Primary isolation of Mycobacterium tuberculosis on blood agar during the diagnostic process for cat scratch disease.
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Aged↗
Biomedical subjects
H Hahn
Publications and source records attributed to H Hahn.
[Infratentorial pathology a pitfall for neonatal cerebral sonography: Additional sonographic imaging through the posterolateral fontanelle, a prospective study].
BACKGROUND: Routine assessment of the neonatal brain is performed by ultrasound through the anterior fontanelle. The infratentorial structures can be visualised more detailed by sonography through the posterolateral fontanelle. This prospective study was performed to evaluate anterior fontanelle and posterolateral fontanelle for the detection of infratentorial lesions. PATIENTS: Cerebral sonography was performed in 100 preterm infants at the median (range) age of 9 days (0 - 91 days). Median gestational age at birth was 33 weeks (23 - 36 weeks). METHOD: Scanning through the posterolateral fontanelle was performed after sonography through the anterior fontanelle. RESULTS: Neuropathology could be detected in 22 of the 100 preterms. In six patients infratentorial lesions were present in association with supratentorial pathology. The infratentorial abnormalities were clots in the IV. ventricle (n=3), clots in the cisterna magna (n=2), dilation of the IV. ventricle (n=3), dilation of the aqueduct (n=1), and a intraparenchymatous echodensity in the cerebellum (n=1) which receded. When clots were present in the lateral ventricles only the sonography through the posterolateral fontanelle allowed the detection of the infratentorial abnormalities. CONCLUSION: Sonography through the posterolateral fontanelle allows the detection of infratentorial pathology missed by sonography through the anterior fontanelle. Additional scanning through the posterolateral fontanelle should be part of the standard neonatal cerebral sonography.
Cerebellar Diseases↗
Effect of solid lipid nanoparticles (SLN) on cytokine production and the viability of murine peritoneal macrophages.
The purpose of this study was to investigate possible immunomodulatory and cytotoxic effects of solid lipid nanoparticles (SLN) on murine peritoneal macrophages. Immunomodulatory effects of SLN composed of either a lipid- (glycerol-behenate) or a wax (cetylpalmitate) matrix stabilized by the surfactant Poloxamer 188 were analysed by detection of proinflammatory and down-regulatory cytokines in supernatants of thioglycollate-elicited peritoneal macrophages using enzyme-linked immunosorbent assay (ELISA). Cytotoxicity of SLN was assessed using the ITT test. Incubation of macrophages with either SLN at low concentrations did not increase production of interleukin (IL)-6, IL-12, and tumour necrosis factor (TNF)-alpha. At higher SLN concentrations, a concentration-dependent decrease in IL-6 secretion was observed compared to background production of IL-6 by untreated macrophages. IL-12 and TNF-alpha production was neither detected in supernatants of macrophages treated with SLN at any concentration nor in those of untreated cells. The decrease in IL-6 secretion was paralleled by concentration-dependent cytotoxicity of SLN on these cells. In contrast, incubation with polystyrene reference particles neither resulted in decreased IL-6 production nor in a loss of viability. SLN-treated macrophages were found to up-regulate their cytokine production following stimulation with Pansorbin, despite the concentration-dependent cytotoxicity induced by SLN. Down-regulatory effects on SLN-treated macrophages by IL-10 were not observed. In conclusion, incubation of SLN with murine peritoneal macrophages did not induce the production of proinflammatory and down-regulatory cytokines. At high concentrations of SLN, cytotoxic effects on these cells were observed. Cytotoxicity appears to be the main cause of decreased cytokine production by these cells.
Adjuvants, Immunologic↗
The role of cell-mediated immunity in bacterial infections.
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Animals↗
[Increase of bacterial resistance in human medicine by resistance genes of bacteria from meat supplying animals].
Two different groups of bacteria carrying genes encoding for resistance to antibiotics may be transmitted from animals to humans via food products: a.) obligate infectious agents (enteric pathogens, e.g. Salmonella enterica spp., Campylobacter spp., EHEC) and b) facultative pathogenic species (e.g. E. coli, enterococci). Thus far, it is unknown whether genes encoding for resistance to antibiotics from these bacteria may be transferred to bacteria in normal flora of the host. The transfers of genes encoding for resistance to vancomycin from animal sources to the mucosa of humans has been suggested. Thus, there is a threat that these plasmid-encoded resistance genes may also be transferred to other gram-positive organisms present in the human flora. Vancomycin is the antibiotic in reserve for treatment of infections caused by oxacillin (methicillin) resistant strains of S. aureus and by strains of pneumococcus resistant to penicillin.
Animals↗
Circulating VEGF and TGF-beta1 in children with idiopathic nephrotic syndrome.
RATIONALE: The implications of vascular endothelial growth factor (VEGF) and transforming growth factor-beta (TGF-beta) on the development of proteinuria were studied by measuring the mRNA and protein levels of VEGF and TGF-beta1 in 43 children with primary nephrotic syndrome. METHODS: Twenty-seven patients were in the active nephrotic phase at the time of sampling (Group 1), and 16 were in remission (Group 2). In Group 1, 16 were steroid-responders (Group 1a) and 11 were nonresponders (Group 1b). Minimal change lesion (MCL) in 11 patients and focal segmental glomerulosclerosis (FSGS) in 8 were confirmed by renal biopsy. The mRNA expressions of peripheral blood lymphocytes and the plasma levels of proteins were measured by semi-quantitative RT-PCR and ELISA, respectively. RESULTS: Plasma VEGF concentration was higher in Group 1 (204+/-137 pg/mL) than Group 2 (91+/-72 pg/mL) (P=0.002). However, there was no significant difference either between Group 1a (184+/-146 pg/mL) and Group 1b (258+/-134 pg/mL) or between patients with FSGS (330+/-122 pg/mL) and those with MCL (146+/-112 pg/mL). The VEGF mRNA expression showed changes similar to VEGF protein expression, and there was no statistical significance. Plasma levels and mRNA expressions of TGF-beta1 were similar in all groups. CONCLUSIONS: These results suggest that circulating VEGF is associated with proteinuria both in steroid-responsive and steroid-resistant primary nephrotic syndrome in children.
Adolescent↗
[Shoulder girdle and shoulder joint tuberculosis].
From 1955 to 1980 inclusive, 50 patients with tuberculous omarthritis and 11 with specific shoulder girdle disorders (nine sternoclavicular joints, one isolated clavicular shaft focus, as well as one acromioclavicular joint focus) were treated at this clinic with a combined tuberculostatic and surgical therapy. The youngest patient was nine years old, and the oldest patient was 89 years old (36 females, 25 males). The patients (average age 49.4 years) comprised 11 foreign workers. From the beginning of the symptoms up to diagnosis, an average of 1.4 years elapsed. 34 patients (56%) had already been treated earlier for tuberculosis, and 12 (20%) were suffering at the same time from an active specific infection of other localisation. Independently of abscesses and fistulae (35 patients = 57%) as well as other simultaneous tuberculosis, the one-hour value of the erythrocyte sedimentation rate was in the normal range, or it was only marginally to slightly raised (under 20 mm according to W.). In 28 patients (46%), arthrodeses were performed in the shoulder joint, whereas the focus was cleared in the remaining patients. One patient (89 years old) died of senile debility. All complications (16 reactivations, two dislocations of the bone chip implantations, one fail joint) healed without complications after secondary operations. In the follow-up examination (on average, after 3.1 years), we diagnosed one recurrence (sternoclavicular joint process). Of the 45 patients (74%) followed up, 36 (80%) had started working again on average 12 months after the end of hospital treatment, or had resumed their former occupation (pensioners, housewives). Three (7%) of those followed up needed a different occupation or were given a new job within their firm.
Acromioclavicular Joint↗
Reconceptualizing disability: a political science perspective.
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Persons with Disabilities↗
Antibodies against the alpha-factor pheromone of Saccharomyces cerevisiae.
The mating pheromone of baker's yeast, the alpha-factor, is a dodeka-/tridecapeptide, which is not antigenic by itself. It was coupled to succinylated thyroglobulin by the carbodiimide procedure to facilitate selective coupling of the alpha-factor mainly by its N-terminal region. Antibodies against this conjugate were raised in rabbits. After selective precipitation of the rabbit antiserum with succinylated carrier prior to the radial double diffusion test (Ouchterlony) specific antibodies against the coupled alpha-factor could be detected.
Animals↗