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Biomedical subjects

H Heimpel

Publications and source records attributed to H Heimpel.

At least 271 records · Page 15Linked to original sources
PubMed1978

Human placenta-conditioned medium for stimulation of human granulopoietic precursor cell (CFU-C) colony growth in vitro.

Human placenta-conditioned medium (HPCM) has been reported to stimulate colony formation by human granulopoietic stem cells (CFU-C) in vitro. The present work was performed to further characterize this colony formation. The majority of HPCM batches tested stimulated colony growth equivalent to recombined human leukocyte feeder layers with optimal cellular composition. A broad plateau of the dose-response curve of HPCM was found. A linear correlation exists between the number of marrow cells plated and the number of colonies grown. Optimal duration of culture is between 9 and 11 days. Colonies are large and tend to be compact. Admixture of mature granulocytes does not affect the colony growth pattern under optimal culture conditions. These data document that HPCM is a suitable source of colony-stimulating activity for the routine assay of human CFU-C. Due to the constant colony stimulation, HPCM appears particularly valuable for longitudinal studies of human CFU-C.

Colony-Stimulating Factors↗
PubMed1978

[Progressive pulmonary fibrosis due to combined treatment with BCNU, cyclophosphospahmide and cytosin-arabinoside (author's transl)].

Two patients treated for acute leukaemia with BCNU, cyclophosphamide and cytosin-arabinoside are reported, in whom pulmonary fibrosis developed and progressed during therapy. The development of lung fibrosis during combined treatment, together with serological exclusion of other diseases known to be associated with pulmonary fibrosis, make a causal connection between the treatment and the fibrosis very probable.

Carmustine↗
PubMed1978

Changes of granulopoiesis during and after adjuvant chemotherapy of breast cancer.

Adjuvant chemotherapy allows a study of the effects of cytotoxic drugs on natural human haematopoiesis. We describe serial studies of granulopoiesis performed during and after intermittent adjuvant chemotherapy for breast cancer (adriamycin plus cyclophosphamide, given for six courses at monthly intervals). After drug administration, a sequential wave of depletion and regeneration through successive granulopoietic compartments was observed. With repeated chemotherapy, moderate neutropenia developed, and the blood CFU-C pool size became progressively reduced. After the sixth chemotherapeutic course, granulopoietic regeneration was delayed. Following discontinuation of chemotherapy, a long-lasting (greater than 200 d) reduction of the blood CFU-C pool size, together with neutropenia and reduction of marrow segmented neutrophils, was observed, suggesting a defect of granulopoiesis with persistent granulopoietic hypoplasia. In patients with expected long survival, residual bone marrow damage should be added to the list of potential late side effects of cytotoxic drug therapy.

Bone Marrow↗
PubMed1977

[Distribution of haemoglobin content in human red cells, estimated by scanning cytophotometry (author's transl)].

Haemoglobin content and surface area of human red cells were estimated using a scanning cytophotometer connected to a computer for registration and analysis of the data. The measurements were carried out on fixed, unstained peripheral blood smears at a wavelength of 414 nm. The scanning can be controlled on the screen in order to detect errors and to eliminate extinctions from other sources than the cell examined. The method allows to demonstrate the topographic quantitative distribution of haemoglobin within the cell, to estimate haemoglobin content and surface area in individual cells and to correlate these values, to establish frequency distributions of the data within cell populations and to calculate various statistical parameters. Results of measurements on normal and abnormal red cells (iron deficiency, haemolytic anaemias) are demonstrated. The method may be used for investigation on red cell pathophysiology and may serve as a basis for image analysis in blood smears. It is too time consuming for direct diagnostic application in clinical practice.

Anemia, Hemolytic↗
PubMed1977

The clinical efficacy of granulocyte transfusions: studies on the oral cavity.

Patients with severe granulocytopenia of different origin received granulocyte transfusions. The migration and phagocytic ability of the transfused granulocytes in the oral cavity ("orogranulocytes") served as a parameter for measuring the viability of the granulocytes in vivo. There was no difference between granulocytes collected by continuous flow centrifugation and those collected by filtration leukapheresis. Under the influence of the transfusion treatment mucous membrane ulcers (MMU) subsided and healed. Where ulcers were present the number of orogranulocytes (OG) increased after granulocyte transfusion. In addition there was a significant correlation between the number of granulocytes in the peripheral blood and the number of orogranulocytes. As a control, orogranulocytes were studied in a group of healthy persons with intact teeth, toothless persons with normal blood counts and one patient with an acute agranulocytosis.

Adult↗
PubMed1977

[First experience with the practical year in internal medicine (author's transl)].

The first experience with the training program carried out in the University of Ulm presents a number of problems which could be important for its realization in teaching hospitals. The seminar and course program should be related to practice and be organized with the active cooperation of the students. The quality of the training to a great extent depends on the didactic skill, willingness to teach and a not inconsiderable expense of time for the chief physician, the assistant chief physician and the physician in charge of the wards during visits and when working in the ward. The omission of the "Junior Intern" status seems to be a disadvantage to the teaching organization. Condensing the seminar sessions into one month in a three-month period is possibly a logical compromise to guarantee the necessary continuity of the ward work and additional theoretical preparation for the final examination which the student requires.

Education, Medical, Undergraduate↗
PubMed1977

Improvement of preparative conditions for cytogenetic studies by short-term liquid culture of haemopoietic cells.

In patients with haemopoietic failure, the low incidence of cells in mitosis often prevents successful chromosome preparations. By performing liquid cultures of myeloid cells a high incidence of mitoses could be obtained even in those cases failing to provide mitoses when the direct preparation technique was used. The cytogenetic studies demonstrated that the mitoses achieved by culture techniques were aneuploid and, thus, there is evidence that they are of leukaemic origin. The combination of short-term liquid culture and chromosome analysis appears to be a diagnostic approach in selected cases of hemopoietic insufficiency.

Acute Disease↗
PubMed1977

[Haematological features of anorexia nervosa (author's transl)].

Nine patients with anorexia nervosa were studied, who had varying degrees of bone marrow failure ranging from a slight neutropenia to severe pancytopenia. In addition to routine laboratory work bone marrow biopsies were performed at admission and during the course of disease. In four of those patients erythropoietin excretion per 24 hours was measured by the bioassay in the polycythemic mouse at least twice during the course of treatment. At admission most bone marrow showed a marked although varying hypocellularity with grossly apparant background gelatinous material, which appeared to consist of acid mucopolysaccharides. Leukopenia and the changes of the bone marrow morphology reversed to normal after refeeding. Erythropoietin excretion was only elevated in patients who were anemic and returned to normal when the haematocrit reached normal levels. It seems therefore unlikely that an impaired production of erythropoietin or other postulated humoral factors cause the haemopoietic changes in anorexia nervosa. A direct effect of fat or carbohydrate depletion and/or of the increased mucopolysaccharides upon the proliferation of the haemopoietic cells can be suggested, but is an entirely speculative preposition.

Adolescent↗
PubMed1977

[Immunoblastic lymphadenopathy--malignant neoplasia or hyperimmune reaction? (author's transl)].

Six patients have been observed which exhibited the features of the so called immunoblastic lymphadenopathy. The histological and clinical findings allow to distinguish the disease from both the malignant lymphomas and other forms of benign pseudolymphomas. The most important clinical features are severe general symptoms, generalised lymphadenopathy, hepatosplenomegaly, skin rash and a variety of abnormal reactions in the B-cell system such as hypergammaglobulinaemia, a transient positive Coombs test and the appearance of plasma cells in the peripheral blood. Evidence for a neoplastic nature of the disease is lacking. Nevertheless, the course of the disease may be progressive and fatal, but spontaneous remissions and subsequent relapses have also been essential part of the therapeutic strategy. Immunosupressants such as corticosteroids may have a positive effect in cases with immunological complications.

Adult↗