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Biomedical subjects

H Hachisuka

Publications and source records attributed to H Hachisuka.

At least 55 records · Page 3Linked to original sources
PubMed1990

Multiple keratoacanthomas with peculiar manifestations and course.

The case of a 60-year-old Japanese man with unique multiple keratoacanthomas is reported. The lesions were located in the perioral area, grew peripherally, and healed centrally. Although individual lesions spontaneously regressed, prompt recurrence was observed after excision. Biopsy specimens taken from early proliferative, fully developed, and regressing stages revealed keratoacanthoma. Involucrin was demonstrated in the lesions during development and resolution. The early stage showed homogeneous staining of moderate intensity in the tumor cells at the suprabasal layers, and the staining became slightly irregular and intense when the lesions were fully developed. During resolution the intensity was markedly diminished. These results suggest that preterminal squamous differentiation accelerated during development of the tumors.

Diagnosis, Differential↗
PubMed1990

Cytofluorometric study of lectin binding to the keratinocytes of epidermolysis bullosa simplex.

Epidermolysis bullosa (EB) simplex is a congenital disease that has blister formation following minor mechanical trauma to the skin. The least amount of information concerning the pathogenesis is known in this disease. One possibility is that there are structural abnormalities in keratinocytes. In the present study, we report the binding of lectin (Ricinus communis agglutinin, Peanut agglutinin, and Soybean agglutinin) to keratinocytes using cytofluorometry. Biopsy skin specimens were taken from patients with simplex, junctional, and dystrophic forms of EB, and normal volunteers. Free keratinocytes were obtained by the treatment of EDTA and trypsin, and fractionated by centrifugation on a continuous colloidal silica (Percoll) density gradient. Fractionated basal cells were stained with biotinyl lectins and avidin-FITC, and measured by cytofluorometry. In all lectins examined, the intensity was low in the basal cells of EB simplex, as compared with normal controls. However, there were no differences among the other forms of EB and normal controls. This results suggest the presence of structural abnormalities in epidermis of EB simplex.

Avidin↗
PubMed1990

Proliferating trichilemmal cyst with spindle cell carcinoma.

We report a 58-year-old woman with proliferating trichilemmal cyst (PTC), from which a spindle cell carcinoma arose. The tumor on her scalp had been removed at another hospital. Histological examination had revealed almost typical features of PTC. However, the case showed a partial transformation to spindle cell carcinoma, and transition zones between squamous epithelium and spindle cells were present. Three months after histologic examination, the patient came to us for the treatment of recurrent tumor. Despite surgical resection, the patient died as a result of distant metastases. Histologically, the recurrent tumor was composed of only spindle-shaped tumor cells. We describe the first example of this uncommon condition.

Carcinoma↗
PubMed1990

Cutaneous phaeohyphomycosis caused by Exophiala jeanselmei after renal transplantation.

A 60-year-old man who had had a renal transplantation developed a nodule on the dorsum of his right middle finger, which was subsequently diagnosed as cutaneous phaeohyphomycosis caused by Exophiala jeanselmei. The patient was successfully treated with 5-fluorocytosine. To our knowledge, this case is the third reported case in the world caused by this species in a postrenal transplant patient that manifested as phaeohyphomycosis.

Dermatomycoses↗
PubMed1990

Expression of ras p21 in mammary and extramammary Paget's disease.

With the use of immunohistochemical techniques, we examined the expression of ras oncogene product p21 in 4 cases of mammary and 13 cases of extramammary Paget's disease. In every mammary case, positive immunostaining was observed in Paget's cells and the underlying tumor (in the 3 cases where a tumor was present). Among the extramammary cases, the cells in 6 cases were immunoreactive. In 4 of these positive extramammary cases, dermal invasion and metastases of regional lymph nodes were observed. Another 2 extramammary cases were weakly reactive. An enhanced expression of ras p21 therefore seems to depend on the region of the tumor or on the biologic behavior. This work suggested that an enhanced expression of ras p21 in Paget's cells may represent a new clinical marker for tumors in cases of mammary and extramammary Paget's disease.

Aged↗
PubMed1990

[Relationship between molecular structure of halogenated methane compounds and their hypotensive effect in rats].

In this study gases of twelve halogenated methane compounds were administered by inspiration to Wistar rats in order to elucidate the relationship between molecular structure of the compounds and their hypotensive effect, and to develop new inhalational circulatory control agents. The correlation between the hypotensive effect of the compounds evaluated from the experimental data and computed van der Waals volume as well as surface area following molecular mechanics calculations of structure and energy, was investigated. The results showed that the compounds with van der Waals volume and surface area more than 73 A3 and 100 A2, respectively, had the hypotensive effect. In conclusion, our data suggest that the hypotensive effect of inhalational agents requires greater molecular sizes than regular ones.

Administration, Inhalation↗
PubMed1989

Cytofluorometric study on lectin binding of isolated guinea pig keratinocytes.

Lectin binding was cytofluorometrically measured on fractionated keratinocytes of guinea pig. Free keratinocytes were obtained by treatment of EDTA and trypsin. After the treatment, they were separated into 3 fractions by centrifugation on a continuous colloidal silica (Percoll) density gradient. Cells in each fraction were stained by biotinyl lectins and avidin-FITC, and fluorescence intensity was measured by cytofluorometry. Results obtained indicate that little cell surface glycoconjugate is lost during the preparation of free keratinocytes.

Animals↗
PubMed1989

Immunohistochemical demonstration of epithelial membrane antigen (EMA), carcinoembryonic antigen (CEA), and keratin on mammary and extramammary Paget's disease.

We examined the presence and the distribution of epithelial membrane antigen (EMA), carcinoembryonic antigen (CEA), and keratin in 4 cases (6 specimens) of mammary Paget's disease and 9 cases (18 specimens) of extramammary Paget's disease utilizing immunoperoxidase technique. Paget's cells in 23 out of 24 specimens were demonstrable for EMA. Positive staining was observed for CEA in every specimen. Among the 24 specimens, 16 showed positivity for keratin. In addition, CEA was positive in Paget's cells and the staining for CEA was stronger than that of EMA and keratin. On the other hand, some Paget's cells were negative for EMA of keratin although other positive cells were observed in the same sections. This findings indicates that Paget's cells might express CEA and/or EMA and/or keratin depending on their differentiation. EMA is present in most organs showing glandular differentiation, and anti-keratin antibody used in this study recognizes not only keratinocytes but glandular cells. Thus, our study suggests Paget's cells are of glandular origin.

Antigens↗
PubMed1989

The existence of a platelet-activating factor (Paf-acether)-like substance in blister fluid derived from patients with bullous pemphigoid as demonstrated by human platelet aggregation techniques.

We investigated the presence of a platelet-activating factor-like substance in blister fluid obtained from patients with bullous pemphigoid. Human platelet aggregation activity was present in blister fluids obtained from 4 out of 6 patients with bullous pemphigoid and in blister fluids obtained from 3 cases with contact dermatitis. Platelet aggregation activity of pemphigoid blister fluids was inhibited by pretreatment with lyso-PAF, which is a precursor metabolite of platelet activating factor. The activity of blister fluids obtained from patients suffering from contact dermatitis was not inhibited by precursor. Blister fluid obtained from burn lesions and blisters of normal skin induced in the suction revealed no platelet aggregation activity. These results suggest that platelet-activating factor or a similar substance is present in the blister fluid obtained from suffered bullous pemphigoid.

Adult↗
PubMed1988

Purification of rat cutaneous mast cells with Percoll density centrifugation.

The skin is the major site on anaphylaxis, and cutaneous mast cells have an important role in its reactions. The isolation and purification of rat cutaneous mast cells are described here. Rat abdominal skin was digested with collagenase and hyaluronidase, and centrifuged with Percoll. The buoyant density of cutaneous mast cells was high, and relatively pure mast cells were obtained. The purity of cutaneous mast cells was 74% +/- 2.4% before and 50.0% +/- 6.4% after Percoll density centrifugation; peritoneal mast cells revealed 5.8% +/- 1.3% purity before and 61.0% +/-10.6% purity after the same procedure. The isolated cutaneous cells released 21.3% +/- 3.8% histamine and the peritoneal mast cells released 55.5% +/- 3.8% histamine upon stimulation with 10 micrograms/ml compound 48/80. These findings suggest that there are functional subsets of connective tissue mast cells.

Animals↗
PubMed1988

Statistical survey of 150 cases with sporotrichosis.

A survey of 150 cases with sporotrichosis seen at the Dermatological Clinic of Kurume University Hospital from February 1962 to October 1986 was reported. The proportion of cases with sporotrichosis to the total number of outpatients was 0.17%. Greater percentage of cases fell into the less than 10 years old or more than 40 years old groups. The male to female ratio was 1:1.46, and 38 cases occurred in farmers. Geographic distribution was remarkable, especially around the Chikugo and Yabe river. Sixty-four cases showed the cutaneous lymphangitic type and 85 cases the localized cutaneous type and one case atypical type. The face and upper extremities were the most affected. The sporotrichin test was positive in 117 of 131 cases. The causative organism was demonstrated in tissue sections in 69% of the cases.

Adolescent↗
PubMed1988

Effect of antianaphylactic agents on substance-P induced histamine release from rat peritoneal mast cells.

Substance P is known to be a potent histamine liberator for mast cells. The influence of antianaphylactic agents, disodium cromoglycate (DSCG), ketotifen, and tranilast was studied on substance-P and compound 48/80-induced histamine release from rat peritoneal mast cells. Substance-P induced histamine release was inhibited by these agents, while compound 48/80-induced histamine release was not inhibited by tranilast. Our findings suggest that these antianaphylactic agents are assumed to be effective for cutaneous diseases which might be concerned with substance P and histamine.

Animals↗
PubMed1988

Abnormal binding of lectin to the epidermal cell membranes in the skin of epidermolysis bullosa simplex.

Epidermolysis bullosa (EB) simplex is a congenital disease that has blister formation following minor mechanical trauma to the skin. However, the least amount of information concerning the pathogenesis is known in this condition. One possibility is that there are structural abnormalities in epidermal cell membranes. In the present study, we examined the binding of lectins to epidermal cell membranes by ABC method. Biopsy skin specimens were obtained from patients with simplex, junctional and dystrophic forms of EB, and normal volunteers. In the case of EB simplex, the cell membranes of keratinocytes in the basal and spinous cell layers did not bind to soybean agglutinin in specimens from blister edges or mechanical traumatized areas. No differences were found in binding pattern between the other forms of EB and normal controls. This results suggest the presence of structural abnormalities in epidermis of EB simplex.

Cell Membrane↗
PubMed1988

Immunohistochemical observation of S-100 protein and neuron specific enolase in the tumour cells of granular cell tumour.

An immunohistochemical technique for the detection of S-100 protein, neuron specific enolase (NSE), carcinoembryonic antigen (CEA) and muramidase (lysozyme) was applied to a case of the granular cell tumour. S-100 protein was detected both in the nuclei and cytoplasma of the granular cells, and NSE was weakly positive in their cytoplasms. CEA and lysozyme were negative in the tumour cells. Our results supports the concept that granular cell tumours are derived from Schwann cells.

Adult↗