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Biomedical subjects

H Haraguchi

Publications and source records attributed to H Haraguchi.

At least 55 records · Page 3Linked to original sources
PubMed1996

Cytotoxic and antioxidative sesquiterpenoids from Heterotheca inuloides.

Four sesquiterpenoids, beta-caryophyllene, beta-caryophyllene 4,5 alpha-oxide, 7-hydroxy-3,4-dihydrocadalin, and 7-hydroxycadalin, isolated from the dried flower of Heterotheca inuloides Cass. (Asteraceae), have been found to exhibit cytotoxic activity against several solid tumor cell lines. Among them, 7-hydroxy-3,4-dihydrocadalin and 7-hydroxycadalin have also been found to inhibit autoxidative and microsomal lipid peroxidation.

Animals↗
PubMed1996

Inhibition of lipid peroxidation by sesquiterpenoid in Heterotheca inuloides.

A sesquiterpenoid, 7-hydroxy-3,4-dihydrocadalin, isolated from a Mexican medicinal plant Heterotheca inuloides was evaluated as an antioxidant. This sesquiterpenoid inhibited mitochondrial and microsomal lipid peroxidation induced by Fe(III)-ADP/NADH or Fe(III)-ADP/NADPH. Furthermore, 7-hydroxy-3,4-dihydrocadalin protected red cells against oxidative haemolysis. This sesquiterpene was thus shown to be effective in protecting biological systems against oxidative stresses.

Animals↗
PubMed1996

Protection against oxidative damage by dihydroflavonols in Engelhardtia chrysolepis.

Dihydroflavonol taxifolin and its glycoside, astilbin, from Engelhardtia chrysolepis were evaluated as antioxidants and radical scavengers. These dihydroflavonols inhibited superoxide anion production in the xanthine/xanthine oxidase system. Microsomal lipid peroxidation induced by NADPH-cytochrome P-450 reductase was also inhibited by these flavonoids. Mitochondrial lipid peroxidation was inhibited only by the aglycon. Taxifolin protected peroxy radical-damaged mitochondria with no effect on enzyme activity. Furthermore, taxifolin and astilbin protected red cells against oxidative hemolysis. These dihydroflavonols were found to be effective for protecting subcellular systems and red blood cells against oxidative stress in vitro.

Animals↗
PubMed1996

Determination of rare earth elements in blood serum reference sample by chelating resin preconcentration and inductively coupled plasma mass spectrometry.

Rare earth elements (REEs) in a blood serum reference sample, which is a freeze-dried sample issued from the National Institute for Environmental Studies, Japan, have been determined by inductively coupled plasma mass spectrometry (ICP-MS) after sample digestion and concentration pretreatment. The freeze-dried serum sample (ca. 0.8 g), which corresponded to 10 ml of original blood serum, was digested with HNO3 with heating on a hot plate. The digested sample was then diluted with 100 ml of 0.1 M HNO3, and REEs in the diluted solution were adsorbed on chelating resin (Chelex 100). The resin was then filtered with a glass filter. Finally, REEs on the resin were dissolved with 10 ml of 2 M HNO3 aqueous solution, and the sample solution was analyzed by ICP-MS. The concentrations of all the REEs were successfully determined by the present method. The recovery values of REEs were in the range of 70-80%, and the relative standard deviation of the recovery values in repeated experiments (n = 3) was less than 5% for all REEs. In addition, 20 other elements were also determined by ICP-MS and ICP-AES.

Cation Exchange Resins↗
PubMed1995

Inhibition of lipid peroxidation and superoxide generation by diterpenoids from Rosmarinus officinalis.

Four diterpenoids, carnosic acid (1), carnosol (2), rosmanol (3), and epirosmanol (4), were isolated as antioxidative agents from the leaves of Rosmarinus officinalis by bioassay-directed fractionation. These diterpenoids inhibited superoxide anion production in the xanthine/xanthine oxidase system. Mitochondrial and microsomal lipid peroxidation induced by NADH or NADPH oxidation were also completely inhibited by these diterpenes at the concentration of 3-30 microM. Furthermore, carnosic acid protected red cells against oxidative hemolysis. These phenolic diterpenes were shown to be effective to protect biological systems against oxidative stresses.

Diterpenes↗
PubMed1995

[Fourteen cases of malignant melanoma of the nasal cavity].

Fourteen cases of malignant melanoma of the nasal cavity were treated in our department during 31 years from 1962 to 1993. Ten were males and 4 were females. The ages ranged from 48 to 92 years old, with an average of 64.6 years. The chief complaints were epistaxis in 10 cases, nasal obstruction in 7, nasal cavity tumor in 1, and dull headache in 1. Histologically, 3 cases were amelanotic type, 3 oligomelanotic and 8 melanotic. The cellular types were classified as follows: 5 spindle and 9 large epitheloid cell types. Palliative treatment was performed in 1 patient, and 13 patients were treated radically. Local recurrences were seen in 8 patients, 9 regions; 3 near the posterior margin, 1 near the upper margin, 1 cheek, 2 ethmoid sinus, 1 maxillary sinus, and 1 uncertain. The cumulative survival rate among the 14 patients was 54.2% after 2 years and 31.0% after 5 years. One patient had local recurrence 13 years after surgical treatment.

Aged↗
PubMed1995

Malignant tumors of the nasal cavity: review of a 60-case series.

Sixty cases of primary malignant tumor of the nasal cavity treated in our hospital between 1962 and 1993 were reviewed. Males were affected 2.8 times more frequently than females. The age at the first consultation ranged from 11 to 92 years, with a mean of 55.1 years. The peak distribution was seen in the 6th decade. Twenty-six cases were epithelial malignancies (squamous cell carcinoma 15; adenocarcinoma 3; adenoid cystic carcinoma 3; undifferentiated carcinoma 3; mucoepidermoid carcinoma 1; malignant mixed tumor 1), while 34 cases were non-epithelial malignancies (malignant melanoma 14; malignant lymphoma 14; plasmacytoma 3; olfactory neuroblastoma 2; rhabdomyosarcoma 1). The most common symptom on presentation was nasal obstruction (66.7%), followed by epistaxis (55.0%). The first recurrence was local in 19 patients, whereas cervical lymph node metastasis occurred in three patients, bone metastasis in two, intraperitoneal metastasis in two, and brain metastasis in one. The overall five-year cumulative survival rate was 48.0%. The five-year survival rates for squamous cell carcinoma, malignant melanoma, and malignant lymphoma were 57.0%, 31.0%, and 40.0%, respectively.

Adolescent↗
PubMed1994

Simultaneous separation of inorganic cations and anions by ion chromatography using a single column coated with weak/strong-charged zwitterionic bile salt micelles.

A single reversed-phase ODS (octadecyl silica) column coated with taurine-conjugated bile salt micelles has been investigated for simultaneous separation of inorganic cations and anions. Under acidic conditions in the separation column, taurine-conjugated bile salts are protonated; therefore, the stationary phase coated with taurine-conjugated bile micelles exists as a "weak/strong"-charged zwitterionic stationary phase (H+ and SO3-). The weak/strong-charged zwitterionic stationary phase provides a new retention mechanism which is different from conventional ion chromatography in that both positive and negative charges are close together in a single molecule, resulting in simultaneous electrostatic attraction and repulsion of the analyte ions. Also, the stronger negative charge (sulfonate) of the stationary phase works as a cation-exchange site at the same time. The combined effects of cation-exchange and simultaneous electrostatic repulsion/attraction interactions were successfully used in ion chromatography for the simultaneous separation of inorganic cations and anions.

Anions↗
PubMed1994

Flavonoids in Rosmarinus officinalis leaves.

Three new flavonoid glucuronides, luteolin 3'-O-beta-D-glucuronide, luteolin 3'-O-(4"-O-acetyl)-beta-D-glucuronide, and luteolin 3'-O-(3"-O-acetyl)-beta-D-glucuronide, together with hesperidin, were isolated from 50% aqueous MeOH extract of the leaves of rosemary. The structures were established by chemical and spectroscopic methods. Their antioxidant activities were evaluated by a ferric thiocyanate method with hesperidin showing the greatest activity.

Antioxidants↗
PubMed1994

Immunohistochemical study of the cytogenesis of prolactin and growth hormone cells in the anterior pituitary gland of the fetal rat.

The objectives of this study were to determine the first developmental stage when immunoreactive prolactin (PRL) cells appear in the fetal rat anterior pituitary, and then to compare their cytogenesis with that of growth hormone (GH) cells. Immunoreactive PRL cells first appeared on Day 18.5 of gestation, the same stage when GH cells were found to differentiate in the pituitary. During the fetal period, PRL cells were concentrated mainly in the anterior half of the pituitary, whereas GH cells were found predominantly in its posterior half. When estimated as the total sum of immunoreactive areas, the proportion of PRL cells remained low during fetal life in contrast to a marked increase in the GH immunoreactive area. The flip-flop (mirror) section technique revealed that, in the fetal rat, adenohypophysis PRL and GH are contained in different cells. The present study thus indicates that GH and PRL accumulation occurs in independent cells at least in early developmental stages in the rat.

Animals↗
PubMed1992

[Inherited metabolic disorders of the transsulfuration pathway].

Several inherited metabolic disorders of the transsulfuration pathway are discussed. They are hypermethioninemia, homocystinuria, cystathioninuria, beta-mercaptolactate cysteine disulfideuria and sulfite oxidase deficiency (SOD). Primary coverage is given to homocystinuria and SOD. In the case of homocystinuria, metabolic defects include cystathionine beta-synthase deficiency, methylenetetrahydroforate reductase deficiency, and mutations in cobalamin metabolism. Their main clinical pictures, metabolic abnormalities, and treatment are also described. SOD appears in two cases as an isolated enzyme defect and a combined deficiency of sulfite oxidase and xanthine dehydrogenase that share a common molybdenum cofactor. The clinical, biochemical and neurological features of the two disorders are reviewed.

Amino Acid Metabolism, Inborn Errors↗
PubMed1991

Behavioral effects of adenosine agonists: evaluation by punishment, discrete shuttle avoidance and activity tests in mice.

Behavioral effects of propentofylline and N6-(L-2-phenylisopropyl)-adenosine (PIA) were evaluated by operant behavior under a punishment situation, discrete shuttle avoidance response and ambulatory activity in mice. Propentofylline (3 mg/kg, s.c.) and PIA (0.01 mg/kg, s.c.) significantly decreased the punished response without producing a significant change in the non-punished response. Propentofylline and PIA reduced the increase in the punished response induced by caffeine (30 mg/kg) and diazepam (1 mg/kg). Propentofylline and PIA also reduced the increase of ambulation induced by caffeine. Furthermore, the single administration of propentofylline and PIA decreased the response rate and/or % avoidance in the discrete shuttle avoidance situation. However, the effective doses of propentofylline and PIA to reduce the ambulation-increasing effect of caffeine and to produce a change in the avoidance behavior were much higher than those effective for eliciting a significant change in the punished response. The present results suggest that there is an intimate interaction between central adenosine and benzodiazepine systems with regards to the change of punished response.

Adenosine↗
PubMed1989

Anticonflict effect of caffeine: investigation by punishment and hypertonic NaCl solution procedures in mice.

Anticonflict effects of caffeine and diazepam were investigated by two procedures in water-deprived mice; punishment procedure in which the water drinking was suppressed by an electric foot shock, and hypertonic NaCl solution procedure in which the mouse was given 2% NaCl solution in place of tap water to suppress the drinking. Single administration of caffeine (1, 3, 10 and 30 mg/kg) and diazepam (0.25, 0.5, 1 and 2 mg/kg), and combined administration of caffeine (10 mg/kg) plus diazepam (1 mg/kg) were conducted. The mouse's drinking behavior was measured for 40 min immediately after each drug administration. Diazepam 0.25 mg/kg in the punishment procedure, and caffeine 30 mg/kg and diazepam 2 mg/kg in the hypertonic NaCl solution procedure were ineffective to significantly disinhibit the suppressed drinking. However, the other doses of caffeine and diazepam significantly disinhibited the suppressed drinking in both procedures. When the combined administration of caffeine and diazepam was carried out, no enhancement of the effect was observed as compared with those of the single administration of the individual drugs. The present results suggest that caffeine possesses an anticonflict effect at certain doses, although it is pharmacologically different from benzodiazepine anxiolytics.

Animals↗
PubMed1987

Studies on nitrate reductase of Clostridium perfringens. IV. Identification of metals, molybdenum cofactor, and iron-sulfur cluster.

Nitrate reductase of Clostridium perfringens was purified by an improved method using immuno-affinity chromatography. The purified preparation contained Mo, Fe, and acid-labile sulfide; the Mo content was 1 mol per mol and the Fe 3.7 mol per mol of the enzyme. The inactive enzyme obtained from cells grown in the presence of tungstate did not hold Mo but contained 1 mol of W. The content of Fe was not increased. The presence of molybdenum cofactor in the nitrate reductase was indicated by the formation of molybdopterin form A in the oxidation of the enzyme by iodine and by the complementation of NADPH-nitrate reductase with the heart-treated enzyme in the extract of Neurospora crassa nit-1. The Clostridium nitrate reductase had an absorption maximum at 279 nm and shoulders at 320, 380, 430, and 520 nm. This enzyme seems to contain an iron sulfur cluster since the reduced enzyme showed decreased absorption in visible region. The CD spectrum of the enzyme has a positive peak at 425 nm and negative ones at 310, 360, and 595 nm. It was compared with the CD spectrum of ferredoxin (2Fe-2S or 4Fe-4S cluster) and the nitrate reductase of Plectonema boryanum.

Chromatography, Affinity↗
PubMed1986

[Behavioral effects of propentofylline (HWA 285) on ambulatory activity, discrete avoidance response and passive avoidance response in mice].

Behavioral effects of propentofylline (HWA 285) were investigated by means of ambulatory activity, discrete lever-press avoidance and step-through type passive avoidance response in mice. Single administration of HWA 285 produced no marked change in the bodily condition and also produced no changes in ambulatory activity at 1.25-20 mg/kg, s.c.; the discrete avoidance response at 2.5-40 mg/kg, s.c.; and the passive avoidance response at 10-30 mg/kg, s.c. However, 5-20 mg/kg of HWA 285 attenuated the ambulation-increasing effect of methamphetamine (2 mg/kg, s.c.) and scopolamine (0.5 mg/kg, s.c.). HWA 285 tended to attenuate the avoidance-suppressing effect of chlorpromazine (2 mg/kg, s.c.) and physostigmine (0.1 mg/kg, s.c.) at 2.5 mg/kg, while it enhanced the effect of chlorpromazine at 10-40 mg/kg. The mice treated with HWA 285 (10-30 mg/kg) at 30 min before or immediately after the acquisition trial did not show a marked change in the passive avoidance response when the retention trial was done 24 hr after the acquisition trial. The treatment with scopolamine (2 mg/kg, s.c.) at 30 min before the acquisition trial suppressed the passive avoidance response, eliciting a marked shortening of the step-through latency and decrease in % of mice to the 300 sec criterion of latency. The effect of scopolamine was attenuated by combined administration of HWA 285 (30 mg/kg) and treatment with HWA 285 (30 mg/kg) after the end of the acquisition trial. The present results suggest that HWA 285 demonstrates complex behavioral effects which vary dependently on the doses and types of behaviors.

Animals↗