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Biomedical subjects

H Hatano

Publications and source records attributed to H Hatano.

At least 145 records · Page 8Linked to original sources
PubMed1975

Epidermal surface saccharides reactive with phytohemagglutinins and pemphigus antigen.

Inorder to study the relationship between epidermal surface saccharides and pemphigus antigen(s), fluorescein-labelled Concanavalin A (Con A) and Phytohemagglutinin-P (PHA-P) were used. These phytohemagglutinins were found to bind with the intercellular areas of human epidermis. Alpha-methyl-D-mannoside and alpha-methyl-D-glucoside inhibited the epidermal intercellular staining pattern produced by ConA-FITC, while N-acetyl-D-galactosamine blocked the same staining pattern produced by PHA-P-FITC. Normal human skin reacted with pemphigus antibody and pemphigus skin with the deposition of IgG both gave a positive intercellular staining pattern with fluorescein labelled phytohemagglutinins. Our data indicated the non-identity of the binding of Con A and PHA-P, and pemphigus antigen(s).

Aged↗
PubMed1975

Epidermal intercellular binding of concanavalin a and pemphigus antibody.

The binding sites of Concanavalin A (Con A) and pemphigus antibody in the intercellular spaces of the human epidermis have been investigated. Extraction of human skin with phosphate-buffered saline (PBS) and 70% ethanol resulted in complete loss of the binding sites of the pemphigus antibody, whereas the extracted skin still retained its reactivity to Con A. Preincubation with more than 0.25 mg/ml of Con A with normal skin caused a reduction in the binding of pemphigus sera. However, preincubation of pemphigus sera with human skin caused no inhibition of the subsequent binding of fluorescein-labelled Con A. These results indicate that Con A binding sites might be related to, but not identical with those of pemphigus antibody.

Antibodies↗
PubMed1975

Serologic differences in strains of Sporothrix schenckii.

To obtain evidence that Sporothrix scheneckii enters the body by contact with contaminated materials, the antigenic property of strains from different sources was investigated. The reciprocal absorption test of the antisera against a soil isolate and a human isolate (KO 4606) showed that the absorbed antisera against KO 4606 possessed unique antigen(s) in addition to the common antigen of both strains. Twenty-three clinical isolates were tested with absorbed antisera. Not all of them possessed the unique antigen(s), but there were serologic varieties among S. schenckii strains, regardless of their sources, clinical type of the disease and the morphology of the yeast phase cells.

Antigens, Fungal↗