigens. I. The efficiency of killed Bordetella pertussis cells.

The single i.p. injection of 2.5 times 10-8 killed B. pertussis cells protected 23 out of a group of 24 NMRI mice (95.8%) against the subsequent intracerebral infection, whilst 13 out of 24 mice (54.2%) survived the intracerebral challenge with virulent B. pertussis cells after prior oral administration of 2.5 times 10-11 killed B. pertussis cells, as demonstrated by the mouse protection test. Similar treatment with non-specific substances, such as egg white and saline, did not result in any increase of resistance. Systemic anaphylactic hypersensitivity to bovine serum albumin could also be achieved, when either both the protein antigen and the B. pertussis vaccine were given by the oral route or when the B. pertussis vaccine was injected intraperitoneally into mice which had received the soluble protein antigen by the oral route. Such effects were not produced at all in the reverse situation, when the B. pertussis vaccine was orally administered in mice, which were given the soluble protein antigen by the intraperitoneal route. After oral inoculation of 6 times 10-11 killed B. pertussis cells neither splenomegaly nor blood lymphocytosis became detectable. It is still unknown, in which manner the orally administered B. pertussis vaccine effects protection against the intracerebral infection with virulent bacteria as well as susceptibility for systemic anaphylaxis. The data presented do not favor the view that those effects are due to the phenomenon of persorption.

Administration, Oral↗

PubMed1975

Influence of Bordetella pertussis and bacterial endotoxins on the immunological reactivity of germfree mice.

As compared to specifically pathogen-free NMRI mice, in principle, the immunological reactivity of germfree mice of the same strain and age was not found to be reduced. This is documented by the cellular kinetics of the primary immune responses, evoked by the intraperitoneal (i.p.) injection of either a "saturated" dose of 4 times 10(8) sheep erythrocytes (SE) or the simultaneous injection of 4 times 10(8) SE and 3 times 10(9) killed Bordetella pertussis organisms (PO). Thereby, adjuvancy of PO was not found to be reduced in germfree mice. The only difference consisted in the demonstration of significantly reduced numbers of both direct and indirect plaque-forming spleen cells (PFC) on the 4th day after primary antigenic stimulation. This is suggested to be due to a lack of sufficient training of the immunological apparatus of germfree mice. Both in germfree and conventional mice significant splenomegaly, blood leukocytosis as well as increase in the numbers of pre-existing "background" PFC became detectable following a single i.p. injection of 3 times 10(9) PO without SE. Similarly, the injection of endotoxin from Serratia marcescens produced a moderate increase in the numbers of "background" PFC. From the data presented it is suggested that strict gnotobiotic conditions do not cause noteworthy deficiency in immunological competence.

Adjuvants, Immunologic↗

PubMed1974