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Biomedical subjects

H Hayes

Publications and source records attributed to H Hayes.

90 records · Page 5Linked to original sources
PubMed1984

A high-molecular-mass cell wall protein released from Clostridium tyrobutyricum by heat treatment.

Analysis of the cell wall of 4 strains of Clostridium tyrobutyricum reveals an unusually high protein content (35-40% dry weight). Brief heat treatment of whole cells of these stains causes release of two proteins, flagellin and a cell wall component of high molecular mass (110-125 kDa in the different strains). This component represents approx. 5% of the dry cell weight.

Bacterial Proteins↗
PubMed1980

Simultaneous augmentation mammaplasty and correction of inverted nipples.

This case proceeds from a patient of ours complaining both of small breasts and of inverted nipples. A lengthy discussion was held with her regarding simultaneous repair of both deformities and the two basic types of correction available for inverted nipples. The decision was made to undertake the combined procedure. The result shows the feasibility of performing the two procedures in one stage through the same incision. We found no previous report of such an operation.

Adult↗
PubMed1979

Indentation tonometry of breasts.

We present an indentation tonometer for use in measuring the softness or firmness of human, female breasts. It provides objective measurements which are consistently reproducible.

Breast↗
PubMed1977

Spider bite on an augmented breast.

A patient who had had an augmentation mammaplasty two years previously sustained a spider bite on one breast. The treatment and the course of the recovery are described, and the latter is illustrated.

Breast↗
PubMedDate not supplied

Major protein components in the cell envelope of Clostridium tyrobutyricum.

The overall composition of the Clostridium tyrobutyricum cell envelope did not vary significantly during cell growth and was characterized by a high protein content (about 40% dry weight). Teichoic and teichuronic acids were absent and the neutral sugar content low. Insoluble peptidoglycan represented only 10-12% of the cell envelope (dry weight basis); it contained glucosamine, muramic acid, alanine, diaminopimelic acid and glutamic acid (molecular ratio 1/1/2/1/1). SDS-PAGE revealed the presence of about 50 proteins in this cell envelope; however, one high molecular weight protein was largely predominant. They were not covalently bound to the peptidoglycan and their relative amounts were practically constant through cell growth and with various extraction treatments. A brief heat treatment of whole cells in PBS caused selective release of the major cell envelope proteins together with flagellin; this method was used to characterize these proteins in 37 strains of C. tyrobutyricum and some other clostridia. The major envelope proteins had molecular weights ranging from 96 to 145 Kd and the flagellins from 32 to 72 Kd.

Bacterial Proteins↗
PubMedDate not supplied

The R- and G-banded karyotypes of the sable antelope (Hippotragus niger).

The karyotype of the sable antelope has been investigated by RBG- and GTG-banding techniques. Chromosome preparations were made from peripheral blood lymphocytes, and primary fibroblast cells were cultured from skin biopsies. The RBG-banded chromosomes were identified and classified according to the standard conventions used for cattle and goat. The diploid number of the sable antelope is 60. The autosomes are all acrocentric, the X is a large acrocentric, and the Y is a minute acrocentric. Comparison of banding patterns in sable antelope and cattle chromosomes reveals the existence of a high degree of homoeology except for at least one pair of autosomes and the X chromosome.

Animals↗
PubMedDate not supplied

Chromosomal evolution in gazelles.

The chromosomes of nine gazelle species and two other Antilopinae species (Antidorcas marsupialis and Antilope cervicapra) were prepared from fibroblast cultures. G- and C-band karyotypes were constructed, and when possible, autosomal arms were numbered according to the cattle standard karyotype. Diploid chromosome numbers ranged from 30 to 58. Based on band similarity, chromosome-arm homoeologies were extensive, whereas shared homoeologous biarmed chromosomes were rare. Therefore evolution in this genus could have occurred mainly by speciation following monobrachial homoeology of centric fusions. X to autosome translocations were common in the whole genus. Furthermore, chromosome Y was also involved in an autosome translocation in gazelles from the subgenus Nanger and in Gazella thomsoni and G. rufifrons. Based on these karyotypic data a phylogenetic tree is proposed. This phylogenetic reconstruction confirms most of the taxonomic relationships obtained by morphological analyses for this group of species. The main novelties are the proximity of G. rufifrons and G. thomsoni and the inclusion of Antilope cervicapra in the gazelle group.

Animals↗
PubMedDate not supplied

A review of modern concepts of healing of cutaneous wounds.

Basic-science aspects of wound healing are discussed in the light of recent developments in wound-healing research. The author discusses wound healing in six, arbitrarily chosen phases, namely, inflammation, epithelialization, vascularization, contraction, collagen synthesis and late remodeling of scars that occur simultaneously in concert, but in such a manner that enables one to distinguish clinically between healing by primary intention and healing by secondary intention.

Aged↗
PubMedDate not supplied

Development and assignment of bovine-specific PCR systems for the Texas nomenclature marker genes and isolation of homologous BAC probes.

In 1996, Popescu et al. published the Texas standard nomenclature of the bovine karyotype in which 31 marker genes, already mapped in man, were chosen to permit unambiguous identification and numbering of each bovine chromosome. However, specific PCR systems were not available for each marker gene thus preventing the assignment of part of these markers by somatic cell hybrid analysis. In addition, some difficulties remained with the nomenclature of BTA25, BTA27 and BTA29. In this work, specific PCR systems were developed for each of the marker genes except VIL1 (see results), from either existing bovine or human sequences, and a bovine BAC library was screened to obtain the corresponding BAC clones. These PCR systems were used successfully to confirm the assignment of each marker gene (except for LDHA, see results) by analysis on the INRA hamster-bovine somatic cell hybrid panel. The difficulties observed for LDHA and VIL1 are probably due to the fact that these genes belong to large gene families and therefore suggest that they may not be the most appropriate markers for a standardisation effort. This panel of BACs is available to the scientific community and has served as a basis for the establishment of a revised standard nomenclature of bovine chromosomes.

Animals↗
PubMedDate not supplied

Construction and characterization of a bovine BAC library with four genome-equivalent coverage.

A bovine artificial chromosome (BAC) library of 105 984 clones has been constructed in the vector pBeloBAC11 and organized in 3-dimension pools and high density membranes for screening by PCR and hybridization. The average insert size, determined after analysis of 388 clones, was estimated at 120 kb corresponding to a four genome coverage. Given the fact that a male was used to construct the library, the probability of finding any given autosomal and X or Y locus is respectively 0.98 and 0.86. The library was screened for 164 microsatellite markers and an average of 3.9 superpools was positive for each PCR system. None of the 50 or so BAC clones analysed by FISH was chimeric. This BAC library increases the international genome coverage for cattle to around 28 genome equivalents and extends the coverage of the ruminant genomes available at the Inra resource center to 15 genome equivalents.

Animals↗