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Biomedical subjects

H Hashiguchi

Publications and source records attributed to H Hashiguchi.

44 records · Page 3Linked to original sources
PubMed1983

Macroporous hydrogel membranes for a hybrid artificial pancreas. II. Biocompatibility.

A histological evaluation of the biocompatibility of a new porous hydrogel membrane made of 2-hydroxyethyl methacrylate (2-HEMA) was performed. Isolated membranes and diffusion chambers made of this material and filled with rabbit pancreas were implanted in diabetic rats for periods of up to 8 1/2 months. Histologic evaluation showed that this polymer elicits minimal tissue reaction within the first 7 weeks but some increase in granulation tissue and fibrosis thereafter. A component of this tissue reaction is neovascularization, and many blood vessels were noted immediately adjacent to the membrane's surface. Calcification of the membrane was also progressive but could be minimized by formulations resulting in the incorporation of acid groups throughout the hydrogel and on its surface. Diffusion chambers made of this new material show promise for use in a "hybrid" artificial pancreas for the treatment of diabetes.

Animals↗
PubMed1983

Whole-organ incubation as a first step in the isolation of pancreatic islets from large animals.

Ninety-five porcine pancreases were incubated at temperatures of 0, 20, or 37 degrees C for periods of 1-32 h. After incubation at 37 degrees C for 2-4 h, islet cells retain their morphologic and functional integrity, whereas acinar cells become necrotic. Because of acinar destruction, the pancreas also becomes soft and amenable to mechanical separation with a simple new device. Temperature-controlled incubation therefore may be a useful first step in the isolation of islet cells from large animal pancreases.

Animals↗
PubMedDate not supplied

Effect of unilateral dentate nucleus lesions on amygdaloid kindling in rats.

Amygdala kindling is used to investigate cerebellar dentate nucleus participation in the neuronal plasticity of the cerebrum. We studied the behavioral change, the occurrence of epileptiform events, and the duration of afterdischarge during amygdaloid kindling under control conditions and after a dentate lesion of the contralateral cerebellum. Our results indicate that the dentate lesion induced the facilitation of the behavioral development of the amygdaloid kindling, whereas there was only a slight increase in the duration of afterdischarge and the spontaneous epileptiform potential of the amygdala. There was a discrepancy between the behavioral kindling and the electroencephalographic epileptic activity. Therefore the dentate lesion must play a role in controlling the generalization mechanism of epilepsy. We also found that on a right amygdala that had been influenced by a left dentate lesion there were few spikes, while on the left amygdala there were frequent spikes. These results suggest that the dentate nucleus plays an important role in amygdaloid kindling as well as in the neuronal plasticity of the amygdala.

Amygdala↗